Restriction map for
pF1KB7154
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaHI BsgI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI Ksp632I
KspI MfeI MluI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 337 GTCTAC GT^[AC][GT]AC
AccIII 158 TCCGGA T^CCGGA
AciI 366 CCGC CCGC(-3/-1)
AfaI 503 GTAC GT^AC
AocI 1033 CCTGAGG CC^T[ACGT]AGG
Asp700I 573 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 43 GTGCTC G[AT]GC[AT]^C
BclI 344 TGATCA T^GATCA
BsaAI 719 TACGTG [CT]AC^GT[GA]
BsaI 246 GGTCTC GGTCTC(1/5)
BsaWI 158 TCCGGA [AT]^CCGG[AT]
BseAI 158 TCCGGA T^CCGGA
BsiHKAI 43 GTGCTC G[AT]GC[AT]^C
BsmBI 1039 GAGACG CGTCTC(1/5)
BspEI 158 TCCGGA T^CCGGA
BstYI 77 GGATCT [GA]^GATC[CT]
Bsu36I 1033 CCTGAGG CC^T[ACGT]AGG
Eco81I 1033 CCTGAGG CC^T[ACGT]AGG
EcoNI 972 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 344 TGATCA T^GATCA
HapII 159 CCGG C^CGG
HincII 175 GTCAAC GT[CT]^[GA]AC
HindII 175 GTCAAC GT[CT]^[GA]AC
HpaII 159 CCGG C^CGG
MflI 77 GGATCT [GA]^GATC[CT]
MroI 158 TCCGGA T^CCGGA
MspI 159 CCGG C^CGG
NcoI 587 CCATGG C^CATGG
PshAI 975 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 882 CAGCTG CAG^CTG
RsaI 503 GTAC GT^AC
ScaI 502 AGTACT AGT^ACT
SfaNI 384 GCATC GCATC(5/9)
SfcI 560 CTGTAG C^T[GA][CT]AG
SpeI 955 ACTAGT A^CTAGT
XhoII 77 GGATCT [GA]^GATC[CT]
XmnI 573 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 477 AAATTT [GA]^AATT[CT]
680 AAATTC
AflIII 575 ACGTGT A^C[GA][CT]GT
720 ACGTGT
ApaI 395 GGGCCC GGGCC^C
968 GGGCCC
ApoI 477 AAATTT [GA]^AATT[CT]
680 AAATTC
BanII 395 GGGCCC G[GA]GC[CT]^C
968 GGGCCC
BbsI 112 GTCTTC GAAGAC(2/6)
724 GTCTTC
BpuAI 112 GTCTTC GAAGAC(2/6)
724 GTCTTC
BsaBI 312 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
345 GATCACCATC
BseRI 37 GAGGAG GAGGAG(10/8)
1036 GAGGAG
BsmAI 1039 GAGAC GTCTC(1/5)
247 GTCTC
BsmI 541 GCATTC GAATGC(1/-1)
782 GCATTC
BstXI 413 CCATGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
953 CCACTAGTTTGG
DsaI 96 CCACGG C^C[GA][CT]GG
587 CCATGG
MamI 312 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
345 GATCACCATC
MspA1I 366 CCGCTG C[AC]G^C[GT]G
882 CAGCTG
MwoI 45 GCTCCCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
733 GCTGTGGTGGC
PpuMI 119 GGGTCCT [GA]G^G[AT]CC[CT]
980 AGGTCCC
TfiI 155 GATTC G^A[AT]TC
939 GATTC
Tsp45I 103 GTCAC ^GT[GC]AC
551 GTGAC
XcmI 350 CCATCATTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
612 CCACTTCATTATTGG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AluI AlwI AvaII BalI BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrDI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MvaI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI StyI
Tru9I TseI Tsp509I TspRI