Restriction map for
pF1KB7150
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccIII AciI AclI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbsI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BssHII BssSI BstBI
BstEII BstPI Bsu36I CelII CfoI
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tsp45I Tth111I
Van91I XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 918 GTATAC GT^[AC][GT]AC
AccII 979 CGCG CG^CG
AcsI 904 GAATTC [GA]^AATT[CT]
Alw44I 998 GTGCAC G^TGCAC
AlwI 799 GATCC GGATC(4/5)
ApaI 825 GGGCCC GGGCC^C
ApaLI 998 GTGCAC G^TGCAC
ApoI 904 GAATTC [GA]^AATT[CT]
BalI 875 TGGCCA TGG^CCA
BbvCI 655 GCTGAGG CCTCAGC(-5/-2)
BcgI 644 GCAACTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
Bsp1407I 368 TGTACA T^GTACA
BsrFI 994 GCCGGT [GA]^CCGG[CT]
BsrGI 368 TGTACA T^GTACA
Bst1107I 918 GTATAC GTA^TAC
BstAPI 357 GCATTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 979 CGCG CG^CG
BstXI 746 CCAAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 918 GTATAC GTA^TAC
Cfr10I 994 GCCGGT [GA]^CCGG[CT]
DsaI 183 CCATGG C^C[GA][CT]GG
EaeI 875 TGGCCA [CT]^GGCC[GA]
EcoRI 904 GAATTC G^AATTC
HapII 995 CCGG C^CGG
HpaII 995 CCGG C^CGG
HphI 756 GGTGA GGTGA(8/7)
MaeII 1002 ACGT A^CGT
MaeIII 421 GTAAC ^GT[ACGT]AC
MluNI 875 TGGCCA TGG^CCA
MscI 875 TGGCCA TGG^CCA
MslI 526 CACATCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 224 CAGCTG C[AC]G^C[GT]G
MspI 995 CCGG C^CGG
MvnI 979 CGCG CG^CG
NcoI 183 CCATGG C^CATGG
PaeR7I 5 CTCGAG C^TCGAG
PleI 254 GAGTC GAGTC(4/5)
PstI 1036 CTGCAG CTGCA^G
PvuII 224 CAGCTG CAG^CTG
SspBI 368 TGTACA T^GTACA
TaqI 6 TCGA T^CGA
TfiI 109 GAATC G^A[AT]TC
TthHB8I 6 TCGA T^CGA
XbaI 297 TCTAGA T^CTAGA
XhoI 5 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaI 5 CTCGAG C^[CT]CG[GA]G
53 CCCGAG
BbvI 967 GCAGC GCAGC(8/12)
226 GCTGC
BglII 294 AGATCT A^GATCT
552 AGATCT
BpmI 478 CTGGAG CTGGAG(16/14)
772 CTGGAG
BsoBI 5 CTCGAG C^[CT]CG[GA]G
53 CCCGAG
BstYI 294 AGATCT [GA]^GATC[CT]
552 AGATCT
DraII 324 AGGCCCT [GA]G^G[ACGT]CC[CT]
824 GGGGCCC
EcoO109I 324 AGGCCCT [GA]G^G[ACGT]CC[CT]
824 GGGGCCC
Fnu4HI 226 GCTGC GC^[ACGT]GC
967 GCAGC
HincII 256 GTCAAC GT[CT]^[GA]AC
541 GTCAAC
HindII 256 GTCAAC GT[CT]^[GA]AC
541 GTCAAC
HinfI 109 GAATC G^A[ACGT]TC
254 GAGTC
ItaI 226 GCTGC GC^[ACGT]GC
967 GCAGC
MflI 294 AGATCT [GA]^GATC[CT]
552 AGATCT
MseI 680 TTAA T^TAA
1013 TTAA
SmlI 5 CTCGAG C^T[CT][GA]AG
865 CTTGAG
Tru9I 680 TTAA T^TAA
1013 TTAA
TseI 226 GCTGC G^C[AT]GC
967 GCAGC
XhoII 294 AGATCT [GA]^GATC[CT]
552 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI AspHI BanII BfaI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I FokI HaeIII
Ksp632I MaeI MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI Tsp509I TspRI