Restriction map for
pF1KB7146
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BalI BamHI
BanI BbeI BbvCI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AhdI 51 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 358 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 51 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 32 CACGTG CAC^GTG
BbsI 339 GAAGAC GAAGAC(2/6)
BciVI 112 GTATCC GTATCC(6/5)
BfaI 1019 CTAG C^TAG
BglII 665 AGATCT A^GATCT
BpuAI 339 GAAGAC GAAGAC(2/6)
BsaI 604 GGTCTC GGTCTC(1/5)
BseRI 658 GAGGAG GAGGAG(10/8)
Bsp1407I 374 TGTACA T^GTACA
BspHI 635 TCATGA T^CATGA
BsrGI 374 TGTACA T^GTACA
BssSI 578 CTCGTG CACGAG(-5/-1)
BstF5I 122 CATCC GGATG(2/0)
DraI 501 TTTAAA TTT^AAA
DraII 860 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 173 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 526 GACTCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 989 CCATGG C^C[GA][CT]GG
Eam1105I 51 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 860 AGGTCCT [GA]G^G[ACGT]CC[CT]
FokI 122 CATCC GGATG(9/13)
HapII 54 CCGG C^CGG
HpaII 54 CCGG C^CGG
MaeI 1019 CTAG C^TAG
MspI 54 CCGG C^CGG
MwoI 729 GCCCAGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 989 CCATGG C^CATGG
NheI 1018 GCTAGC G^CTAGC
PflMI 747 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 32 CACGTG CAC^GTG
PmlI 32 CACGTG CAC^GTG
PpuMI 860 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 959 CTGCAG CTGCA^G
RcaI 635 TCATGA T^CATGA
SapI 1025 GCTCTTC GCTCTTC(1/4)
SmaI 53 CCCGGG CCC^GGG
SmlI 184 CTCAAG C^T[CT][GA]AG
SspBI 374 TGTACA T^GTACA
Van91I 747 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 53 CCCGGG C^CCGGG
XmaI 53 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 673 GAATTC [GA]^AATT[CT]
814 GAATTC
ApoI 673 GAATTC [GA]^AATT[CT]
814 GAATTC
AvaI 5 CTCGGG C^[CT]CG[GA]G
53 CCCGGG
AvaII 826 GGTCC G^G[AT]CC
861 GGTCC
BanII 727 GAGCCC G[GA]GC[CT]^C
833 GAGCCC
BcnI 53 CCCGG CC^[GC]GG
54 CCGGG
BpmI 772 CTGGAG CTGGAG(16/14)
865 CTGGAG
BsaAI 32 CACGTG [CT]AC^GT[GA]
173 CACGTA
BsmAI 315 GTCTC GTCTC(1/5)
605 GTCTC
BsmFI 568 GGGAC GGGAC(10/14)
895 GTCCC
BsoBI 5 CTCGGG C^[CT]CG[GA]G
53 CCCGGG
BsrI 899 CCAGT ACTGG(1/-1)
909 CCAGT
EcoRI 673 GAATTC G^AATTC
814 GAATTC
HaeIII 876 GGCC GG^CC
1011 GGCC
MaeII 33 ACGT A^CGT
174 ACGT
MaeIII 171 GTCAC ^GT[ACGT]AC
574 GTGAC
NciI 53 CCCGG CC^[GC]GG
54 CCGGG
SfcI 221 CTGTAG C^T[GA][CT]AG
959 CTGCAG
TfiI 514 GATTC G^A[AT]TC
615 GAATC
Tsp45I 171 GTCAC ^GT[GC]AC
574 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AfaI AluI AlwI Asp700I AspHI
BbvI BmyI BsaJI BsgI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI HindIII
HinfI HphI ItaI Ksp632I MboI
MboII MflI MnlI MseI MslI
MvaI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI StyI
Tru9I TseI Tsp509I TspRI XhoII
XmnI