Restriction map for
pF1KB7142
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoT22I FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AfaI 591 GTAC GT^AC
AflII 703 CTTAAG C^TTAAG
AvaII 530 GGACC G^G[AT]CC
BfrI 703 CTTAAG C^TTAAG
BglII 234 AGATCT A^GATCT
BsaAI 751 TACGTA [CT]AC^GT[GA]
BsaI 186 GAGACC GGTCTC(1/5)
BsiYI 624 CCTCCCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 624 CCTCCCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI 149 GCAATG GCAATG(2/0)
BssSI 133 CTCGTG CACGAG(-5/-1)
BstEII 889 GGTAACC G^GT[ACGT]ACC
BstPI 889 GGTAACC G^GT[ACGT]ACC
Cfr13I 530 GGACC G^G[ACGT]CC
DsaI 678 CCATGG C^C[GA][CT]GG
EcoO65I 889 GGTAACC G^GT[ACGT]ACC
EcoRV 162 GATATC GAT^ATC
FauI 965 GCGGG CCCGC(4/6)
HaeIII 225 GGCC GG^CC
HindIII 845 AAGCTT A^AGCTT
MaeII 752 ACGT A^CGT
NcoI 678 CCATGG C^CATGG
NlaIV 968 GGAACC GG[ACGT]^[ACGT]CC
RsaI 591 GTAC GT^AC
SapI 195 GAAGAGC GCTCTTC(1/4)
Sau96I 530 GGACC G^G[ACGT]CC
SnaBI 751 TACGTA TAC^GTA
Tsp45I 640 GTGAC ^GT[GC]AC
XbaI 859 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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BclI 401 TGATCA T^GATCA
512 TGATCA
BfaI 13 CTAG C^TAG
860 CTAG
BsgI 228 CTGCAC GTGCAG(16/14)
446 GTGCAG
BsmAI 186 GAGAC GTCTC(1/5)
548 GTCTC
BssKI 145 CCAGG ^CC[ACGT]GG
222 CCTGG
BstNI 145 CCAGG CC^[AT]GG
222 CCTGG
EarI 364 CTCTTC CTCTTC(1/4)
195 GAAGAG
EcoRII 145 CCAGG ^CC[AT]GG
222 CCTGG
FbaI 401 TGATCA T^GATCA
512 TGATCA
Ksp632I 364 CTCTTC CTCTTC(1/4)
195 GAAGAG
MaeI 13 CTAG C^TAG
860 CTAG
MseI 584 TTAA T^TAA
704 TTAA
MspA1I 53 CAGCTG C[AC]G^C[GT]G
696 CAGCTG
MvaI 145 CCAGG CC^[AT]GG
222 CCTGG
PvuII 53 CAGCTG CAG^CTG
696 CAGCTG
ScrFI 145 CCAGG CC^[ACGT]GG
222 CCTGG
Tru9I 584 TTAA T^TAA
704 TTAA
Tsp509I 67 AATT ^AATT
990 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsrI BstF5I BstYI Cac8I DpnII
EcoT14I Fnu4HI FokI HinfI HphI
ItaI MaeIII MboI MboII MflI
MnlI MslI MwoI NdeII NlaIII
Sau3AI SfaNI SmlI StyI TaqI
TfiI TseI TspRI TthHB8I XhoII