Restriction map for
pF1KB7132
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I CelII
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco52I EcoRI
EcoT22I FauI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MaeI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 652 CGCG CG^CG
AcyI 789 GGCGCC G[GA]^CG[CT]C
AlwI 802 GATCC GGATC(4/5)
AocI 193 CCTGAGG CC^T[ACGT]AGG
Aor51HI 23 AGCGCT AGC^GCT
ApaI 836 GGGCCC GGGCC^C
Asp700I 40 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 712 GAGCAC G[AT]GC[AT]^C
BanII 836 GGGCCC G[GA]GC[CT]^C
BbeI 789 GGCGCC GGCGC^C
BsaHI 789 GGCGCC G[GA]^CG[CT]C
BsiHKAI 712 GAGCAC G[AT]GC[AT]^C
BsmFI 315 GTCCC GGGAC(10/14)
BsrDI 266 GCAATG GCAATG(2/0)
BstEII 170 GGTCACC G^GT[ACGT]ACC
BstPI 170 GGTCACC G^GT[ACGT]ACC
BstUI 652 CGCG CG^CG
Bsu36I 193 CCTGAGG CC^T[ACGT]AGG
DraIII 231 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 525 CGGCCA [CT]^GGCC[GA]
Eco47III 23 AGCGCT AGC^GCT
Eco81I 193 CCTGAGG CC^T[ACGT]AGG
EcoNI 147 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 170 GGTCACC G^GT[ACGT]ACC
EcoRV 454 GATATC GAT^ATC
Hin1I 789 GGCGCC G[GA]^CG[CT]C
KasI 789 GGCGCC G^GCGCC
MspA1I 95 CAGCTG C[AC]G^C[GT]G
MvnI 652 CGCG CG^CG
NaeI 523 GCCGGC GCC^GGC
NarI 789 GGCGCC GG^CGCC
NgoMIV 523 GCCGGC G^CCGGC
PvuII 95 CAGCTG CAG^CTG
SfaNI 115 GCATC GCATC(5/9)
SfoI 789 GGCGCC GGC^GCC
SphI 479 GCATGC GCATG^C
StuI 42 AGGCCT AGG^CCT
XmnI 40 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 593 GTAC GT^AC
780 GTAC
BbvCI 34 GCTGAGG CCTCAGC(-5/-2)
97 GCTGAGG
BclI 134 TGATCA T^GATCA
585 TGATCA
BmyI 712 GAGCAC G[AGT]GC[ACT]^C
836 GGGCCC
BsgI 82 GTGCAG GTGCAG(16/14)
760 GTGCAG
Bsp1286I 712 GAGCAC G[AGT]GC[ACT]^C
836 GGGCCC
BspMI 547 ACCTGC ACCTGC(4/8)
483 GCAGGT
DraII 835 AGGGCCC [GA]G^G[ACGT]CC[CT]
836 GGGCCCT
EcoO109I 835 AGGGCCC [GA]G^G[ACGT]CC[CT]
836 GGGCCCT
EcoT14I 54 CCAAGG C^C[AT][AT]GG
832 CCAAGG
FbaI 134 TGATCA T^GATCA
585 TGATCA
HaeII 23 AGCGCT [GA]GCGC^[CT]
789 GGCGCC
MseI 386 TTAA T^TAA
637 TTAA
MslI 480 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
498 CATTGATGTG
MwoI 3 GCTTCTTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
732 GCTCAGGAAGC
NspI 479 GCATGC [GA]CATG^[CT]
873 GCATGT
RsaI 593 GTAC GT^AC
780 GTAC
SmlI 148 CTTGAG C^T[CT][GA]AG
415 CTTGAG
StyI 54 CCAAGG C^C[AT][AT]GG
832 CCAAGG
TfiI 87 GATTC G^A[AT]TC
163 GATTC
Tru9I 386 TTAA T^TAA
637 TTAA
Tsp509I 384 AATT ^AATT
433 AATT
TspRI 717 CAGTG CA[GC]TG[ACGT][ACGT]^
798 CAGTG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BcnI BpmI BsaJI BseRI BsiYI
BslI BsrFI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI TseI Tsp45I