Restriction map for
pF1KB7115
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BanII
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 512 GGCGCC G[GA]^CG[CT]C
AfaI 219 GTAC GT^AC
Aor51HI 566 AGCGCT AGC^GCT
BalI 14 TGGCCA TGG^CCA
BamHI 446 GGATCC G^GATCC
BbeI 512 GGCGCC GGCGC^C
BclI 302 TGATCA T^GATCA
BcnI 704 CCCGG CC^[GC]GG
BglI 694 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 512 GGCGCC G[GA]^CG[CT]C
BsgI 480 CTGCAC GTGCAG(16/14)
BsiEI 335 CGGCCG CG[GA][CT]^CG
BsmAI 274 GAGAC GTCTC(1/5)
BsmBI 274 GAGACG CGTCTC(1/5)
BssSI 507 CACGAG CACGAG(-5/-1)
BstXI 785 CCAGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 446 GGATCC [GA]^GATC[CT]
EagI 335 CGGCCG C^GGCCG
EclXI 335 CGGCCG C^GGCCG
Eco47III 566 AGCGCT AGC^GCT
Eco52I 335 CGGCCG C^GGCCG
EcoNI 243 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 302 TGATCA T^GATCA
Hin1I 512 GGCGCC G[GA]^CG[CT]C
HinfI 742 GACTC G^A[ACGT]TC
KasI 512 GGCGCC G^GCGCC
MaeIII 253 GTCAC ^GT[ACGT]AC
MflI 446 GGATCC [GA]^GATC[CT]
MluNI 14 TGGCCA TGG^CCA
MscI 14 TGGCCA TGG^CCA
MseI 173 TTAA T^TAA
NarI 512 GGCGCC GG^CGCC
NciI 704 CCCGG CC^[GC]GG
NlaIII 474 CATG CATG^
NspI 473 GCATGC [GA]CATG^[CT]
PleI 742 GACTC GAGTC(4/5)
PpuMI 676 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 219 GTAC GT^AC
SfoI 512 GGCGCC GGC^GCC
SphI 473 GCATGC GCATG^C
TaqI 377 TCGA T^CGA
Tru9I 173 TTAA T^TAA
Tsp45I 253 GTCAC ^GT[GC]AC
TthHB8I 377 TCGA T^CGA
XhoII 446 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AluI 58 AGCT AG^CT
263 AGCT
AlwI 447 GATCC GGATC(4/5)
446 GGATC
AlwNI 79 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
612 CAGCCTCTG
AvaI 72 CTCGGG C^[CT]CG[GA]G
807 CTCGGG
AvaII 6 GGACC G^G[AT]CC
677 GGTCC
BanI 438 GGCACC G^G[CT][GA]CC
512 GGCGCC
BpmI 244 CTGGAG CTGGAG(16/14)
259 CTGGAG
BsoBI 72 CTCGGG C^[CT]CG[GA]G
807 CTCGGG
BstAPI 473 GCATGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
611 GCAGCCTCTGC
DdeI 400 CTGAG C^T[ACGT]AG
551 CTGAG
DraII 67 GGGGCCT [GA]G^G[ACGT]CC[CT]
676 GGGTCCC
DsaI 407 CCACGG C^C[GA][CT]GG
461 CCACGG
EcoO109I 67 GGGGCCT [GA]G^G[ACGT]CC[CT]
676 GGGTCCC
HapII 271 CCGG C^CGG
705 CCGG
HpaII 271 CCGG C^CGG
705 CCGG
MboII 161 TCTTC GAAGA(8/7)
164 TCTTC
MslI 180 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
468 CATCTGCATG
MspI 271 CCGG C^CGG
705 CCGG
PstI 280 CTGCAG CTGCA^G
609 CTGCAG
SfcI 280 CTGCAG C^T[GA][CT]AG
609 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AspHI BbvI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HgaI HhaI
HinP1I ItaI MboI MnlI MspA1I
MvaI MvnI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI