Restriction map for
pF1KB7049
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaHI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StyI SwaI Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 624 GCGG CCGC(-3/-1)
AcsI 1249 AAATTT [GA]^AATT[CT]
ApoI 1249 AAATTT [GA]^AATT[CT]
Asp700I 328 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1009 GAGCTC G[AT]GC[AT]^C
AvaI 1277 CCCGGG C^[CT]CG[GA]G
BanI 15 GGTGCC G^G[CT][GA]CC
BbrPI 458 CACGTG CAC^GTG
BglII 1390 AGATCT A^GATCT
BsaAI 458 CACGTG [CT]AC^GT[GA]
BsaBI 595 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1462 GGTCTC GGTCTC(1/5)
BsaWI 1459 TCCGGT [AT]^CCGG[AT]
BseRI 1465 CTCCTC GAGGAG(10/8)
BsgI 473 GTGCAG GTGCAG(16/14)
BsiHKAI 1009 GAGCTC G[AT]GC[AT]^C
BsoBI 1277 CCCGGG C^[CT]CG[GA]G
BsrDI 1228 GCAATG GCAATG(2/0)
BstF5I 1124 GGATG GGATG(2/0)
DraII 66 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 953 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 75 GACTATATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 66 AGGCCCC [GA]G^G[ACGT]CC[CT]
FokI 1124 GGATG GGATG(9/13)
HindIII 1427 AAGCTT A^AGCTT
MamI 595 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1170 CATGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI 458 CACGTG CAC^GTG
PmlI 458 CACGTG CAC^GTG
SacI 1009 GAGCTC GAGCT^C
SapI 1309 GAAGAGC GCTCTTC(1/4)
ScaI 401 AGTACT AGT^ACT
SexAI 995 ACCAGGT A^CC[AT]GGT
SfcI 180 CTACAG C^T[GA][CT]AG
SmaI 1277 CCCGGG CCC^GGG
SmlI 637 CTCAAG C^T[CT][GA]AG
SphI 632 GCATGC GCATG^C
XmaCI 1277 CCCGGG C^CCGGG
XmaI 1277 CCCGGG C^CCGGG
XmnI 328 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AhdI 805 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1455 GACTTCCGGTC
AspEI 805 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1455 GACTTCCGGTC
AvaII 83 GGTCC G^G[AT]CC
216 GGTCC
BalI 1024 TGGCCA TGG^CCA
1062 TGGCCA
BamHI 226 GGATCC G^GATCC
1413 GGATCC
BanII 1009 GAGCTC G[GA]GC[CT]^C
1074 GGGCTC
BcnI 1277 CCCGG CC^[GC]GG
1278 CCGGG
BfaI 1315 CTAG C^TAG
1336 CTAG
Bsp1407I 498 TGTACA T^GTACA
561 TGTACA
BspHI 591 TCATGA T^CATGA
1005 TCATGA
BsrGI 498 TGTACA T^GTACA
561 TGTACA
Cac8I 632 GCATGC GC[ACGT]^[ACGT]GC
1090 GCAAGC
Eam1105I 805 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1455 GACTTCCGGTC
HapII 1278 CCGG C^CGG
1460 CCGG
HpaII 1278 CCGG C^CGG
1460 CCGG
MaeI 1315 CTAG C^TAG
1336 CTAG
MluNI 1024 TGGCCA TGG^CCA
1062 TGGCCA
MscI 1024 TGGCCA TGG^CCA
1062 TGGCCA
MspI 1278 CCGG C^CGG
1460 CCGG
MwoI 45 GCTGAAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
636 GCTCAAGGAGC
NciI 1277 CCCGG CC^[GC]GG
1278 CCGGG
PflMI 140 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
906 CCATGAGGTGG
RcaI 591 TCATGA T^CATGA
1005 TCATGA
SfaNI 249 GATGC GCATC(5/9)
1125 GATGC
SspBI 498 TGTACA T^GTACA
561 TGTACA
StuI 741 AGGCCT AGG^CCT
1158 AGGCCT
TaqI 546 TCGA T^CGA
1196 TCGA
TfiI 1166 GATTC G^A[AT]TC
1185 GAATC
Tsp45I 667 GTCAC ^GT[GC]AC
1145 GTCAC
TthHB8I 546 TCGA T^CGA
1196 TCGA
Van91I 140 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
906 CCATGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AfaI AluI AlwI BbvI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI BstYI
Cfr13I DdeI DpnII EaeI EarI
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI Tru9I
TseI Tsp509I TspRI XhoII