Restriction map for
pF1KB4809
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AvrII BamHI
BbsI BciVI BclI BfrI BglII
BlnI BpuAI BsaI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BstBI Bsu36I Cfr10I ClaI
DraI Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KspI MfeI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI SacI SacII SalI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tru9I Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1204 GTATAC GT^[AC][GT]AC
AcsI 1275 GAATTT [GA]^AATT[CT]
AflIII 1208 ACACGT A^C[GA][CT]GT
ApaI 1775 GGGCCC GGGCC^C
ApoI 1275 GAATTT [GA]^AATT[CT]
AspI 763 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 562 CTCGAG C^[CT]CG[GA]G
AviII 1850 TGCGCA TGC^GCA
BbeI 608 GGCGCC GGCGC^C
BbrPI 1618 CACGTG CAC^GTG
BbvCI 1866 CCTCAGC CCTCAGC(-5/-2)
BfaI 1138 CTAG C^TAG
BlpI 2013 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2013 GCTGAGC GC^T[ACGT]AGC
BsaAI 1618 CACGTG [CT]AC^GT[GA]
BsaBI 1548 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1960 TCCGGT [AT]^CCGG[AT]
BsgI 1615 CTGCAC GTGCAG(16/14)
BsmBI 1489 GAGACG CGTCTC(1/5)
BsmI 407 GCATTC GAATGC(1/-1)
BsoBI 562 CTCGAG C^[CT]CG[GA]G
Bsp1407I 263 TGTACA T^GTACA
BsrGI 263 TGTACA T^GTACA
BssSI 1786 CACGAG CACGAG(-5/-1)
Bst1107I 1204 GTATAC GTA^TAC
BstXI 1298 CCATGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1204 GTATAC GTA^TAC
CelII 2013 GCTGAGC GC^T[ACGT]AGC
CpoI 479 CGGTCCG CG^G[AT]CCG
EagI 1476 CGGCCG C^GGCCG
EarI 1927 CTCTTC CTCTTC(1/4)
EclXI 1476 CGGCCG C^GGCCG
Eco52I 1476 CGGCCG C^GGCCG
FspI 1850 TGCGCA TGC^GCA
HinfI 8 GATTC G^A[ACGT]TC
KasI 608 GGCGCC G^GCGCC
Ksp632I 1927 CTCTTC CTCTTC(1/4)
MaeI 1138 CTAG C^TAG
MamI 1548 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 608 GGCGCC GG^CGCC
PaeR7I 562 CTCGAG C^TCGAG
PflFI 763 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1618 CACGTG CAC^GTG
PmlI 1618 CACGTG CAC^GTG
RsrII 479 CGGTCCG CG^G[AT]CCG
SapI 1926 GCTCTTC GCTCTTC(1/4)
ScaI 200 AGTACT AGT^ACT
SexAI 440 ACCTGGT A^CC[AT]GGT
SfoI 608 GGCGCC GGC^GCC
SphI 2171 GCATGC GCATG^C
SspBI 263 TGTACA T^GTACA
TfiI 8 GATTC G^A[AT]TC
Tsp509I 1276 AATT ^AATT
Tth111I 763 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 614 CCAAATGCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 562 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 752 GGTACC G^GTACC
1963 GGTACC
AhdI 427 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1261 GACCCTTCGTC
Asp718I 752 GGTACC G^GTACC
1963 GGTACC
AspEI 427 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1261 GACCCTTCGTC
BcgI 564 CGAGGGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
458 GCATCATCATCG
BglI 560 GCCTCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1894 GCCACAGAGGC
BsiEI 138 CGACCG CG[GA][CT]^CG
1476 CGGCCG
BsmAI 1118 GAGAC GTCTC(1/5)
1489 GAGAC
BspMI 284 ACCTGC ACCTGC(4/8)
1127 ACCTGC
BsrBI 1806 CCGCTC CCGCTC(-3/-3)
1770 GAGCGG
BsrDI 543 CATTGC GCAATG(2/0)
1056 CATTGC
BsrI 203 ACTGG ACTGG(1/-1)
510 CCAGT
BstEII 327 GGTGACC G^GT[ACGT]ACC
786 GGTTACC
BstPI 327 GGTGACC G^GT[ACGT]ACC
786 GGTTACC
DraIII 439 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2125 CACAAGGTG
Eam1105I 427 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1261 GACCCTTCGTC
EcoO65I 327 GGTGACC G^GT[ACGT]ACC
786 GGTTACC
HgaI 52 GACGC GACGC(5/10)
391 GACGC
KpnI 752 GGTACC GGTAC^C
1963 GGTACC
NcoI 1298 CCATGG C^CATGG
1382 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII