Restriction map for
pF1KB4735
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BanI BbeI
BbsI BbvCI BcgI BciVI BcnI
BglI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
DraI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI HapII Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PshAI PshBI Psp1406I
PstI PvuI SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 434 CGCG CG^CG
AflII 184 CTTAAG C^TTAAG
AflIII 909 ACACGT A^C[GA][CT]GT
AspHI 1096 GAGCAC G[AT]GC[AT]^C
AviII 974 TGCGCA TGC^GCA
BalI 311 TGGCCA TGG^CCA
BamHI 591 GGATCC G^GATCC
BbrPI 910 CACGTG CAC^GTG
BclI 948 TGATCA T^GATCA
BfaI 1090 CTAG C^TAG
BfrI 184 CTTAAG C^TTAAG
BglII 155 AGATCT A^GATCT
BsaAI 910 CACGTG [CT]AC^GT[GA]
BsiHKAI 1096 GAGCAC G[AT]GC[AT]^C
BsmBI 425 GAGACG CGTCTC(1/5)
BspHI 452 TCATGA T^CATGA
BspMI 838 ACCTGC ACCTGC(4/8)
BsrI 215 ACTGG ACTGG(1/-1)
BstAPI 555 GCACATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 434 CGCG CG^CG
BstXI 314 CCACGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 429 CGGTCCG CG^G[AT]CCG
DraIII 291 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 688 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 948 TGATCA T^GATCA
FspI 974 TGCGCA TGC^GCA
HaeII 399 GGCGCT [GA]GCGC^[CT]
HgaI 148 GACGC GACGC(5/10)
MaeI 1090 CTAG C^TAG
MluNI 311 TGGCCA TGG^CCA
MscI 311 TGGCCA TGG^CCA
MslI 1044 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 867 CAGCTG C[AC]G^C[GT]G
MvnI 434 CGCG CG^CG
NspI 677 GCATGC [GA]CATG^[CT]
PflMI 876 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 910 CACGTG CAC^GTG
PmlI 910 CACGTG CAC^GTG
PvuII 867 CAGCTG CAG^CTG
RcaI 452 TCATGA T^CATGA
RsrII 429 CGGTCCG CG^G[AT]CCG
SexAI 265 ACCTGGT A^CC[AT]GGT
SfcI 828 CTACAG C^T[GA][CT]AG
SphI 677 GCATGC GCATG^C
SspI 539 AATATT AAT^ATT
Van91I 876 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 592 GATCC GGATC(4/5)
591 GGATC
BanII 282 GAGCCC G[GA]GC[CT]^C
855 GAGCCC
BsgI 288 CTGCAC GTGCAG(16/14)
553 CTGCAC
Bsp1407I 298 TGTACA T^GTACA
494 TGTACA
BsrGI 298 TGTACA T^GTACA
494 TGTACA
BstYI 155 AGATCT [GA]^GATC[CT]
591 GGATCC
DdeI 577 CTCAG C^T[ACGT]AG
1111 CTGAG
EcoT14I 520 CCTTGG C^C[AT][AT]GG
628 CCTTGG
HphI 263 TCACC GGTGA(8/7)
845 TCACC
MflI 155 AGATCT [GA]^GATC[CT]
591 GGATCC
SmlI 132 CTCAAG C^T[CT][GA]AG
184 CTTAAG
SspBI 298 TGTACA T^GTACA
494 TGTACA
StyI 520 CCTTGG C^C[AT][AT]GG
628 CCTTGG
Tsp45I 245 GTGAC ^GT[GC]AC
262 GTCAC
XcmI 1074 CCAGCCCTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1104 CCAAGACCTGAGTGG
XhoII 155 AGATCT [GA]^GATC[CT]
591 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstEII BstF5I BstNI BstPI
Cac8I CfoI Cfr13I DpnII DraII
EaeI EcoNI EcoO109I EcoO65I EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI MaeII MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I