Restriction map for
pF1KB4718
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII AccIII AclI AcsI AflIII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HpaI Ksp632I
KspI MaeI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 581 GGTACC G^GTACC
AccI 181 GTCTAC GT^[AC][GT]AC
AccII 319 CGCG CG^CG
AciI 566 GCGG CCGC(-3/-1)
AflII 277 CTTAAG C^TTAAG
Alw44I 69 GTGCAC G^TGCAC
AlwI 123 GATCC GGATC(4/5)
AlwNI 301 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 69 GTGCAC G^TGCAC
Asp718I 581 GGTACC G^GTACC
AvaI 13 CTCGGG C^[CT]CG[GA]G
BbeI 58 GGCGCC GGCGC^C
BfrI 277 CTTAAG C^TTAAG
BsgI 235 GTGCAG GTGCAG(16/14)
BsoBI 13 CTCGGG C^[CT]CG[GA]G
Bsp1407I 338 TGTACA T^GTACA
BsrGI 338 TGTACA T^GTACA
BstEII 517 GGTTACC G^GT[ACGT]ACC
BstPI 517 GGTTACC G^GT[ACGT]ACC
BstUI 319 CGCG CG^CG
BstXI 131 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 517 GGTTACC G^GT[ACGT]ACC
HaeII 58 GGCGCC [GA]GCGC^[CT]
HincII 289 GTCAAC GT[CT]^[GA]AC
HindII 289 GTCAAC GT[CT]^[GA]AC
HindIII 254 AAGCTT A^AGCTT
HinfI 264 GAGTC G^A[ACGT]TC
KasI 58 GGCGCC G^GCGCC
KpnI 581 GGTACC GGTAC^C
MseI 278 TTAA T^TAA
MslI 219 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 319 CGCG CG^CG
NarI 58 GGCGCC GG^CGCC
PleI 264 GAGTC GAGTC(4/5)
PstI 573 CTGCAG CTGCA^G
SfcI 573 CTGCAG C^T[GA][CT]AG
SfoI 58 GGCGCC GGC^GCC
SmlI 277 CTTAAG C^T[CT][GA]AG
SspBI 338 TGTACA T^GTACA
Tru9I 278 TTAA T^TAA
Tsp45I 469 GTGAC ^GT[GC]AC
Tsp509I 511 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 58 GGCGCC G[GA]^CG[CT]C
97 GACGCC
ApaI 163 GGGCCC GGGCC^C
593 GGGCCC
AspHI 25 GTGCTC G[AT]GC[AT]^C
69 GTGCAC
AvaII 372 GGACC G^G[AT]CC
494 GGACC
BanI 58 GGCGCC G^G[CT][GA]CC
581 GGTACC
BbvI 35 GCAGC GCAGC(8/12)
575 GCAGC
BcnI 126 CCGGG CC^[GC]GG
600 CCCGG
BpmI 39 CTGGAG CTGGAG(16/14)
283 CTGGAG
BsaHI 58 GGCGCC G[GA]^CG[CT]C
97 GACGCC
BsiHKAI 25 GTGCTC G[AT]GC[AT]^C
69 GTGCAC
BsmAI 266 GTCTC GTCTC(1/5)
488 GTCTC
BsmFI 50 GGGAC GGGAC(10/14)
371 GGGAC
BspMI 305 ACCTGC ACCTGC(4/8)
455 ACCTGC
CfoI 59 GCGC GCG^C
393 GCGC
DpnII 123 GATC ^GATC
438 GATC
EcoT14I 7 CCTTGG C^C[AT][AT]GG
131 CCAAGG
HgaI 97 GACGC GACGC(5/10)
320 GCGTC
HhaI 59 GCGC GCG^C
393 GCGC
Hin1I 58 GGCGCC G[GA]^CG[CT]C
97 GACGCC
HinP1I 59 GCGC G^CGC
393 GCGC
MboI 123 GATC ^GATC
438 GATC
MboII 144 GAAGA GAAGA(8/7)
201 GAAGA
MspA1I 36 CAGCTG C[AC]G^C[GT]G
576 CAGCTG
NciI 126 CCGGG CC^[GC]GG
600 CCCGG
NdeII 123 GATC ^GATC
438 GATC
NspI 73 ACATGC [GA]CATG^[CT]
500 ACATGC
PvuII 36 CAGCTG CAG^CTG
576 CAGCTG
Sau3AI 123 GATC ^GATC
438 GATC
StyI 7 CCTTGG C^C[AT][AT]GG
131 CCAAGG
TseI 35 GCAGC G^C[AT]GC
575 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI BanII BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeIII
MnlI MspI MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI