Restriction map for
pF1KB4667
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BsaWI BseAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 1024 GACGTC GACGT^C
AccI 904 GTCTAC GT^[AC][GT]AC
AflIII 884 ACACGT A^C[GA][CT]GT
AocI 270 CCTCAGG CC^T[ACGT]AGG
Asp700I 561 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 635 GGATCC G^GATCC
BanI 118 GGCACC G^G[CT][GA]CC
BglII 956 AGATCT A^GATCT
BlpI 681 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 681 GCTCAGC GC^T[ACGT]AGC
BsaAI 885 CACGTA [CT]AC^GT[GA]
BsaBI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 946 GAGACC GGTCTC(1/5)
BseRI 892 GAGGAG GAGGAG(10/8)
BsgI 313 GTGCAG GTGCAG(16/14)
BsiEI 294 CGACCG CG[GA][CT]^CG
BsiWI 887 CGTACG C^GTACG
BsmAI 946 GAGAC GTCTC(1/5)
Bsu36I 270 CCTCAGG CC^T[ACGT]AGG
CelII 681 GCTCAGC GC^T[ACGT]AGC
DsaI 545 CCACGG C^C[GA][CT]GG
EaeI 263 TGGCCG [CT]^GGCC[GA]
EarI 799 CTCTTC CTCTTC(1/4)
Eco81I 270 CCTCAGG CC^T[ACGT]AGG
EcoT14I 950 CCAAGG C^C[AT][AT]GG
Ksp632I 799 CTCTTC CTCTTC(1/4)
MamI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PpuMI 815 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1020 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 494 CAGCTG CAG^CTG
SmaI 533 CCCGGG CCC^GGG
SmlI 1045 CTCAAG C^T[CT][GA]AG
StyI 950 CCAAGG C^C[AT][AT]GG
Tsp509I 54 AATT ^AATT
XcmI 545 CCACGGGCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 533 CCCGGG C^CCGGG
XmaI 533 CCCGGG C^CCGGG
XmnI 561 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 652 GGCGTC G[GA]^CG[CT]C
1024 GACGTC
ApaI 257 GGGCCC GGGCC^C
333 GGGCCC
AvaII 44 GGTCC G^G[AT]CC
816 GGACC
BbsI 925 GAAGAC GAAGAC(2/6)
1006 GTCTTC
BpuAI 925 GAAGAC GAAGAC(2/6)
1006 GTCTTC
BsaHI 652 GGCGTC G[GA]^CG[CT]C
1024 GACGTC
BsmFI 536 GGGAC GGGAC(10/14)
45 GTCCC
BsrBI 426 CCGCTC CCGCTC(-3/-3)
847 CCGCTC
HgaI 1012 GACGC GACGC(5/10)
653 GCGTC
Hin1I 652 GGCGTC G[GA]^CG[CT]C
1024 GACGTC
HinfI 235 GACTC G^A[ACGT]TC
754 GACTC
NlaIII 381 CATG CATG^
540 CATG
PleI 235 GACTC GAGTC(4/5)
754 GACTC
SacI 580 GAGCTC GAGCT^C
679 GAGCTC
StuI 191 AGGCCT AGG^CCT
413 AGGCCT
Tsp45I 655 GTCAC ^GT[GC]AC
1018 GTGAC
TspRI 510 CACTG CA[GC]TG[ACGT][ACGT]^
640 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TthHB8I
XhoII