Restriction map for
pF1KB4662
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BfrI BlnI BlpI
BmyI Bpu1102I BsaBI BsaHI BsaI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoT14I EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AlwNI 691 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 631 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 280 GGACC G^G[AT]CC
BanI 40 GGCACC G^G[CT][GA]CC
BbvI 862 GCAGC GCAGC(8/12)
BclI 809 TGATCA T^GATCA
BcnI 44 CCGGG CC^[GC]GG
BglI 415 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 880 AGATCT A^GATCT
BsaAI 664 TACGTG [CT]AC^GT[GA]
BsaWI 400 TCCGGT [AT]^CCGG[AT]
BsgI 1073 GTGCAG GTGCAG(16/14)
BsmAI 540 GAGAC GTCTC(1/5)
BsmFI 266 GGGAC GGGAC(10/14)
BspHI 1091 TCATGA T^CATGA
Cfr13I 280 GGACC G^G[ACGT]CC
DraIII 898 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 886 GAATTC G^AATTC
FauI 1043 CCCGC CCCGC(4/6)
FbaI 809 TGATCA T^GATCA
Fnu4HI 862 GCAGC GC^[ACGT]GC
HincII 208 GTTAAC GT[CT]^[GA]AC
HindII 208 GTTAAC GT[CT]^[GA]AC
HindIII 788 AAGCTT A^AGCTT
HpaI 208 GTTAAC GTT^AAC
ItaI 862 GCAGC GC^[ACGT]GC
MslI 132 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 863 CAGCTG C[AC]G^C[GT]G
NciI 44 CCGGG CC^[GC]GG
NlaIV 40 GGCACC GG[ACGT]^[ACGT]CC
PvuII 863 CAGCTG CAG^CTG
RcaI 1091 TCATGA T^CATGA
Sau96I 280 GGACC G^G[ACGT]CC
SmlI 443 CTCAAG C^T[CT][GA]AG
SspI 676 AATATT AAT^ATT
TaqI 1179 TCGA T^CGA
TfiI 724 GAATC G^A[AT]TC
TseI 862 GCAGC G^C[AT]GC
TthHB8I 1179 TCGA T^CGA
XmnI 631 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 469 GTAGAC GT^[AC][GT]AC
918 GTCTAC
AcsI 886 GAATTC [GA]^AATT[CT]
1015 AAATTT
AlwI 1023 GATCC GGATC(4/5)
159 GGATC
ApoI 886 GAATTC [GA]^AATT[CT]
1015 AAATTT
BbsI 110 GAAGAC GAAGAC(2/6)
1033 GAAGAC
BciVI 601 GGATAC GTATCC(6/5)
927 GTATCC
BpmI 476 CTGGAG CTGGAG(16/14)
911 CTGGAG
BpuAI 110 GAAGAC GAAGAC(2/6)
1033 GAAGAC
BsaJI 9 CCAGGG C^C[ACGT][ACGT]GG
1065 CCTGGG
BsiYI 96 CCCAGCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1134 CCAAGAAAAGG
BslI 96 CCCAGCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1134 CCAAGAAAAGG
BstXI 131 CCACCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
536 CCAGGAGACTGG
Cac8I 67 GCAGGC GC[ACGT]^[ACGT]GC
935 GCCTGC
DdeI 702 CTTAG C^T[ACGT]AG
975 CTTAG
EcoRV 572 GATATC GAT^ATC
1087 GATATC
HaeIII 699 GGCC GG^CC
934 GGCC
HapII 44 CCGG C^CGG
401 CCGG
HpaII 44 CCGG C^CGG
401 CCGG
HphI 172 GGTGA GGTGA(8/7)
994 GGTGA
MaeII 665 ACGT A^CGT
747 ACGT
MspI 44 CCGG C^CGG
401 CCGG
PleI 472 GACTC GAGTC(4/5)
489 GACTC
Tsp45I 487 GTGAC ^GT[GC]AC
1171 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI BfaI BsrI
BssKI BstF5I BstNI BstYI DpnII
EcoRII FokI HinfI MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI ScrFI Tru9I Tsp509I XhoII