Restriction map for
pF1KB4609
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI ApaI
ApoI AscI Asp718I AvaI AviII
AvrII BbrPI BbsI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BseRI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EcoRI
EcoRV FauI FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AcyI 406 GGCGCC G[GA]^CG[CT]C
AhdI 44 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 364 CCTCAGG CC^T[ACGT]AGG
AseI 268 ATTAAT AT^TAAT
AsnI 268 ATTAAT AT^TAAT
Asp700I 420 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 44 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 724 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 155 TGGCCA TGG^CCA
BamHI 198 GGATCC G^GATCC
BanI 406 GGCGCC G^G[CT][GA]CC
BanII 133 GAGCTC G[GA]GC[CT]^C
BbeI 406 GGCGCC GGCGC^C
BbvCI 215 CCTCAGC CCTCAGC(-5/-2)
BcgI 776 GCAACGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 211 GTATCC GTATCC(6/5)
BcnI 403 CCCGG CC^[GC]GG
BsaAI 616 TACGTG [CT]AC^GT[GA]
BsaHI 406 GGCGCC G[GA]^CG[CT]C
BsgI 460 CTGCAC GTGCAG(16/14)
BsiWI 614 CGTACG C^GTACG
BsmAI 610 GAGAC GTCTC(1/5)
BsmBI 610 GAGACG CGTCTC(1/5)
BsrFI 538 ACCGGC [GA]^CCGG[CT]
BsrI 543 CCAGT ACTGG(1/-1)
BstAPI 621 GCACATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 493 TTCGAA TT^CGAA
BstEII 51 GGTCACC G^GT[ACGT]ACC
BstPI 51 GGTCACC G^GT[ACGT]ACC
Bsu36I 364 CCTCAGG CC^T[ACGT]AGG
Cfr10I 538 ACCGGC [GA]^CCGG[CT]
DsaI 56 CCATGG C^C[GA][CT]GG
EagI 231 CGGCCG C^GGCCG
Eam1105I 44 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 231 CGGCCG C^GGCCG
Eco52I 231 CGGCCG C^GGCCG
Eco81I 364 CCTCAGG CC^T[ACGT]AGG
EcoNI 813 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 51 GGTCACC G^GT[ACGT]ACC
EcoT22I 628 ATGCAT ATGCA^T
Hin1I 406 GGCGCC G[GA]^CG[CT]C
KasI 406 GGCGCC G^GCGCC
MluNI 155 TGGCCA TGG^CCA
MscI 155 TGGCCA TGG^CCA
MseI 269 TTAA T^TAA
MslI 54 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 406 GGCGCC GG^CGCC
NciI 403 CCCGG CC^[GC]GG
NcoI 56 CCATGG C^CATGG
NsiI 628 ATGCAT ATGCA^T
NspV 493 TTCGAA TT^CGAA
PflFI 724 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 268 ATTAAT AT^TAAT
PvuII 594 CAGCTG CAG^CTG
SacI 133 GAGCTC GAGCT^C
SfcI 771 CTACAG C^T[GA][CT]AG
SfiI 307 GGCCCAATGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 406 GGCGCC GGC^GCC
SfuI 493 TTCGAA TT^CGAA
Tru9I 269 TTAA T^TAA
Tsp509I 601 AATT ^AATT
Tth111I 724 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 348 CCAAGGGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 420 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 589 GTGCAC G^TGCAC
619 GTGCAC
AlwNI 159 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
481 CAGACGCTG
Aor51HI 90 AGCGCT AGC^GCT
422 AGCGCT
ApaLI 589 GTGCAC G^TGCAC
619 GTGCAC
BglI 308 GCCCAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
362 GCCCTCAGGGC
BsiEI 231 CGGCCG CG[GA][CT]^CG
536 CGACCG
BstF5I 279 CATCC GGATG(2/0)
651 CATCC
BstYI 119 AGATCC [GA]^GATC[CT]
198 GGATCC
Eco47III 90 AGCGCT AGC^GCT
422 AGCGCT
EcoT14I 56 CCATGG C^C[AT][AT]GG
348 CCAAGG
FokI 279 CATCC GGATG(9/13)
651 CATCC
HgaI 483 GACGC GACGC(5/10)
728 GCGTC
HinfI 22 GAGTC G^A[ACGT]TC
181 GACTC
MaeII 613 ACGT A^CGT
617 ACGT
MflI 119 AGATCC [GA]^GATC[CT]
198 GGATCC
PleI 181 GACTC GAGTC(4/5)
22 GAGTC
SapI 489 GCTCTTC GCTCTTC(1/4)
741 GCTCTTC
StyI 56 CCATGG C^C[AT][AT]GG
348 CCAAGG
TaqI 494 TCGA T^CGA
785 TCGA
TspRI 4 CAGTG CA[GC]TG[ACGT][ACGT]^
440 CAGTG
TthHB8I 494 TCGA T^CGA
785 TCGA
XhoII 119 AGATCC [GA]^GATC[CT]
198 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I