Restriction map for
pF1KB3747
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BbvCI BciVI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BsgI BsiWI BsmAI BsmBI BsmI
BspDI BspEI BspHI BspMI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HindIII
HpaI KasI KpnI MaeI MamI
MfeI MluI MroI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 60 GAATTT [GA]^AATT[CT]
AflIII 371 ACATGT A^C[GA][CT]GT
AhdI 463 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 60 GAATTT [GA]^AATT[CT]
AspEI 463 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 294 GAGCTC G[AT]GC[AT]^C
AvaI 291 CCCGAG C^[CT]CG[GA]G
BalI 899 TGGCCA TGG^CCA
BamHI 773 GGATCC G^GATCC
BanI 802 GGTGCC G^G[CT][GA]CC
BcgI 580 GCACTCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 305 TGATCA T^GATCA
BpmI 256 CTCCAG CTGGAG(16/14)
BsaAI 202 TACGTG [CT]AC^GT[GA]
BsaWI 455 TCCGGT [AT]^CCGG[AT]
BsiEI 469 CGGTCG CG[GA][CT]^CG
BsiHKAI 294 GAGCTC G[AT]GC[AT]^C
BsoBI 291 CCCGAG C^[CT]CG[GA]G
Bsp1407I 323 TGTACA T^GTACA
BspLU11I 371 ACATGT A^CATGT
BsrBI 684 GAGCGG CCGCTC(-3/-3)
BsrGI 323 TGTACA T^GTACA
BssSI 412 CTCGTG CACGAG(-5/-1)
BstYI 773 GGATCC [GA]^GATC[CT]
Eam1105I 463 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 481 GAAGAG CTCTTC(1/4)
EcoNI 750 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 665 CCATGG C^C[AT][AT]GG
FbaI 305 TGATCA T^GATCA
Ksp632I 481 GAAGAG CTCTTC(1/4)
KspI 340 CCGCGG CCGC^GG
MflI 773 GGATCC [GA]^GATC[CT]
MluNI 899 TGGCCA TGG^CCA
MscI 899 TGGCCA TGG^CCA
MseI 272 TTAA T^TAA
NcoI 665 CCATGG C^CATGG
NspI 371 ACATGT [GA]CATG^[CT]
PpuMI 407 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 36 CTGCAG CTGCA^G
PvuII 592 CAGCTG CAG^CTG
SacI 294 GAGCTC GAGCT^C
SacII 340 CCGCGG CCGC^GG
SmlI 249 CTTGAG C^T[CT][GA]AG
SspBI 323 TGTACA T^GTACA
StyI 665 CCATGG C^C[AT][AT]GG
TaqI 674 TCGA T^CGA
Tru9I 272 TTAA T^TAA
TspRI 539 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 674 TCGA T^CGA
XhoII 773 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 324 GTAC GT^AC
597 GTAC
AvaII 344 GGACC G^G[AT]CC
408 GGTCC
BglI 892 GCCGCGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
911 GCCTGAACGGC
BseRI 583 CTCCTC GAGGAG(10/8)
720 CTCCTC
BsmFI 932 GGGAC GGGAC(10/14)
969 GTCCC
BsrI 258 CCAGT ACTGG(1/-1)
494 CCAGT
CfoI 610 GCGC GCG^C
643 GCGC
DraII 407 GGGTCCT [GA]G^G[ACGT]CC[CT]
624 AGGCCCC
DsaI 340 CCGCGG C^C[GA][CT]GG
665 CCATGG
EaeI 26 TGGCCG [CT]^GGCC[GA]
899 TGGCCA
EcoO109I 407 GGGTCCT [GA]G^G[ACGT]CC[CT]
624 AGGCCCC
HapII 456 CCGG C^CGG
725 CCGG
HhaI 610 GCGC GCG^C
643 GCGC
HinP1I 610 GCGC G^CGC
643 GCGC
HincII 70 GTCAAC GT[CT]^[GA]AC
700 GTCAAC
HindII 70 GTCAAC GT[CT]^[GA]AC
700 GTCAAC
HinfI 139 GAATC G^A[ACGT]TC
650 GATTC
HpaII 456 CCGG C^CGG
725 CCGG
MspI 456 CCGG C^CGG
725 CCGG
RsaI 324 GTAC GT^AC
597 GTAC
SfaNI 217 GATGC GCATC(5/9)
760 GATGC
SfcI 36 CTGCAG C^T[GA][CT]AG
425 CTACAG
TfiI 139 GAATC G^A[AT]TC
650 GATTC
Tsp509I 61 AATT ^AATT
489 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
BanII BbsI BbvI BmyI BpuAI
BsaHI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HgaI Hin1I
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MvaI
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI Tsp45I