Restriction map for
pF1KB3711
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# NO CUT ENZYMES ( 195 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DdeI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HpaII HphI KasI KpnI
Ksp632I MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 215 GTAC GT^AC
AlwI 154 GATCC GGATC(4/5)
AlwNI 170 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 201 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 88 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 117 GGACC G^G[AT]CC
BbrPI 142 CACGTG CAC^GTG
BfaI 32 CTAG C^TAG
BsaAI 142 CACGTG [CT]AC^GT[GA]
BsaI 99 GGTCTC GGTCTC(1/5)
BsiYI 41 CCCAAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 41 CCCAAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 100 GTCTC GTCTC(1/5)
BsrI 169 CCAGT ACTGG(1/-1)
BstYI 153 AGATCC [GA]^GATC[CT]
Cac8I 174 GCCTGC GC[ACGT]^[ACGT]GC
Cfr13I 117 GGACC G^G[ACGT]CC
DpnII 154 GATC ^GATC
DsaI 113 CCGCGG C^C[GA][CT]GG
FauI 147 GCGGG CCCGC(4/6)
HaeIII 126 GGCC GG^CC
KspI 113 CCGCGG CCGC^GG
MaeI 32 CTAG C^TAG
MaeII 143 ACGT A^CGT
MboI 154 GATC ^GATC
MflI 153 AGATCC [GA]^GATC[CT]
MslI 165 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 113 CCGCGG C[AC]G^C[GT]G
NdeII 154 GATC ^GATC
NlaIII 12 CATG CATG^
NruI 195 TCGCGA TCG^CGA
NspI 11 GCATGT [GA]CATG^[CT]
PflFI 88 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 142 CACGTG CAC^GTG
PmlI 142 CACGTG CAC^GTG
RsaI 215 GTAC GT^AC
SacII 113 CCGCGG CCGC^GG
Sau3AI 154 GATC ^GATC
Sau96I 117 GGACC G^G[ACGT]CC
TspRI 170 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 88 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 47 CCAGGAAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 153 AGATCC [GA]^GATC[CT]
XmnI 201 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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AccII 114 CGCG CG^CG
196 CGCG
BbvI 63 GCAGC GCAGC(8/12)
109 GCAGC
BsaJI 104 CCCAGG C^C[ACGT][ACGT]GG
113 CCGCGG
BstUI 114 CGCG CG^CG
196 CGCG
MboII 151 GAAGA GAAGA(8/7)
201 GAAGA
MvnI 114 CGCG CG^CG
196 CGCG
MwoI 26 GCACCACTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
46 GCCAGGAAAGC
SfaNI 35 GCATC GCATC(5/9)
178 GCATC
TseI 63 GCAGC G^C[AT]GC
109 GCAGC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AciI AluI BssKI BstNI EcoRII
Fnu4HI ItaI MnlI MvaI ScrFI