Restriction map for
pF1KB3490
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BpmI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KpnI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 703 GTCTAC GT^[AC][GT]AC
AccII 36 CGCG CG^CG
AflIII 561 ACATGT A^C[GA][CT]GT
BbsI 148 GAAGAC GAAGAC(2/6)
BbvCI 8 CCTCAGC CCTCAGC(-5/-2)
BcnI 716 CCGGG CC^[GC]GG
BlpI 954 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 954 GCTGAGC GC^T[ACGT]AGC
BpuAI 148 GAAGAC GAAGAC(2/6)
BseRI 694 GAGGAG GAGGAG(10/8)
BspLU11I 561 ACATGT A^CATGT
BsrBI 724 CCGCTC CCGCTC(-3/-3)
BsrFI 77 ACCGGC [GA]^CCGG[CT]
BssSI 507 CACGAG CACGAG(-5/-1)
BstBI 881 TTCGAA TT^CGAA
BstUI 36 CGCG CG^CG
BstYI 947 AGATCC [GA]^GATC[CT]
CelII 954 GCTGAGC GC^T[ACGT]AGC
Cfr10I 77 ACCGGC [GA]^CCGG[CT]
EaeI 79 CGGCCA [CT]^GGCC[GA]
EcoRV 826 GATATC GAT^ATC
EcoT14I 254 CCATGG C^C[AT][AT]GG
HincII 862 GTTGAC GT[CT]^[GA]AC
HindII 862 GTTGAC GT[CT]^[GA]AC
KspI 35 CCGCGG CCGC^GG
MflI 947 AGATCC [GA]^GATC[CT]
MslI 666 CACCAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 36 CGCG CG^CG
NciI 716 CCGGG CC^[GC]GG
NcoI 254 CCATGG C^CATGG
NheI 325 GCTAGC G^CTAGC
NspV 881 TTCGAA TT^CGAA
PvuII 952 CAGCTG CAG^CTG
SacI 957 GAGCTC GAGCT^C
SacII 35 CCGCGG CCGC^GG
SfcI 786 CTACAG C^T[GA][CT]AG
SfuI 881 TTCGAA TT^CGAA
SpeI 612 ACTAGT A^CTAGT
SspI 339 AATATT AAT^ATT
StyI 254 CCATGG C^C[AT][AT]GG
XhoII 947 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 36 enzymes) #
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AcsI 244 GAATTT [GA]^AATT[CT]
430 AAATTC
AcyI 382 GACGCC G[GA]^CG[CT]C
397 GACGCC
AfaI 125 GTAC GT^AC
918 GTAC
AlwI 312 GATCC GGATC(4/5)
948 GATCC
AlwNI 85 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
447 CAGATTCTG
ApoI 244 GAATTT [GA]^AATT[CT]
430 AAATTC
AspHI 663 GAGCAC G[AT]GC[AT]^C
957 GAGCTC
BanI 719 GGCACC G^G[CT][GA]CC
934 GGCACC
BanII 644 GGGCTC G[GA]GC[CT]^C
957 GAGCTC
BsaHI 382 GACGCC G[GA]^CG[CT]C
397 GACGCC
BsaI 4 GAGACC GGTCTC(1/5)
989 GAGACC
BsgI 504 CTGCAC GTGCAG(16/14)
711 CTGCAC
BsiHKAI 663 GAGCAC G[AT]GC[AT]^C
957 GAGCTC
BsmI 270 GAATGC GAATGC(1/-1)
405 GAATGC
BspMI 56 ACCTGC ACCTGC(4/8)
709 ACCTGC
BsrDI 738 CATTGC GCAATG(2/0)
966 CATTGC
BsrI 755 ACTGG ACTGG(1/-1)
626 CCAGT
BstXI 254 CCATGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
743 CCAACATCCTGG
Cac8I 33 GCCCGC GC[ACGT]^[ACGT]GC
325 GCTAGC
DsaI 35 CCGCGG C^C[GA][CT]GG
254 CCATGG
EarI 416 GAAGAG CTCTTC(1/4)
574 GAAGAG
HapII 78 CCGG C^CGG
716 CCGG
HgaI 382 GACGC GACGC(5/10)
397 GACGC
Hin1I 382 GACGCC G[GA]^CG[CT]C
397 GACGCC
HpaII 78 CCGG C^CGG
716 CCGG
Ksp632I 416 GAAGAG CTCTTC(1/4)
574 GAAGAG
MseI 344 TTAA T^TAA
356 TTAA
MspI 78 CCGG C^CGG
716 CCGG
NspI 561 ACATGT [GA]CATG^[CT]
635 ACATGC
PleI 390 GACTC GAGTC(4/5)
531 GACTC
RsaI 125 GTAC GT^AC
918 GTAC
TaqI 303 TCGA T^CGA
882 TCGA
TfiI 449 GATTC G^A[AT]TC
765 GAATC
Tru9I 344 TTAA T^TAA
356 TTAA
TspRI 627 CAGTG CA[GC]TG[ACGT][ACGT]^
818 CACTG
TthHB8I 303 TCGA T^CGA
882 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AvaII BbvI BfaI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SmlI TseI Tsp45I Tsp509I