##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AcyI        AfaI        AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BglI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Cac8I       CfoI        Cfr10I      
ClaI        CpoI        DpnII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        KpnI        
Ksp632I     KspI        MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        TaqI        TfiI        TseI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AclI          310  AACGTT               AA^CGTT
AflII          81  CTTAAG               C^TTAAG
AocI          185  CCTCAGG              CC^T[ACGT]AGG
ApaI          481  GGGCCC               GGGCC^C
AseI          141  ATTAAT               AT^TAAT
AsnI          141  ATTAAT               AT^TAAT
AvaII         182  GGTCC                G^G[AT]CC
BanI          238  GGTGCC               G^G[CT][GA]CC
BanII         481  GGGCCC               G[GA]GC[CT]^C
BfrI           81  CTTAAG               C^TTAAG
BlpI          248  GCTTAGC              GC^T[ACGT]AGC
BmyI          481  GGGCCC               G[AGT]GC[ACT]^C
BpmI          206  CTGGAG               CTGGAG(16/14)
Bpu1102I      248  GCTTAGC              GC^T[ACGT]AGC
Bsp1286I      481  GGGCCC               G[AGT]GC[ACT]^C
Bsu36I        185  CCTCAGG              CC^T[ACGT]AGG
CelII         248  GCTTAGC              GC^T[ACGT]AGC
DraI           75  TTTAAA               TTT^AAA
DraII         480  AGGGCCC              [GA]G^G[ACGT]CC[CT]
Eco81I        185  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      480  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         355  GAATTC               G^AATTC
HaeIII        482  GGCC                 GG^CC
HgaI          245  GACGC                GACGC(5/10)
HincII        307  GTTAAC               GT[CT]^[GA]AC
HindII        307  GTTAAC               GT[CT]^[GA]AC
HindIII       148  AAGCTT               A^AGCTT
HpaI          307  GTTAAC               GTT^AAC
NspI          329  ACATGC               [GA]CATG^[CT]
PshBI         141  ATTAAT               AT^TAAT
Psp1406I      310  AACGTT               AA^CGTT
PstI          262  CTGCAG               CTGCA^G
SfaNI           4  GATGC                GCATC(5/9)
SfcI          262  CTGCAG               C^T[GA][CT]AG
SmlI           81  CTTAAG               C^T[CT][GA]AG
SspI          285  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BfaI          161  CTAG                 C^TAG
              271  CTAG                
BsrI          205  ACTGG                ACTGG(1/-1)
              383  CCAGT               
BstF5I        403  CATCC                GGATG(2/0)
                3  GGATG               
FokI          403  CATCC                GGATG(9/13)
                3  GGATG               
MaeI          161  CTAG                 C^TAG
              271  CTAG                
MaeII         123  ACGT                 A^CGT
              311  ACGT                
MnlI          185  CCTC                 CCTC(7/6)
              376  CCTC                
NlaIII        330  CATG                 CATG^
              379  CATG                
NlaIV         238  GGTGCC               GG[ACGT]^[ACGT]CC
              481  GGGCCC              


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AcsI        AluI        ApoI        Cfr13I      DdeI        
MaeIII      MboII       MseI        Sau96I      Tru9I       
Tsp509I     TspRI