Restriction map for
pF1KB3385
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII AccI AclI AcsI AcyI
AflII AflIII AgeI AhdI AlwNI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseRI BsgI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI HphI KasI KspI
MaeI MamI MfeI MluI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 282 GGTACC G^GTACC
AccII 310 CGCG CG^CG
AccIII 200 TCCGGA T^CCGGA
Alw44I 302 GTGCAC G^TGCAC
ApaI 323 GGGCCC GGGCC^C
ApaLI 302 GTGCAC G^TGCAC
Asp718I 282 GGTACC G^GTACC
AspHI 302 GTGCAC G[AT]GC[AT]^C
BalI 104 TGGCCA TGG^CCA
BanI 282 GGTACC G^G[CT][GA]CC
BbvI 329 GCAGC GCAGC(8/12)
BseAI 200 TCCGGA T^CCGGA
BsiHKAI 302 GTGCAC G[AT]GC[AT]^C
BspEI 200 TCCGGA T^CCGGA
BsrBI 501 CCGCTC CCGCTC(-3/-3)
BstUI 310 CGCG CG^CG
BstYI 356 AGATCC [GA]^GATC[CT]
DraII 461 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 239 CCGTGG C^C[GA][CT]GG
EarI 144 GAAGAG CTCTTC(1/4)
EcoO109I 461 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 64 GATATC GAT^ATC
EcoT14I 506 CCAAGG C^C[AT][AT]GG
HindIII 211 AAGCTT A^AGCTT
KpnI 282 GGTACC GGTAC^C
Ksp632I 144 GAAGAG CTCTTC(1/4)
MaeII 497 ACGT A^CGT
MaeIII 313 GTGAC ^GT[ACGT]AC
MflI 356 AGATCC [GA]^GATC[CT]
MluNI 104 TGGCCA TGG^CCA
MroI 200 TCCGGA T^CCGGA
MscI 104 TGGCCA TGG^CCA
MspA1I 373 CAGCTG C[AC]G^C[GT]G
MvnI 310 CGCG CG^CG
PvuII 373 CAGCTG CAG^CTG
SexAI 131 ACCAGGT A^CC[AT]GGT
SfaNI 335 GCATC GCATC(5/9)
StyI 506 CCAAGG C^C[AT][AT]GG
TseI 329 GCAGC G^C[AT]GC
Tsp45I 313 GTGAC ^GT[GC]AC
XhoII 356 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 283 GTAC GT^AC
444 GTAC
AluI 212 AGCT AG^CT
374 AGCT
AlwI 198 GATCC GGATC(4/5)
357 GATCC
BanII 49 GAGCCC G[GA]GC[CT]^C
323 GGGCCC
BsaWI 200 TCCGGA [AT]^CCGG[AT]
418 TCCGGT
BsiEI 172 CGGCCG CG[GA][CT]^CG
236 CGGCCG
BsmFI 250 GGGAC GGGAC(10/14)
183 GTCCC
BsrFI 170 ACCGGC [GA]^CCGG[CT]
446 ACCGGC
CfoI 42 GCGC GCG^C
309 GCGC
Cfr10I 170 ACCGGC [GA]^CCGG[CT]
446 ACCGGC
DpnII 198 GATC ^GATC
357 GATC
EagI 172 CGGCCG C^GGCCG
236 CGGCCG
EclXI 172 CGGCCG C^GGCCG
236 CGGCCG
Eco52I 172 CGGCCG C^GGCCG
236 CGGCCG
HhaI 42 GCGC GCG^C
309 GCGC
HinP1I 42 GCGC G^CGC
309 GCGC
HinfI 159 GAGTC G^A[ACGT]TC
385 GACTC
MboI 198 GATC ^GATC
357 GATC
NdeII 198 GATC ^GATC
357 GATC
PleI 385 GACTC GAGTC(4/5)
159 GAGTC
RsaI 283 GTAC GT^AC
444 GTAC
Sau3AI 198 GATC ^GATC
357 GATC
TaqI 368 TCGA T^CGA
521 TCGA
TspRI 305 CACTG CA[GC]TG[ACGT][ACGT]^
405 CACTG
TthHB8I 368 TCGA T^CGA
521 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AocI AvaII BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Bsu36I Cac8I Cfr13I
DdeI EaeI Eco81I EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboII MnlI MspI MvaI
MwoI NciI NlaIV Sau96I ScrFI