Restriction map for
pF1KB3111
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AlwNI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BseAI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV EcoT14I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 281 GTAGAC GT^[AC][GT]AC
AccII 118 CGCG CG^CG
AciI 429 GCGG CCGC(-3/-1)
AgeI 7 ACCGGT A^CCGGT
AhdI 284 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 131 GTGCAC G^TGCAC
AocI 297 CCTGAGG CC^T[ACGT]AGG
ApaLI 131 GTGCAC G^TGCAC
AspEI 284 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 131 GTGCAC G[AT]GC[AT]^C
BanI 907 GGCGCC G^G[CT][GA]CC
BanII 383 GGGCTC G[GA]GC[CT]^C
BbeI 907 GGCGCC GGCGC^C
BcgI 77 GCAGTATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 221 TGATCA T^GATCA
BcnI 288 CCCGG CC^[GC]GG
BglII 482 AGATCT A^GATCT
BsaAI 134 CACGTA [CT]AC^GT[GA]
BseRI 121 GAGGAG GAGGAG(10/8)
BsgI 724 CTGCAC GTGCAG(16/14)
BsiHKAI 131 GTGCAC G[AT]GC[AT]^C
BsmBI 1060 GAGACG CGTCTC(1/5)
BsmI 449 GCATTC GAATGC(1/-1)
BspHI 855 TCATGA T^CATGA
BsrBI 427 GAGCGG CCGCTC(-3/-3)
BsrDI 736 GCAATG GCAATG(2/0)
BstUI 118 CGCG CG^CG
Bsu36I 297 CCTGAGG CC^T[ACGT]AGG
Eam1105I 284 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 238 GAAGAG CTCTTC(1/4)
Eco81I 297 CCTGAGG CC^T[ACGT]AGG
FauI 429 GCGGG CCCGC(4/6)
FbaI 221 TGATCA T^GATCA
HaeII 907 GGCGCC [GA]GCGC^[CT]
KasI 907 GGCGCC G^GCGCC
Ksp632I 238 GAAGAG CTCTTC(1/4)
MaeII 135 ACGT A^CGT
MslI 6 CACCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 272 CAGCTG C[AC]G^C[GT]G
MvnI 118 CGCG CG^CG
NarI 907 GGCGCC GG^CGCC
NciI 288 CCCGG CC^[GC]GG
PinAI 7 ACCGGT A^CCGGT
PleI 502 GAGTC GAGTC(4/5)
PvuII 272 CAGCTG CAG^CTG
RcaI 855 TCATGA T^CATGA
SfoI 907 GGCGCC GGC^GCC
SgrAI 6 CACCGGTG C[GA]^CCGG[CT]G
StuI 671 AGGCCT AGG^CCT
XbaI 666 TCTAGA T^CTAGA
XcmI 1013 CCAGCATTAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 899 GGCGTC G[GA]^CG[CT]C
907 GGCGCC
BfaI 317 CTAG C^TAG
667 CTAG
BmyI 131 GTGCAC G[AGT]GC[ACT]^C
383 GGGCTC
BpmI 803 CTCCAG CTGGAG(16/14)
652 CTGGAG
BsaHI 899 GGCGTC G[GA]^CG[CT]C
907 GGCGCC
BsaJI 287 CCCCGG C^C[ACGT][ACGT]GG
1055 CCTCGG
BsmAI 1060 GAGAC GTCTC(1/5)
747 GTCTC
Bsp1286I 131 GTGCAC G[AGT]GC[ACT]^C
383 GGGCTC
BspMI 568 GCAGGT ACCTGC(4/8)
987 GCAGGT
BsrFI 7 ACCGGT [GA]^CCGG[CT]
533 ACCGGC
BstEII 291 GGTCACC G^GT[ACGT]ACC
990 GGTTACC
BstPI 291 GGTCACC G^GT[ACGT]ACC
990 GGTTACC
Cfr10I 7 ACCGGT [GA]^CCGG[CT]
533 ACCGGC
EcoO65I 291 GGTCACC G^GT[ACGT]ACC
990 GGTTACC
EcoT22I 58 ATGCAT ATGCA^T
833 ATGCAT
HgaI 762 GACGC GACGC(5/10)
900 GCGTC
Hin1I 899 GGCGTC G[GA]^CG[CT]C
907 GGCGCC
MaeI 317 CTAG C^TAG
667 CTAG
MseI 28 TTAA T^TAA
107 TTAA
NsiI 58 ATGCAT ATGCA^T
833 ATGCAT
Tru9I 28 TTAA T^TAA
107 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbvI
BsaWI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI TseI Tsp45I Tsp509I
TspRI XhoII