Restriction map for
pF1KB0170
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcnI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstEII BstPI BstUI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FauI FseI FspI HaeII HapII
HgaI HhaI HinP1I HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacII SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 336 GACGTC GACGT^C
AciI 1785 CCGC CCGC(-3/-1)
AcyI 336 GACGTC G[GA]^CG[CT]C
AflIII 1970 ACATGT A^C[GA][CT]GT
Alw44I 1925 GTGCAC G^TGCAC
AlwNI 406 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1925 GTGCAC G^TGCAC
Asp700I 2538 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 1787 GCAGGCATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1338 GGATAC GTATCC(6/5)
BpmI 2767 CTCCAG CTGGAG(16/14)
BsaHI 336 GACGTC G[GA]^CG[CT]C
BsgI 978 CTGCAC GTGCAG(16/14)
BspHI 1331 TCATGA T^CATGA
BspLU11I 1970 ACATGT A^CATGT
BspMI 3076 GCAGGT ACCTGC(4/8)
Bst1107I 2158 GTATAC GTA^TAC
BstZ17I 2158 GTATAC GTA^TAC
DraII 1046 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 108 CCATGG C^C[GA][CT]GG
EcoNI 3123 CCTACGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1046 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 70 GATATC GAT^ATC
Hin1I 336 GACGTC G[GA]^CG[CT]C
HindIII 1435 AAGCTT A^AGCTT
NcoI 108 CCATGG C^CATGG
PshAI 180 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1331 TCATGA T^CATGA
SacI 1667 GAGCTC GAGCT^C
SalI 187 GTCGAC G^TCGAC
SphI 2823 GCATGC GCATG^C
XmnI 2538 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 187 GTCGAC GT^[AC][GT]AC
2158 GTATAC
AflII 988 CTTAAG C^TTAAG
2944 CTTAAG
AseI 735 ATTAAT AT^TAAT
1254 ATTAAT
AsnI 735 ATTAAT AT^TAAT
1254 ATTAAT
AspHI 1667 GAGCTC G[AT]GC[AT]^C
1925 GTGCAC
AvaII 473 GGACC G^G[AT]CC
1245 GGTCC
BalI 436 TGGCCA TGG^CCA
2411 TGGCCA
BanII 119 GAGCCC G[GA]GC[CT]^C
1667 GAGCTC
BbsI 1686 GAAGAC GAAGAC(2/6)
3082 GAAGAC
BclI 999 TGATCA T^GATCA
1305 TGATCA
BfrI 988 CTTAAG C^TTAAG
2944 CTTAAG
BpuAI 1686 GAAGAC GAAGAC(2/6)
3082 GAAGAC
BsaI 727 GAGACC GGTCTC(1/5)
1723 GAGACC
BsiHKAI 1667 GAGCTC G[AT]GC[AT]^C
1925 GTGCAC
BsmI 2482 GAATGC GAATGC(1/-1)
2584 GCATTC
Bsp1407I 663 TGTACA T^GTACA
1634 TGTACA
BsrGI 663 TGTACA T^GTACA
1634 TGTACA
BstAPI 25 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2584 GCATTCCTTGC
BstBI 779 TTCGAA TT^CGAA
1708 TTCGAA
BstF5I 166 CATCC GGATG(2/0)
2343 GGATG
BstXI 2018 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2196 CCAAGCTAATGG
BstYI 1566 GGATCT [GA]^GATC[CT]
2040 AGATCC
EaeI 436 TGGCCA [CT]^GGCC[GA]
2411 TGGCCA
FbaI 999 TGATCA T^GATCA
1305 TGATCA
FokI 166 CATCC GGATG(9/13)
2343 GGATG
HpaI 1696 GTTAAC GTT^AAC
2902 GTTAAC
MflI 1566 GGATCT [GA]^GATC[CT]
2040 AGATCC
MluNI 436 TGGCCA TGG^CCA
2411 TGGCCA
MscI 436 TGGCCA TGG^CCA
2411 TGGCCA
MslI 166 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1195 CATTTTGATG
NspV 779 TTCGAA TT^CGAA
1708 TTCGAA
PshBI 735 ATTAAT AT^TAAT
1254 ATTAAT
PstI 2186 CTGCAG CTGCA^G
2726 CTGCAG
ScaI 144 AGTACT AGT^ACT
2404 AGTACT
SfuI 779 TTCGAA TT^CGAA
1708 TTCGAA
SspBI 663 TGTACA T^GTACA
1634 TGTACA
Tsp45I 2788 GTGAC ^GT[GC]AC
2992 GTGAC
XhoII 1566 GGATCT [GA]^GATC[CT]
2040 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I