Restriction map for
pF1KA1997
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# NO CUT ENZYMES ( 53 enzymes) #
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AatII Acc65I AccII AcyI AgeI
ApaI AscI Asp718I AviII BbeI
BbrPI BglI BsaHI BsaI BsiWI
BsrFI BssHII BstBI BstUI Cfr10I
CpoI FseI FspI Hin1I KasI
KpnI KspI MluI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PinAI PmaCI PmeI PmlI PpuMI
PshAI RsrII SacII SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccIII 12786 TCCGGA T^CCGGA
AclI 11132 AACGTT AA^CGTT
AhdI 7696 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 7696 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1076 CTCGAG C^[CT]CG[GA]G
AvrII 3658 CCTAGG C^CTAGG
BbvCI 5572 CCTCAGC CCTCAGC(-5/-2)
BlnI 3658 CCTAGG C^CTAGG
BseAI 12786 TCCGGA T^CCGGA
BsoBI 1076 CTCGAG C^[CT]CG[GA]G
BspDI 5155 ATCGAT AT^CGAT
BspEI 12786 TCCGGA T^CCGGA
BsrBI 8018 GAGCGG CCGCTC(-3/-3)
BstEII 12840 GGTTACC G^GT[ACGT]ACC
BstPI 12840 GGTTACC G^GT[ACGT]ACC
ClaI 5155 ATCGAT AT^CGAT
DraIII 3761 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4153 GACAATAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4068 CGGCCG C^GGCCG
Eam1105I 7696 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4068 CGGCCG C^GGCCG
Eco52I 4068 CGGCCG C^GGCCG
EcoO65I 12840 GGTTACC G^GT[ACGT]ACC
FauI 11312 CCCGC CCCGC(4/6)
MroI 12786 TCCGGA T^CCGGA
NheI 10296 GCTAGC G^CTAGC
PacI 9523 TTAATTAA TTAAT^TAA
PaeR7I 1076 CTCGAG C^TCGAG
Psp1406I 11132 AACGTT AA^CGTT
PvuI 12195 CGATCG CGAT^CG
SalI 12617 GTCGAC G^TCGAC
SexAI 11252 ACCAGGT A^CC[AT]GGT
SpeI 3667 ACTAGT A^CTAGT
StuI 9768 AGGCCT AGG^CCT
XhoI 1076 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AflII 6727 CTTAAG C^TTAAG
8251 CTTAAG
Alw44I 6956 GTGCAC G^TGCAC
10403 GTGCAC
AocI 150 CCTCAGG CC^T[ACGT]AGG
11644 CCTCAGG
Aor51HI 920 AGCGCT AGC^GCT
2147 AGCGCT
ApaLI 6956 GTGCAC G^TGCAC
10403 GTGCAC
AspI 8678 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
9687 GACCAAGTC
BalI 3210 TGGCCA TGG^CCA
3431 TGGCCA
BamHI 4746 GGATCC G^GATCC
7540 GGATCC
BanI 1385 GGTGCC G^G[CT][GA]CC
11725 GGTGCC
BcgI 3372 CGAGAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
10719 GCATTTTGTTCG
BfrI 6727 CTTAAG C^TTAAG
8251 CTTAAG
BsaAI 3791 TACGTG [CT]AC^GT[GA]
10829 TACGTG
BsiEI 4068 CGGCCG CG[GA][CT]^CG
12195 CGATCG
BsmBI 3643 CGTCTC CGTCTC(1/5)
10020 GAGACG
BssSI 497 CACGAG CACGAG(-5/-1)
6647 CACGAG
Bst1107I 4950 GTATAC GTA^TAC
7033 GTATAC
BstZ17I 4950 GTATAC GTA^TAC
7033 GTATAC
Bsu36I 150 CCTCAGG CC^T[ACGT]AGG
11644 CCTCAGG
DraII 2365 AGGCCCC [GA]G^G[ACGT]CC[CT]
5203 GGGGCCT
Eco47III 920 AGCGCT AGC^GCT
2147 AGCGCT
Eco81I 150 CCTCAGG CC^T[ACGT]AGG
11644 CCTCAGG
EcoO109I 2365 AGGCCCC [GA]G^G[ACGT]CC[CT]
5203 GGGGCCT
HpaI 6268 GTTAAC GTT^AAC
10168 GTTAAC
MfeI 611 CAATTG C^AATTG
3716 CAATTG
MluNI 3210 TGGCCA TGG^CCA
3431 TGGCCA
MscI 3210 TGGCCA TGG^CCA
3431 TGGCCA
MunI 611 CAATTG C^AATTG
3716 CAATTG
PflFI 8678 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
9687 GACCAAGTC
SwaI 3746 ATTTAAAT ATTT^AAAT
4022 ATTTAAAT
Tth111I 8678 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
9687 GACCAAGTC
XcmI 3177 CCAACTAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7500 CCAATGGGTTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbsI BbvI BciVI BclI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoNI EcoRI EcoRII
EcoRV EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PshBI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII XmnI