Restriction map for
pF1KA1816
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp700I AspEI AspI BamHI
BbrPI BbvCI BcgI BciVI BfrI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BsrDI BssHII BssSI Bst1107I BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoRV EcoT22I
FseI HindIII HpaI KspI MamI
MfeI MluI MroI MunI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SmlI
SnaBI Sse8387I SwaI Tth111I XhoI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 281 GGTACC G^GTACC
AccII 30 CGCG CG^CG
Asp718I 281 GGTACC G^GTACC
AviII 2538 TGCGCA TGC^GCA
AvrII 2806 CCTAGG C^CTAGG
BglII 723 AGATCT A^GATCT
BlnI 2806 CCTAGG C^CTAGG
BsiEI 194 CGGCCG CG[GA][CT]^CG
BsmI 3243 GAATGC GAATGC(1/-1)
Bsp1407I 2597 TGTACA T^GTACA
BsrGI 2597 TGTACA T^GTACA
BstBI 575 TTCGAA TT^CGAA
BstEII 3391 GGTAACC G^GT[ACGT]ACC
BstPI 3391 GGTAACC G^GT[ACGT]ACC
BstUI 30 CGCG CG^CG
EagI 194 CGGCCG C^GGCCG
EclXI 194 CGGCCG C^GGCCG
Eco52I 194 CGGCCG C^GGCCG
EcoO65I 3391 GGTAACC G^GT[ACGT]ACC
FspI 2538 TGCGCA TGC^GCA
HgaI 1351 GCGTC GACGC(5/10)
KpnI 281 GGTACC GGTAC^C
MvnI 30 CGCG CG^CG
NaeI 379 GCCGGC GCC^GGC
NdeI 1568 CATATG CA^TATG
NgoMIV 379 GCCGGC G^CCGGC
NspV 575 TTCGAA TT^CGAA
PflMI 511 CCACTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1819 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 500 GAGCTC GAGCT^C
SapI 1071 GAAGAGC GCTCTTC(1/4)
ScaI 115 AGTACT AGT^ACT
SfuI 575 TTCGAA TT^CGAA
SmaI 3087 CCCGGG CCC^GGG
SspBI 2597 TGTACA T^GTACA
SspI 1771 AATATT AAT^ATT
Van91I 511 CCACTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 717 TCTAGA T^CTAGA
XcmI 3352 CCAGGGGACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3087 CCCGGG C^CCGGG
XmaI 3087 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 376 GGCGCC G[GA]^CG[CT]C
2938 GGCGCC
AlwI 89 GGATC GGATC(4/5)
1114 GGATC
ApaI 174 GGGCCC GGGCC^C
2228 GGGCCC
AseI 904 ATTAAT AT^TAAT
933 ATTAAT
AsnI 904 ATTAAT AT^TAAT
933 ATTAAT
AvaI 402 CCCGAG C^[CT]CG[GA]G
3087 CCCGGG
BbeI 376 GGCGCC GGCGC^C
2938 GGCGCC
BglI 176 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1960 GCCCCCAGGGC
BsaHI 376 GGCGCC G[GA]^CG[CT]C
2938 GGCGCC
BseRI 647 CTCCTC GAGGAG(10/8)
2880 GAGGAG
BsoBI 402 CCCGAG C^[CT]CG[GA]G
3087 CCCGGG
BspHI 2003 TCATGA T^CATGA
2201 TCATGA
BspLU11I 1101 ACATGT A^CATGT
1382 ACATGT
BsrBI 223 GAGCGG CCGCTC(-3/-3)
3238 GAGCGG
BstXI 525 CCAGCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2508 CCAGAACCCTGG
BstYI 723 AGATCT [GA]^GATC[CT]
1114 GGATCT
EcoNI 2801 CCTTGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3135 CCTGAGCCAGG
EcoRI 572 GAATTC G^AATTC
1018 GAATTC
Hin1I 376 GGCGCC G[GA]^CG[CT]C
2938 GGCGCC
HincII 265 GTCAAC GT[CT]^[GA]AC
766 GTCAAC
HindII 265 GTCAAC GT[CT]^[GA]AC
766 GTCAAC
KasI 376 GGCGCC G^GCGCC
2938 GGCGCC
MflI 723 AGATCT [GA]^GATC[CT]
1114 GGATCT
NarI 376 GGCGCC GG^CGCC
2938 GGCGCC
PshBI 904 ATTAAT AT^TAAT
933 ATTAAT
RcaI 2003 TCATGA T^CATGA
2201 TCATGA
SfoI 376 GGCGCC GGC^GCC
2938 GGCGCC
SpeI 1174 ACTAGT A^CTAGT
2908 ACTAGT
StuI 1129 AGGCCT AGG^CCT
2798 AGGCCT
XhoII 723 AGATCT [GA]^GATC[CT]
1114 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI AocI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I