Restriction map for
pF1KA1680
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspHI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 301 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 338 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 861 TGGCCA TGG^CCA
BamHI 1226 GGATCC G^GATCC
BglII 385 AGATCT A^GATCT
BsaI 33 GGTCTC GGTCTC(1/5)
BsaWI 1011 ACCGGA [AT]^CCGG[AT]
BsmBI 1632 CGTCTC CGTCTC(1/5)
Bsp1407I 2624 TGTACA T^GTACA
BsrGI 2624 TGTACA T^GTACA
BstAPI 1309 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 861 TGGCCA [CT]^GGCC[GA]
EcoNI 1816 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 622 GAATTC G^AATTC
EcoT14I 1171 CCAAGG C^C[AT][AT]GG
EcoT22I 815 ATGCAT ATGCA^T
MluNI 861 TGGCCA TGG^CCA
MscI 861 TGGCCA TGG^CCA
MslI 1297 CATGGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 815 ATGCAT ATGCA^T
PshAI 1939 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2047 CAGCTG CAG^CTG
SacI 2481 GAGCTC GAGCT^C
SexAI 282 ACCTGGT A^CC[AT]GGT
SspBI 2624 TGTACA T^GTACA
SspI 48 AATATT AAT^ATT
StyI 1171 CCAAGG C^C[AT][AT]GG
XcmI 1801 CCATCTGCGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 338 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 1215 ACATGT A^C[GA][CT]GT
2621 ACATGT
BanII 2481 GAGCTC G[GA]GC[CT]^C
2512 GGGCTC
BclI 1269 TGATCA T^GATCA
1755 TGATCA
BcnI 38 CCCGG CC^[GC]GG
1608 CCGGG
BseRI 177 GAGGAG GAGGAG(10/8)
1371 GAGGAG
BsgI 1881 CTGCAC GTGCAG(16/14)
1934 GTGCAG
BspLU11I 1215 ACATGT A^CATGT
2621 ACATGT
BstXI 599 CCATTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2688 CCAGCAGGATGG
Cac8I 1951 GCAAGC GC[ACGT]^[ACGT]GC
2687 GCCAGC
FbaI 1269 TGATCA T^GATCA
1755 TGATCA
HincII 525 GTCAAC GT[CT]^[GA]AC
2348 GTCAAC
HindII 525 GTCAAC GT[CT]^[GA]AC
2348 GTCAAC
MspA1I 2047 CAGCTG C[AC]G^C[GT]G
2312 CAGCGG
NciI 38 CCCGG CC^[GC]GG
1608 CCGGG
NlaIV 1226 GGATCC GG[ACGT]^[ACGT]CC
1665 GGAACC
NspI 1215 ACATGT [GA]CATG^[CT]
2621 ACATGT
PstI 1400 CTGCAG CTGCA^G
2044 CTGCAG
SapI 1990 GAAGAGC GCTCTTC(1/4)
2086 GAAGAGC
SmlI 150 CTCAAG C^T[CT][GA]AG
2038 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII