Restriction map for
pF1KA1611
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoT14I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MunI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
TfiI XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 627 GTCTAC GT^[AC][GT]AC
AlwI 89 GGATC GGATC(4/5)
AspI 143 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 75 GGACC G^G[AT]CC
BalI 38 TGGCCA TGG^CCA
BanII 193 GAGCCC G[GA]GC[CT]^C
BmyI 193 GAGCCC G[AGT]GC[ACT]^C
BseRI 58 GAGGAG GAGGAG(10/8)
BsmAI 509 GAGAC GTCTC(1/5)
Bsp1286I 193 GAGCCC G[AGT]GC[ACT]^C
BstBI 807 TTCGAA TT^CGAA
BstYI 89 GGATCT [GA]^GATC[CT]
Cac8I 1124 GCAAGC GC[ACGT]^[ACGT]GC
DraIII 340 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 38 TGGCCA [CT]^GGCC[GA]
EcoNI 6 CCTTACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 271 GATATC GAT^ATC
FauI 243 GCGGG CCCGC(4/6)
HincII 21 GTTGAC GT[CT]^[GA]AC
HindII 21 GTTGAC GT[CT]^[GA]AC
HinfI 2091 GACTC G^A[ACGT]TC
MaeII 104 ACGT A^CGT
MflI 89 GGATCT [GA]^GATC[CT]
MluNI 38 TGGCCA TGG^CCA
MscI 38 TGGCCA TGG^CCA
NdeI 2335 CATATG CA^TATG
NspV 807 TTCGAA TT^CGAA
PflFI 143 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 339 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2091 GACTC GAGTC(4/5)
PstI 574 CTGCAG CTGCA^G
SfcI 574 CTGCAG C^T[GA][CT]AG
SfuI 807 TTCGAA TT^CGAA
SspI 163 AATATT AAT^ATT
Tth111I 143 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 339 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 89 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 242 CGCG CG^CG
499 CGCG
BbvI 576 GCAGC GCAGC(8/12)
1457 GCAGC
BsaJI 196 CCCTGG C^C[ACGT][ACGT]GG
1317 CCTCGG
BsaWI 2098 ACCGGA [AT]^CCGG[AT]
2434 ACCGGA
BsiYI 6 CCTTACTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
339 CCACACAGTGG
BslI 6 CCTTACTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
339 CCACACAGTGG
BsmFI 101 GGGAC GGGAC(10/14)
141 GGGAC
Bsp1407I 286 TGTACA T^GTACA
2287 TGTACA
BsrGI 286 TGTACA T^GTACA
2287 TGTACA
BstUI 242 CGCG CG^CG
499 CGCG
HapII 2099 CCGG C^CGG
2435 CCGG
HindIII 523 AAGCTT A^AGCTT
549 AAGCTT
HpaII 2099 CCGG C^CGG
2435 CCGG
MseI 713 TTAA T^TAA
1081 TTAA
MspI 2099 CCGG C^CGG
2435 CCGG
MvnI 242 CGCG CG^CG
499 CGCG
SfaNI 2117 GATGC GCATC(5/9)
1326 GCATC
SspBI 286 TGTACA T^GTACA
2287 TGTACA
TaqI 808 TCGA T^CGA
1497 TCGA
Tru9I 713 TTAA T^TAA
1081 TTAA
TseI 576 GCAGC G^C[AT]GC
1457 GCAGC
Tsp45I 265 GTCAC ^GT[GC]AC
651 GTCAC
TthHB8I 808 TCGA T^CGA
1497 TCGA
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I BbsI BfaI BpmI BpuAI
BsmI BspHI BsrDI BsrI BssKI
BstAPI BstF5I BstNI Cfr13I DdeI
DpnII EarI EcoRI EcoRII EcoT22I
Fnu4HI FokI HaeIII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV NsiI RcaI RsaI
SapI Sau3AI Sau96I ScrFI Tsp509I
TspRI XmnI