Restriction map for
pF1KA1497
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcnI BfrI BglI BglII
BlnI BpmI BsaAI BsaI BsaWI
BseAI BseRI BsgI BsiWI BsmBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I CfoI Cfr10I ClaI
CpoI DraI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoT22I FauI FseI FspI HaeII
HapII HhaI HinP1I HpaII KasI
KspI MflI MluI MluNI MroI
MscI MspA1I MspI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1650 GACGTC GACGT^C
AccII 252 CGCG CG^CG
AcyI 1650 GACGTC G[GA]^CG[CT]C
AlwI 1134 GATCC GGATC(4/5)
AlwNI 1358 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1116 CCTCAGG CC^T[ACGT]AGG
AspHI 895 GAGCTC G[AT]GC[AT]^C
AvaI 1526 CTCGGG C^[CT]CG[GA]G
BbvCI 423 CCTCAGC CCTCAGC(-5/-2)
BciVI 1100 GTATCC GTATCC(6/5)
BclI 1872 TGATCA T^GATCA
BlpI 1351 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1351 GCTGAGC GC^T[ACGT]AGC
BsaBI 1095 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1650 GACGTC G[GA]^CG[CT]C
BsiEI 909 CGACCG CG[GA][CT]^CG
BsiHKAI 895 GAGCTC G[AT]GC[AT]^C
BsmAI 1784 GTCTC GTCTC(1/5)
BsmI 1047 GAATGC GAATGC(1/-1)
BsoBI 1526 CTCGGG C^[CT]CG[GA]G
BstUI 252 CGCG CG^CG
Bsu36I 1116 CCTCAGG CC^T[ACGT]AGG
CelII 1351 GCTGAGC GC^T[ACGT]AGC
DraIII 1222 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1812 GACTCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1346 CCATGG C^C[GA][CT]GG
EarI 2016 CTCTTC CTCTTC(1/4)
Eco81I 1116 CCTCAGG CC^T[ACGT]AGG
EcoRI 614 GAATTC G^AATTC
FbaI 1872 TGATCA T^GATCA
HaeIII 46 GGCC GG^CC
HgaI 1936 GCGTC GACGC(5/10)
Hin1I 1650 GACGTC G[GA]^CG[CT]C
HpaI 1546 GTTAAC GTT^AAC
Ksp632I 2016 CTCTTC CTCTTC(1/4)
MamI 1095 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 34 CAATTG C^AATTG
MunI 34 CAATTG C^AATTG
MvnI 252 CGCG CG^CG
NcoI 1346 CCATGG C^CATGG
NruI 251 TCGCGA TCG^CGA
PflMI 984 CCACCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 895 GAGCTC GAGCT^C
SexAI 1223 ACCAGGT A^CC[AT]GGT
SmlI 721 CTTGAG C^T[CT][GA]AG
SspI 1798 AATATT AAT^ATT
Van91I 984 CCACCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 1444 GGTACC G^GTACC
1567 GGTACC
AflIII 1492 ACATGT A^C[GA][CT]GT
2141 ACATGT
Asp718I 1444 GGTACC G^GTACC
1567 GGTACC
AvaII 1553 GGACC G^G[AT]CC
1719 GGTCC
BanII 895 GAGCTC G[GA]GC[CT]^C
1177 GAGCCC
BcgI 909 CGACCGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
242 GCAATAACATCG
BspHI 587 TCATGA T^CATGA
1646 TCATGA
BspLU11I 1492 ACATGT A^CATGT
2141 ACATGT
BsrDI 1941 CATTGC GCAATG(2/0)
173 GCAATG
DraII 1552 GGGACCC [GA]G^G[ACGT]CC[CT]
1718 GGGTCCC
EcoO109I 1552 GGGACCC [GA]G^G[ACGT]CC[CT]
1718 GGGTCCC
EcoRV 1275 GATATC GAT^ATC
1312 GATATC
HindIII 654 AAGCTT A^AGCTT
1020 AAGCTT
HphI 1227 GGTGA GGTGA(8/7)
2068 GGTGA
KpnI 1444 GGTACC GGTAC^C
1567 GGTACC
NspI 1492 ACATGT [GA]CATG^[CT]
2141 ACATGT
PpuMI 1552 GGGACCC [GA]G^G[AT]CC[CT]
1718 GGGTCCC
RcaI 587 TCATGA T^CATGA
1646 TCATGA
SalI 907 GTCGAC G^TCGAC
2119 GTCGAC
SfaNI 1026 GATGC GCATC(5/9)
1752 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI BanI BbsI BbvI BfaI
BmyI BpuAI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HincII
HindII HinfI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I