Restriction map for
pF1KA1401
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII AclI AcyI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BciVI BclI BglII
BlnI BsaAI BsaBI BsaHI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspMI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SbfI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 2236 GGTACC G^GTACC
AccII 166 CGCG CG^CG
AccIII 1526 TCCGGA T^CCGGA
AflII 1516 CTTAAG C^TTAAG
AflIII 2144 ACATGT A^C[GA][CT]GT
AgeI 505 ACCGGT A^CCGGT
AocI 1546 CCTAAGG CC^T[ACGT]AGG
Aor51HI 638 AGCGCT AGC^GCT
Asp700I 1552 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2236 GGTACC G^GTACC
AspHI 1837 GAGCTC G[AT]GC[AT]^C
BalI 2244 TGGCCA TGG^CCA
BamHI 1542 GGATCC G^GATCC
BanII 1837 GAGCTC G[GA]GC[CT]^C
BbsI 2317 GTCTTC GAAGAC(2/6)
BcgI 60 GCATCGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1516 CTTAAG C^TTAAG
BglI 1945 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1125 GCTGAGC GC^T[ACGT]AGC
BpmI 2208 CTGGAG CTGGAG(16/14)
Bpu1102I 1125 GCTGAGC GC^T[ACGT]AGC
BpuAI 2317 GTCTTC GAAGAC(2/6)
BsaI 917 GAGACC GGTCTC(1/5)
BseAI 1526 TCCGGA T^CCGGA
BsiHKAI 1837 GAGCTC G[AT]GC[AT]^C
BsmBI 1698 CGTCTC CGTCTC(1/5)
BsmI 2021 GAATGC GAATGC(1/-1)
BspEI 1526 TCCGGA T^CCGGA
BspHI 1758 TCATGA T^CATGA
BspLU11I 2144 ACATGT A^CATGT
BsrBI 22 CCGCTC CCGCTC(-3/-3)
BsrFI 505 ACCGGT [GA]^CCGG[CT]
BstUI 166 CGCG CG^CG
Bsu36I 1546 CCTAAGG CC^T[ACGT]AGG
CelII 1125 GCTGAGC GC^T[ACGT]AGC
Cfr10I 505 ACCGGT [GA]^CCGG[CT]
DraIII 430 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2068 GACAGAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2244 TGGCCA [CT]^GGCC[GA]
Eco47III 638 AGCGCT AGC^GCT
Eco81I 1546 CCTAAGG CC^T[ACGT]AGG
EcoRI 1490 GAATTC G^AATTC
HincII 151 GTCGAC GT[CT]^[GA]AC
HindII 151 GTCGAC GT[CT]^[GA]AC
KpnI 2236 GGTACC GGTAC^C
MluNI 2244 TGGCCA TGG^CCA
MroI 1526 TCCGGA T^CCGGA
MscI 2244 TGGCCA TGG^CCA
MvnI 166 CGCG CG^CG
NdeI 2342 CATATG CA^TATG
NspI 2144 ACATGT [GA]CATG^[CT]
PinAI 505 ACCGGT A^CCGGT
PstI 1898 CTGCAG CTGCA^G
RcaI 1758 TCATGA T^CATGA
SacI 1837 GAGCTC GAGCT^C
SalI 151 GTCGAC G^TCGAC
SapI 1834 GAAGAGC GCTCTTC(1/4)
ScaI 2292 AGTACT AGT^ACT
SgrAI 504 CACCGGTG C[GA]^CCGG[CT]G
SspI 1578 AATATT AAT^ATT
StuI 1512 AGGCCT AGG^CCT
XmnI 1552 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 151 GTCGAC GT^[AC][GT]AC
1049 GTAGAC
AvaII 960 GGACC G^G[AT]CC
1176 GGTCC
BcnI 16 CCCGG CC^[GC]GG
913 CCCGG
BsaWI 505 ACCGGT [AT]^CCGG[AT]
1526 TCCGGA
BstXI 1230 CCAAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2356 CCAGTACCCTGG
DraI 930 TTTAAA TTT^AAA
1621 TTTAAA
DsaI 1537 CCATGG C^C[GA][CT]GG
2241 CCATGG
HaeII 638 AGCGCT [GA]GCGC^[CT]
1862 GGCGCT
MaeII 431 ACGT A^CGT
2139 ACGT
NciI 16 CCCGG CC^[GC]GG
913 CCCGG
NcoI 1537 CCATGG C^CATGG
2241 CCATGG
PpuMI 959 AGGACCC [GA]G^G[AT]CC[CT]
1175 AGGTCCC
PvuII 220 CAGCTG CAG^CTG
301 CAGCTG
Tsp45I 1672 GTCAC ^GT[GC]AC
1799 GTGAC
XcmI 484 CCACTAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
567 CCAGGGGATTTCTGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BanI BbvCI BbvI
BfaI BmyI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII