Restriction map for
pF1KA1261
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbeI BbvCI BcgI
BfrI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrFI BssHII Bst1107I BstBI BstZ17I
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcsI 73 AAATTT [GA]^AATT[CT]
AcyI 1399 GACGCC G[GA]^CG[CT]C
Alw44I 284 GTGCAC G^TGCAC
AocI 1077 CCTAAGG CC^T[ACGT]AGG
ApaLI 284 GTGCAC G^TGCAC
ApoI 73 AAATTT [GA]^AATT[CT]
Asp700I 109 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 422 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 797 CTCGAG C^[CT]CG[GA]G
BalI 1748 TGGCCA TGG^CCA
BamHI 783 GGATCC G^GATCC
BbrPI 1501 CACGTG CAC^GTG
BbsI 177 GAAGAC GAAGAC(2/6)
BciVI 619 GTATCC GTATCC(6/5)
BclI 579 TGATCA T^GATCA
BglI 1547 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1985 AGATCT A^GATCT
BlpI 2235 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2235 GCTTAGC GC^T[ACGT]AGC
BpuAI 177 GAAGAC GAAGAC(2/6)
BsaAI 1501 CACGTG [CT]AC^GT[GA]
BsaHI 1399 GACGCC G[GA]^CG[CT]C
BsaI 427 GGTCTC GGTCTC(1/5)
BsmBI 957 CGTCTC CGTCTC(1/5)
BsoBI 797 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1505 TGTACA T^GTACA
BspHI 2097 TCATGA T^CATGA
BsrBI 1169 GAGCGG CCGCTC(-3/-3)
BsrDI 2045 GCAATG GCAATG(2/0)
BsrGI 1505 TGTACA T^GTACA
BssSI 1494 CACGAG CACGAG(-5/-1)
BstAPI 1213 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1851 CCACACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1077 CCTAAGG CC^T[ACGT]AGG
CelII 2235 GCTTAGC GC^T[ACGT]AGC
DraI 70 TTTAAA TTT^AAA
DrdI 1918 GACAACACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1748 TGGCCA [CT]^GGCC[GA]
Eco81I 1077 CCTAAGG CC^T[ACGT]AGG
FbaI 579 TGATCA T^GATCA
Hin1I 1399 GACGCC G[GA]^CG[CT]C
MluNI 1748 TGGCCA TGG^CCA
MscI 1748 TGGCCA TGG^CCA
NcoI 362 CCATGG C^CATGG
NruI 6 TCGCGA TCG^CGA
PaeR7I 797 CTCGAG C^TCGAG
PflFI 422 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1501 CACGTG CAC^GTG
PmlI 1501 CACGTG CAC^GTG
RcaI 2097 TCATGA T^CATGA
SacI 609 GAGCTC GAGCT^C
SapI 611 GCTCTTC GCTCTTC(1/4)
SphI 1428 GCATGC GCATG^C
SspBI 1505 TGTACA T^GTACA
StuI 1030 AGGCCT AGG^CCT
Tth111I 422 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 797 CTCGAG C^TCGAG
XmnI 109 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 1500 ACACGT A^C[GA][CT]GT
1502 ACGTGT
AlwNI 1214 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1929 CAGGTCCTG
BanI 45 GGCACC G^G[CT][GA]CC
1891 GGCACC
BfaI 526 CTAG C^TAG
835 CTAG
BseRI 675 CTCCTC GAGGAG(10/8)
909 CTCCTC
BsgI 1816 CTGCAC GTGCAG(16/14)
331 GTGCAG
BspMI 1940 ACCTGC ACCTGC(4/8)
354 GCAGGT
BstEII 357 GGTGACC G^GT[ACGT]ACC
1476 GGTGACC
BstPI 357 GGTGACC G^GT[ACGT]ACC
1476 GGTGACC
EarI 612 CTCTTC CTCTTC(1/4)
109 GAAGAG
EcoNI 1829 CCTTGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1941 CCTGCGCGAGG
EcoO65I 357 GGTGACC G^GT[ACGT]ACC
1476 GGTGACC
FauI 27 CCCGC CCCGC(4/6)
1732 CCCGC
HgaI 1399 GACGC GACGC(5/10)
1526 GCGTC
HincII 766 GTTGAC GT[CT]^[GA]AC
1057 GTTGAC
HindII 766 GTTGAC GT[CT]^[GA]AC
1057 GTTGAC
Ksp632I 612 CTCTTC CTCTTC(1/4)
109 GAAGAG
MaeI 526 CTAG C^TAG
835 CTAG
MaeII 770 ACGT A^CGT
1502 ACGT
PflMI 1824 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1895 CCAAGCTCTGG
ScaI 901 AGTACT AGT^ACT
1663 AGTACT
TaqI 798 TCGA T^CGA
1580 TCGA
TthHB8I 798 TCGA T^CGA
1580 TCGA
Van91I 1824 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1895 CCAAGCTCTGG
XcmI 1673 CCATTTGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2008 CCAACTGGAGAGTGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII