Restriction map for
pF1KA1255
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AscI AseI AsnI Asp718I AvrII
BamHI BbvCI BfrI BlnI BsiWI
BsmBI BspDI BspHI BsrBI BsrDI
BssHII Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EcoO65I
FseI HindIII HpaI KpnI MfeI
MluI MunI NcoI NheI NotI
NruI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I RcaI RsrII
SalI SbfI ScaI SfiI SmaI
SmlI SnaBI SpeI Sse8387I SspI
StuI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 3515 GACGTC GACGT^C
AhdI 1975 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 77 GGGCCC GGGCC^C
Asp700I 1386 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1975 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 240 TGCGCA TGC^GCA
BbrPI 2548 CACGTG CAC^GTG
BcgI 467 CGATGACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3228 GTATCC GTATCC(6/5)
BlpI 2716 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2716 GCTGAGC GC^T[ACGT]AGC
BsaAI 2548 CACGTG [CT]AC^GT[GA]
BsaBI 448 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1889 GAGACC GGTCTC(1/5)
Bsp1407I 1121 TGTACA T^GTACA
BsrGI 1121 TGTACA T^GTACA
BssSI 1321 CACGAG CACGAG(-5/-1)
BstBI 1393 TTCGAA TT^CGAA
CelII 2716 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1975 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2817 GAATTC G^AATTC
EcoRV 2605 GATATC GAT^ATC
EcoT22I 3216 ATGCAT ATGCA^T
FspI 240 TGCGCA TGC^GCA
MamI 448 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2145 CATATG CA^TATG
NsiI 3216 ATGCAT ATGCA^T
NspV 1393 TTCGAA TT^CGAA
PmaCI 2548 CACGTG CAC^GTG
PmlI 2548 CACGTG CAC^GTG
PvuI 1934 CGATCG CGAT^CG
SacI 1651 GAGCTC GAGCT^C
SfuI 1393 TTCGAA TT^CGAA
SgrAI 54 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 1121 TGTACA T^GTACA
XcmI 425 CCACTAAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1386 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 37 enzymes) #
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AccIII 1791 TCCGGA T^CCGGA
1863 TCCGGA
AcsI 2742 GAATTT [GA]^AATT[CT]
2817 GAATTC
AflIII 1239 ACATGT A^C[GA][CT]GT
2165 ACATGT
Alw44I 3108 GTGCAC G^TGCAC
3462 GTGCAC
AlwI 485 GGATC GGATC(4/5)
882 GGATC
Aor51HI 221 AGCGCT AGC^GCT
742 AGCGCT
ApaLI 3108 GTGCAC G^TGCAC
3462 GTGCAC
ApoI 2742 GAATTT [GA]^AATT[CT]
2817 GAATTC
AspI 820 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1741 GACAGCGTC
BbeI 1666 GGCGCC GGCGC^C
2398 GGCGCC
BclI 764 TGATCA T^GATCA
2795 TGATCA
BseAI 1791 TCCGGA T^CCGGA
1863 TCCGGA
BspEI 1791 TCCGGA T^CCGGA
1863 TCCGGA
BspLU11I 1239 ACATGT A^CATGT
2165 ACATGT
EagI 21 CGGCCG C^GGCCG
3072 CGGCCG
EclXI 21 CGGCCG C^GGCCG
3072 CGGCCG
Eco47III 221 AGCGCT AGC^GCT
742 AGCGCT
Eco52I 21 CGGCCG C^GGCCG
3072 CGGCCG
EcoNI 714 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1182 CCTTCTGCAGG
EcoT14I 3422 CCAAGG C^C[AT][AT]GG
3542 CCAAGG
FbaI 764 TGATCA T^GATCA
2795 TGATCA
KasI 1666 GGCGCC G^GCGCC
2398 GGCGCC
KspI 10 CCGCGG CCGC^GG
72 CCGCGG
MroI 1791 TCCGGA T^CCGGA
1863 TCCGGA
NarI 1666 GGCGCC GG^CGCC
2398 GGCGCC
PflFI 820 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1741 GACAGCGTC
PflMI 1944 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2267 CCACATGCTGG
SacII 10 CCGCGG CCGC^GG
72 CCGCGG
SapI 1831 GCTCTTC GCTCTTC(1/4)
3049 GCTCTTC
SexAI 2286 ACCTGGT A^CC[AT]GGT
3374 ACCAGGT
SfoI 1666 GGCGCC GGC^GCC
2398 GGCGCC
SphI 1925 GCATGC GCATG^C
3218 GCATGC
StyI 3422 CCAAGG C^C[AT][AT]GG
3542 CCAAGG
TaqI 1394 TCGA T^CGA
3538 TCGA
Tth111I 820 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1741 GACAGCGTC
TthHB8I 1394 TCGA T^CGA
3538 TCGA
Van91I 1944 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2267 CCACATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII