Restriction map for
pF1KA0983
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspHI AvaI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BglII BlnI BsaAI BsaHI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I Cfr10I
CpoI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I EcoRI EcoT22I
FseI FspI HgaI Hin1I HpaI
KasI KpnI KspI MluI MluNI
MroI MscI MslI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 1951 GAATTT [GA]^AATT[CT]
AhdI 1177 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2026 CCTCAGG CC^T[ACGT]AGG
ApaI 1942 GGGCCC GGGCC^C
ApoI 1951 GAATTT [GA]^AATT[CT]
AspEI 1177 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1192 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 263 GGATCC G^GATCC
BanI 1028 GGCACC G^G[CT][GA]CC
BbsI 1437 GAAGAC GAAGAC(2/6)
BciVI 1212 GTATCC GTATCC(6/5)
BclI 1154 TGATCA T^GATCA
BpuAI 1437 GAAGAC GAAGAC(2/6)
BsaBI 370 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 723 TCCGGT [AT]^CCGG[AT]
BspDI 77 ATCGAT AT^CGAT
BssSI 349 CACGAG CACGAG(-5/-1)
BstEII 1312 GGTCACC G^GT[ACGT]ACC
BstPI 1312 GGTCACC G^GT[ACGT]ACC
Bsu36I 2026 CCTCAGG CC^T[ACGT]AGG
ClaI 77 ATCGAT AT^CGAT
DraI 2011 TTTAAA TTT^AAA
Eam1105I 1177 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2026 CCTCAGG CC^T[ACGT]AGG
EcoNI 417 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1312 GGTCACC G^GT[ACGT]ACC
EcoRV 514 GATATC GAT^ATC
FbaI 1154 TGATCA T^GATCA
HincII 1863 GTTGAC GT[CT]^[GA]AC
HindII 1863 GTTGAC GT[CT]^[GA]AC
HindIII 298 AAGCTT A^AGCTT
MamI 370 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1571 CAATTG C^AATTG
MunI 1571 CAATTG C^AATTG
PflFI 1192 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
StuI 839 AGGCCT AGG^CCT
SwaI 2010 ATTTAAAT ATTT^AAAT
Tth111I 1192 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1607 CCAAAGACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaII 138 GGACC G^G[AT]CC
1990 GGTCC
BanII 53 GAGCCC G[GA]GC[CT]^C
1942 GGGCCC
BlpI 355 GCTAAGC GC^T[ACGT]AGC
1398 GCTGAGC
Bpu1102I 355 GCTAAGC GC^T[ACGT]AGC
1398 GCTGAGC
BstXI 434 CCAAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1406 CCAGCCACATGG
CelII 355 GCTAAGC GC^T[ACGT]AGC
1398 GCTGAGC
HapII 724 CCGG C^CGG
972 CCGG
HpaII 724 CCGG C^CGG
972 CCGG
MaeII 567 ACGT A^CGT
853 ACGT
MspI 724 CCGG C^CGG
972 CCGG
PpuMI 137 GGGACCC [GA]G^G[AT]CC[CT]
1989 GGGTCCT
SapI 216 GAAGAGC GCTCTTC(1/4)
1980 GCTCTTC
SmlI 646 CTCAAG C^T[CT][GA]AG
1303 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII