Restriction map for
pF1KA0767
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
AlwI AocI ApaI ApoI AscI
AseI AsnI AspEI AviII AvrII
BamHI BbeI BbrPI BcgI BclI
BfrI BlnI BsaBI BsiWI BspDI
BspHI BspLU11I Bst1107I BstXI BstZ17I
Bsu36I ClaI CpoI DraIII Eam1105I
EarI Eco81I EcoRI EcoRV EcoT22I
FbaI FspI HindIII KasI Ksp632I
MamI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SpeI
SphI Sse8387I SspI SwaI TfiI
XbaI XhoI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AatII 910 GACGTC GACGT^C
Acc65I 117 GGTACC G^GTACC
AccIII 293 TCCGGA T^CCGGA
AflIII 695 ACACGT A^C[GA][CT]GT
Alw44I 1654 GTGCAC G^TGCAC
Aor51HI 1211 AGCGCT AGC^GCT
ApaLI 1654 GTGCAC G^TGCAC
Asp718I 117 GGTACC G^GTACC
BalI 185 TGGCCA TGG^CCA
BbsI 871 GAAGAC GAAGAC(2/6)
BciVI 818 GGATAC GTATCC(6/5)
BglI 1033 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1346 AGATCT A^GATCT
BlpI 421 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 421 GCTCAGC GC^T[ACGT]AGC
BpuAI 871 GAAGAC GAAGAC(2/6)
BsaI 1675 GAGACC GGTCTC(1/5)
BseAI 293 TCCGGA T^CCGGA
BsgI 382 GTGCAG GTGCAG(16/14)
BsmBI 615 GAGACG CGTCTC(1/5)
Bsp1407I 719 TGTACA T^GTACA
BspEI 293 TCCGGA T^CCGGA
BspMI 596 GCAGGT ACCTGC(4/8)
BsrGI 719 TGTACA T^GTACA
BssHII 1146 GCGCGC G^CGCGC
BstBI 1489 TTCGAA TT^CGAA
BstEII 244 GGTCACC G^GT[ACGT]ACC
BstPI 244 GGTCACC G^GT[ACGT]ACC
BstYI 1346 AGATCT [GA]^GATC[CT]
CelII 421 GCTCAGC GC^T[ACGT]AGC
DraI 1126 TTTAAA TTT^AAA
DrdI 1245 GACGACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1280 CGGCCG C^GGCCG
EclXI 1280 CGGCCG C^GGCCG
Eco47III 1211 AGCGCT AGC^GCT
Eco52I 1280 CGGCCG C^GGCCG
EcoNI 1511 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 244 GGTCACC G^GT[ACGT]ACC
EcoT14I 1649 CCATGG C^C[AT][AT]GG
FseI 1281 GGCCGGCC GGCCGG^CC
HpaI 600 GTTAAC GTT^AAC
KpnI 117 GGTACC GGTAC^C
KspI 121 CCGCGG CCGC^GG
MflI 1346 AGATCT [GA]^GATC[CT]
MluNI 185 TGGCCA TGG^CCA
MroI 293 TCCGGA T^CCGGA
MscI 185 TGGCCA TGG^CCA
MslI 1657 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1282 GCCGGC GCC^GGC
NcoI 1649 CCATGG C^CATGG
NgoMIV 1282 GCCGGC G^CCGGC
NspV 1489 TTCGAA TT^CGAA
PflMI 806 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1303 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 898 CAGCTG CAG^CTG
SacI 289 GAGCTC GAGCT^C
SacII 121 CCGCGG CCGC^GG
SalI 1611 GTCGAC G^TCGAC
SfuI 1489 TTCGAA TT^CGAA
SmaI 134 CCCGGG CCC^GGG
SnaBI 1264 TACGTA TAC^GTA
SspBI 719 TGTACA T^GTACA
StuI 1667 AGGCCT AGG^CCT
StyI 1649 CCATGG C^C[AT][AT]GG
Van91I 806 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 447 CCAGGCCAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1346 AGATCT [GA]^GATC[CT]
XmaCI 134 CCCGGG C^CCGGG
XmaI 134 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 691 GTCTAC GT^[AC][GT]AC
1611 GTCGAC
AspI 879 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1248 GACCTCGTC
BanII 289 GAGCTC G[GA]GC[CT]^C
850 GAGCCC
BbvCI 1706 CCTCAGC CCTCAGC(-5/-2)
150 GCTGAGG
BcnI 134 CCCGG CC^[GC]GG
135 CCGGG
BsaAI 529 TACGTG [CT]AC^GT[GA]
1264 TACGTA
BsiEI 1280 CGGCCG CG[GA][CT]^CG
1308 CGGTCG
BsmI 91 GAATGC GAATGC(1/-1)
726 GAATGC
BsrDI 705 CATTGC GCAATG(2/0)
455 GCAATG
BsrI 680 ACTGG ACTGG(1/-1)
1689 CCAGT
BssSI 1342 CACGAG CACGAG(-5/-1)
1636 CTCGTG
BstAPI 375 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
384 GCAGGAGGTGC
EaeI 185 TGGCCA [CT]^GGCC[GA]
1280 CGGCCG
HincII 600 GTTAAC GT[CT]^[GA]AC
1611 GTCGAC
HindII 600 GTTAAC GT[CT]^[GA]AC
1611 GTCGAC
NciI 134 CCCGG CC^[GC]GG
135 CCGGG
PflFI 879 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1248 GACCTCGTC
PstI 496 CTGCAG CTGCA^G
1240 CTGCAG
SfcI 496 CTGCAG C^T[GA][CT]AG
1240 CTGCAG
SmlI 622 CTCAAG C^T[CT][GA]AG
1204 CTCAAG
TspRI 564 CACTG CA[GC]TG[ACGT][ACGT]^
823 CAGTG
Tth111I 879 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1248 GACCTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI Asp700I AspHI AvaI AvaII
BanI BbvI BfaI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI Tru9I
TseI Tsp45I Tsp509I TthHB8I XmnI