Restriction map for
pF1KB7020
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbrPI BfrI BglII BlnI
BlpI Bpu1102I BseAI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI Eam1105I EcoRI EcoRV
EcoT22I HindIII HpaI KpnI KspI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 1886 ACGTGT A^C[GA][CT]GT
Alw44I 2263 GTGCAC G^TGCAC
Aor51HI 2719 AGCGCT AGC^GCT
ApaLI 2263 GTGCAC G^TGCAC
AviII 533 TGCGCA TGC^GCA
BbeI 589 GGCGCC GGCGC^C
BcgI 2271 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2945 GTATCC GTATCC(6/5)
BclI 2067 TGATCA T^GATCA
BfaI 2453 CTAG C^TAG
BsaBI 2397 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2633 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1115 TGTACA T^GTACA
BspHI 2492 TCATGA T^CATGA
BsrBI 1429 GAGCGG CCGCTC(-3/-3)
BsrGI 1115 TGTACA T^GTACA
BssHII 615 GCGCGC G^CGCGC
BstAPI 1130 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1169 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2752 GACCGCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2719 AGCGCT AGC^GCT
EcoNI 2037 CCTAAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2067 TGATCA T^GATCA
FseI 2916 GGCCGGCC GGCCGG^CC
FspI 533 TGCGCA TGC^GCA
KasI 589 GGCGCC G^GCGCC
MaeI 2453 CTAG C^TAG
MamI 2397 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 589 GGCGCC GG^CGCC
RcaI 2492 TCATGA T^CATGA
SacI 322 GAGCTC GAGCT^C
SalI 1949 GTCGAC G^TCGAC
SapI 1476 GAAGAGC GCTCTTC(1/4)
ScaI 1466 AGTACT AGT^ACT
SfoI 589 GGCGCC GGC^GCC
SmaI 1085 CCCGGG CCC^GGG
SspBI 1115 TGTACA T^GTACA
StuI 2045 AGGCCT AGG^CCT
XcmI 172 CCAGAAAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1085 CCCGGG C^CCGGG
XmaI 1085 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 313 GTCTAC GT^[AC][GT]AC
1949 GTCGAC
AcsI 1927 GAATTT [GA]^AATT[CT]
2650 AAATTC
AcyI 589 GGCGCC G[GA]^CG[CT]C
1546 GACGCC
AocI 1743 CCTCAGG CC^T[ACGT]AGG
2640 CCTCAGG
ApaI 895 GGGCCC GGGCC^C
1646 GGGCCC
ApoI 1927 GAATTT [GA]^AATT[CT]
2650 AAATTC
AspHI 322 GAGCTC G[AT]GC[AT]^C
2263 GTGCAC
BalI 2 TGGCCA TGG^CCA
1985 TGGCCA
BbsI 2887 GAAGAC GAAGAC(2/6)
1705 GTCTTC
BbvCI 425 CCTCAGC CCTCAGC(-5/-2)
1157 CCTCAGC
BglI 36 GCCGCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2416 GCCCCAGAGGC
BpuAI 2887 GAAGAC GAAGAC(2/6)
1705 GTCTTC
BsaAI 316 TACGTG [CT]AC^GT[GA]
460 TACGTG
BsaHI 589 GGCGCC G[GA]^CG[CT]C
1546 GACGCC
BseRI 432 CTCCTC GAGGAG(10/8)
1906 GAGGAG
BsiEI 513 CGGCCG CG[GA][CT]^CG
1325 CGGCCG
BsiHKAI 322 GAGCTC G[AT]GC[AT]^C
2263 GTGCAC
BstEII 2100 GGTCACC G^GT[ACGT]ACC
2907 GGTCACC
BstPI 2100 GGTCACC G^GT[ACGT]ACC
2907 GGTCACC
BstYI 2396 AGATCC [GA]^GATC[CT]
2933 AGATCC
Bsu36I 1743 CCTCAGG CC^T[ACGT]AGG
2640 CCTCAGG
EagI 513 CGGCCG C^GGCCG
1325 CGGCCG
EclXI 513 CGGCCG C^GGCCG
1325 CGGCCG
Eco52I 513 CGGCCG C^GGCCG
1325 CGGCCG
Eco81I 1743 CCTCAGG CC^T[ACGT]AGG
2640 CCTCAGG
EcoO65I 2100 GGTCACC G^GT[ACGT]ACC
2907 GGTCACC
Hin1I 589 GGCGCC G[GA]^CG[CT]C
1546 GACGCC
MflI 2396 AGATCC [GA]^GATC[CT]
2933 AGATCC
MluNI 2 TGGCCA TGG^CCA
1985 TGGCCA
MscI 2 TGGCCA TGG^CCA
1985 TGGCCA
NcoI 1140 CCATGG C^CATGG
2579 CCATGG
PaeR7I 1069 CTCGAG C^TCGAG
2089 CTCGAG
PpuMI 1088 GGGACCT [GA]G^G[AT]CC[CT]
1844 AGGACCC
PstI 1789 CTGCAG CTGCA^G
2962 CTGCAG
TfiI 216 GAATC G^A[AT]TC
1768 GATTC
XhoI 1069 CTCGAG C^TCGAG
2089 CTCGAG
XhoII 2396 AGATCC [GA]^GATC[CT]
2933 AGATCC
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I