Restriction map for
pF1KB5705
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
AspI AvrII BamHI BbeI BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BseAI BsiWI BspDI
BspEI BspHI BspLU11I Bst1107I BstBI
BstEII BstPI BstYI BstZ17I ClaI
CpoI DraIII DrdI EcoO65I EcoRV
EcoT22I FauI FbaI FseI HindIII
HpaI KasI KspI MamI MflI
MluI MroI NarI NheI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsrII SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tth111I XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 192 GACGTC GACGT^C
Acc65I 83 GGTACC G^GTACC
AcsI 1453 GAATTC [GA]^AATT[CT]
AhdI 1777 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 78 GGATC GGATC(4/5)
AocI 1684 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1075 AGCGCT AGC^GCT
ApoI 1453 GAATTC [GA]^AATT[CT]
Asp700I 231 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 83 GGTACC G^GTACC
AspEI 1777 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 496 CTCGAG C^[CT]CG[GA]G
BbsI 1417 GTCTTC GAAGAC(2/6)
BbvCI 450 GCTGAGG CCTCAGC(-5/-2)
BcgI 2158 GCACTGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1078 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1078 GCTGAGC GC^T[ACGT]AGC
BpuAI 1417 GTCTTC GAAGAC(2/6)
BsaI 670 GAGACC GGTCTC(1/5)
BseRI 1195 CTCCTC GAGGAG(10/8)
BsmI 876 GAATGC GAATGC(1/-1)
BsoBI 496 CTCGAG C^[CT]CG[GA]G
BspMI 1268 ACCTGC ACCTGC(4/8)
BssHII 1174 GCGCGC G^CGCGC
BssSI 951 CACGAG CACGAG(-5/-1)
Bsu36I 1684 CCTGAGG CC^T[ACGT]AGG
CelII 1078 GCTGAGC GC^T[ACGT]AGC
DraI 2209 TTTAAA TTT^AAA
EagI 1491 CGGCCG C^GGCCG
Eam1105I 1777 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1966 CTCTTC CTCTTC(1/4)
EclXI 1491 CGGCCG C^GGCCG
Eco47III 1075 AGCGCT AGC^GCT
Eco52I 1491 CGGCCG C^GGCCG
Eco81I 1684 CCTGAGG CC^T[ACGT]AGG
EcoRI 1453 GAATTC G^AATTC
HincII 1774 GTCGAC GT[CT]^[GA]AC
HindII 1774 GTCGAC GT[CT]^[GA]AC
HinfI 2032 GACTC G^A[ACGT]TC
KpnI 83 GGTACC GGTAC^C
Ksp632I 1966 CTCTTC CTCTTC(1/4)
MaeII 193 ACGT A^CGT
MseI 2210 TTAA T^TAA
NaeI 185 GCCGGC GCC^GGC
NcoI 1983 CCATGG C^CATGG
NdeI 1154 CATATG CA^TATG
NgoMIV 185 GCCGGC G^CCGGC
NotI 1490 GCGGCCGC GC^GGCCGC
NspI 818 ACATGC [GA]CATG^[CT]
PaeR7I 496 CTCGAG C^TCGAG
PleI 2032 GACTC GAGTC(4/5)
PvuI 919 CGATCG CGAT^CG
SacI 1411 GAGCTC GAGCT^C
SalI 1774 GTCGAC G^TCGAC
Tru9I 2210 TTAA T^TAA
Tsp45I 1033 GTCAC ^GT[GC]AC
XcmI 1811 CCAATGGCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 496 CTCGAG C^TCGAG
XmnI 231 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1747 GTAGAC GT^[AC][GT]AC
1774 GTCGAC
AcyI 192 GACGTC G[GA]^CG[CT]C
214 GGCGTC
AspHI 925 GTGCTC G[AT]GC[AT]^C
1411 GAGCTC
AviII 809 TGCGCA TGC^GCA
1064 TGCGCA
BalI 977 TGGCCA TGG^CCA
2172 TGGCCA
BfaI 1006 CTAG C^TAG
1162 CTAG
BglI 1103 GCCCGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1721 GCCTCATGGGC
BsaHI 192 GACGTC G[GA]^CG[CT]C
214 GGCGTC
BsaWI 1122 TCCGGT [AT]^CCGG[AT]
1751 ACCGGA
BsiEI 919 CGATCG CG[GA][CT]^CG
1491 CGGCCG
BsiHKAI 925 GTGCTC G[AT]GC[AT]^C
1411 GAGCTC
BsmBI 216 CGTCTC CGTCTC(1/5)
391 GAGACG
BsmFI 1265 GGGAC GGGAC(10/14)
1308 GTCCC
BsrBI 826 GAGCGG CCGCTC(-3/-3)
862 GAGCGG
BsrDI 262 GCAATG GCAATG(2/0)
1994 GCAATG
BsrFI 185 GCCGGC [GA]^CCGG[CT]
1099 ACCGGC
Cfr10I 185 GCCGGC [GA]^CCGG[CT]
1099 ACCGGC
EcoNI 252 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
993 CCTGCGCCAGG
FspI 809 TGCGCA TGC^GCA
1064 TGCGCA
Hin1I 192 GACGTC G[GA]^CG[CT]C
214 GGCGTC
MaeI 1006 CTAG C^TAG
1162 CTAG
MaeIII 1033 GTCAC ^GT[ACGT]AC
1912 GTAAC
MfeI 536 CAATTG C^AATTG
1400 CAATTG
MluNI 977 TGGCCA TGG^CCA
2172 TGGCCA
MscI 977 TGGCCA TGG^CCA
2172 TGGCCA
MunI 536 CAATTG C^AATTG
1400 CAATTG
SmlI 496 CTCGAG C^T[CT][GA]AG
898 CTTGAG
StuI 383 AGGCCT AGG^CCT
851 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TaqI TseI Tsp509I TspRI TthHB8I
Van91I