Restriction map for
pF1KB5514
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoT22I
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaI HpaII KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsaI RsrII SacI
SacII SalI Sau96I SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 1145 CGCG CG^CG
AhdI 910 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 5 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 910 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1271 GGCACC G^G[CT][GA]CC
BsaJI 1277 CCAAGG C^C[ACGT][ACGT]GG
BsiYI 824 CCAGTAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 824 CCAGTAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 1070 TCATGA T^CATGA
BsrDI 1290 CATTGC GCAATG(2/0)
Bst1107I 727 GTATAC GTA^TAC
BstEII 302 GGTTACC G^GT[ACGT]ACC
BstPI 302 GGTTACC G^GT[ACGT]ACC
BstUI 1145 CGCG CG^CG
BstZ17I 727 GTATAC GTA^TAC
DrdI 1060 GACAGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 910 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 667 GAAGAG CTCTTC(1/4)
EcoO65I 302 GGTTACC G^GT[ACGT]ACC
EcoRV 646 GATATC GAT^ATC
EcoT14I 1277 CCAAGG C^C[AT][AT]GG
FauI 1146 GCGGG CCCGC(4/6)
HincII 1228 GTTGAC GT[CT]^[GA]AC
HindII 1228 GTTGAC GT[CT]^[GA]AC
Ksp632I 667 GAAGAG CTCTTC(1/4)
MspA1I 711 CAGCTG C[AC]G^C[GT]G
MvnI 1145 CGCG CG^CG
NlaIV 1271 GGCACC GG[ACGT]^[ACGT]CC
PstI 581 CTGCAG CTGCA^G
PvuII 711 CAGCTG CAG^CTG
RcaI 1070 TCATGA T^CATGA
SapI 667 GAAGAGC GCTCTTC(1/4)
SexAI 968 ACCAGGT A^CC[AT]GGT
SspI 510 AATATT AAT^ATT
StyI 1277 CCAAGG C^C[AT][AT]GG
TaqI 891 TCGA T^CGA
Tsp45I 761 GTGAC ^GT[GC]AC
TthHB8I 891 TCGA T^CGA
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 336 GTCTAC GT^[AC][GT]AC
727 GTATAC
AciI 594 GCGG CCGC(-3/-1)
1146 GCGG
AseI 553 ATTAAT AT^TAAT
598 ATTAAT
AsnI 553 ATTAAT AT^TAAT
598 ATTAAT
BpmI 11 CTGGAG CTGGAG(16/14)
116 CTGGAG
BsmFI 380 GGGAC GGGAC(10/14)
932 GGGAC
BssKI 969 CCAGG ^CC[ACGT]GG
1268 CCTGG
BstNI 969 CCAGG CC^[AT]GG
1268 CCTGG
Cac8I 654 GCCTGC GC[ACGT]^[ACGT]GC
1202 GCAAGC
EcoRII 969 CCAGG ^CC[AT]GG
1268 CCTGG
HphI 48 GGTGA GGTGA(8/7)
681 GGTGA
MvaI 969 CCAGG CC^[AT]GG
1268 CCTGG
PshBI 553 ATTAAT AT^TAAT
598 ATTAAT
ScrFI 969 CCAGG CC^[ACGT]GG
1268 CCTGG
StuI 472 AGGCCT AGG^CCT
952 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BfaI BseRI BsmAI BsrI BstF5I
BstYI DdeI DpnII Fnu4HI FokI
HaeIII ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI NdeII
NlaIII Sau3AI SfaNI SfcI Tru9I
TseI Tsp509I TspRI XhoII