Restriction map for
pF1KB4784
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaAI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MroI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 778 GACGTC GACGT^C
AccI 781 GTCTAC GT^[AC][GT]AC
AfaI 237 GTAC GT^AC
AgeI 533 ACCGGT A^CCGGT
Asp700I 472 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 679 CCCGAG C^[CT]CG[GA]G
BbsI 37 GAAGAC GAAGAC(2/6)
BbvCI 606 CCTCAGC CCTCAGC(-5/-2)
BcnI 549 CCGGG CC^[GC]GG
BglI 490 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 762 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 762 GCTGAGC GC^T[ACGT]AGC
BpuAI 37 GAAGAC GAAGAC(2/6)
BsaBI 744 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 69 GGTCTC GGTCTC(1/5)
BsaWI 533 ACCGGT [AT]^CCGG[AT]
BseRI 757 GAGGAG GAGGAG(10/8)
BsgI 697 GTGCAG GTGCAG(16/14)
BsmBI 450 GAGACG CGTCTC(1/5)
BsmI 684 GAATGC GAATGC(1/-1)
BsoBI 679 CCCGAG C^[CT]CG[GA]G
BsrBI 765 GAGCGG CCGCTC(-3/-3)
BsrDI 204 CATTGC GCAATG(2/0)
BsrFI 533 ACCGGT [GA]^CCGG[CT]
BssSI 95 CTCGTG CACGAG(-5/-1)
BstXI 141 CCAGCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 762 GCTGAGC GC^T[ACGT]AGC
Cfr10I 533 ACCGGT [GA]^CCGG[CT]
DraIII 406 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 481 CTCTTC CTCTTC(1/4)
EcoT14I 792 CCTTGG C^C[AT][AT]GG
HinfI 568 GAGTC G^A[ACGT]TC
Ksp632I 481 CTCTTC CTCTTC(1/4)
MamI 744 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 350 TTAA T^TAA
MspA1I 799 CAGCTG C[AC]G^C[GT]G
NciI 549 CCGGG CC^[GC]GG
NspI 144 GCATGT [GA]CATG^[CT]
PinAI 533 ACCGGT A^CCGGT
PleI 568 GAGTC GAGTC(4/5)
PvuII 799 CAGCTG CAG^CTG
RsaI 237 GTAC GT^AC
SacI 172 GAGCTC GAGCT^C
SexAI 407 ACCAGGT A^CC[AT]GGT
SfcI 383 CTATAG C^T[GA][CT]AG
SmlI 421 CTCAAG C^T[CT][GA]AG
StyI 792 CCTTGG C^C[AT][AT]GG
TaqI 323 TCGA T^CGA
Tru9I 350 TTAA T^TAA
TthHB8I 323 TCGA T^CGA
XcmI 56 CCAAGCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 472 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 29 CGCG CG^CG
136 CGCG
AcyI 40 GACGCC G[GA]^CG[CT]C
778 GACGTC
AlwNI 689 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
722 CAGACACTG
ApaI 551 GGGCCC GGGCC^C
806 GGGCCC
BalI 150 TGGCCA TGG^CCA
497 TGGCCA
BpmI 223 CTCCAG CTGGAG(16/14)
232 CTCCAG
BsaHI 40 GACGCC G[GA]^CG[CT]C
778 GACGTC
BspMI 262 ACCTGC ACCTGC(4/8)
524 ACCTGC
BstF5I 642 CATCC GGATG(2/0)
438 GGATG
BstUI 29 CGCG CG^CG
136 CGCG
CfoI 28 GCGC GCG^C
137 GCGC
EaeI 150 TGGCCA [CT]^GGCC[GA]
497 TGGCCA
FauI 554 CCCGC CCCGC(4/6)
804 GCGGG
FokI 642 CATCC GGATG(9/13)
438 GGATG
HapII 534 CCGG C^CGG
549 CCGG
HgaI 40 GACGC GACGC(5/10)
452 GACGC
HhaI 28 GCGC GCG^C
137 GCGC
Hin1I 40 GACGCC G[GA]^CG[CT]C
778 GACGTC
HinP1I 28 GCGC G^CGC
137 GCGC
HpaII 534 CCGG C^CGG
549 CCGG
MluNI 150 TGGCCA TGG^CCA
497 TGGCCA
MscI 150 TGGCCA TGG^CCA
497 TGGCCA
MspI 534 CCGG C^CGG
549 CCGG
MvnI 29 CGCG CG^CG
136 CGCG
StuI 63 AGGCCT AGG^CCT
427 AGGCCT
Tsp45I 280 GTGAC ^GT[GC]AC
517 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI HaeIII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MvaI MwoI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
TseI TspRI XhoII