Restriction map for
pF1KA0189
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AccI AclI AcsI
AgeI AhdI ApoI AscI AseI
AsnI Asp718I AspEI BalI BciVI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MluNI MscI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 2835 TCCGGA T^CCGGA
AflII 2103 CTTAAG C^TTAAG
AflIII 941 ACGTGT A^C[GA][CT]GT
Alw44I 655 GTGCAC G^TGCAC
Aor51HI 2498 AGCGCT AGC^GCT
ApaLI 655 GTGCAC G^TGCAC
Asp700I 2322 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1882 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1787 TGCGCA TGC^GCA
AvrII 2257 CCTAGG C^CTAGG
BbsI 61 GAAGAC GAAGAC(2/6)
BcgI 213 CGAGCTTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2306 TGATCA T^GATCA
BfrI 2103 CTTAAG C^TTAAG
BglII 1997 AGATCT A^GATCT
BlnI 2257 CCTAGG C^CTAGG
BpuAI 61 GAAGAC GAAGAC(2/6)
BsaBI 2662 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2835 TCCGGA T^CCGGA
BsmI 1763 GCATTC GAATGC(1/-1)
BspEI 2835 TCCGGA T^CCGGA
BspHI 1664 TCATGA T^CATGA
BsrDI 2112 CATTGC GCAATG(2/0)
BssSI 2798 CTCGTG CACGAG(-5/-1)
Eco47III 2498 AGCGCT AGC^GCT
FbaI 2306 TGATCA T^GATCA
FspI 1787 TGCGCA TGC^GCA
MamI 2662 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2835 TCCGGA T^CCGGA
MseI 2104 TTAA T^TAA
PaeR7I 300 CTCGAG C^TCGAG
PflFI 1882 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1664 TCATGA T^CATGA
SacI 2383 GAGCTC GAGCT^C
SapI 76 GAAGAGC GCTCTTC(1/4)
SfiI 588 GGCCTGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 2104 TTAA T^TAA
Tsp509I 13 AATT ^AATT
Tth111I 1882 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 754 CCAGGTAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 300 CTCGAG C^TCGAG
XmnI 2322 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 779 GGCGCC G[GA]^CG[CT]C
2166 GGCGCC
AocI 721 CCTGAGG CC^T[ACGT]AGG
2949 CCTCAGG
AspHI 655 GTGCAC G[AT]GC[AT]^C
2383 GAGCTC
BamHI 1844 GGATCC G^GATCC
2832 GGATCC
BbeI 779 GGCGCC GGCGC^C
2166 GGCGCC
BbrPI 940 CACGTG CAC^GTG
1729 CACGTG
BsaHI 779 GGCGCC G[GA]^CG[CT]C
2166 GGCGCC
BsaWI 2562 ACCGGA [AT]^CCGG[AT]
2835 TCCGGA
BsiHKAI 655 GTGCAC G[AT]GC[AT]^C
2383 GAGCTC
BsrBI 2916 CCGCTC CCGCTC(-3/-3)
2647 GAGCGG
BstXI 2597 CCACAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3002 CCATGGAAGTGG
Bsu36I 721 CCTGAGG CC^T[ACGT]AGG
2949 CCTCAGG
DsaI 1104 CCATGG C^C[GA][CT]GG
3002 CCATGG
Eco81I 721 CCTGAGG CC^T[ACGT]AGG
2949 CCTCAGG
Hin1I 779 GGCGCC G[GA]^CG[CT]C
2166 GGCGCC
KasI 779 GGCGCC G^GCGCC
2166 GGCGCC
NarI 779 GGCGCC GG^CGCC
2166 GGCGCC
NcoI 1104 CCATGG C^CATGG
3002 CCATGG
PmaCI 940 CACGTG CAC^GTG
1729 CACGTG
PmlI 940 CACGTG CAC^GTG
1729 CACGTG
SbfI 1469 CCTGCAGG CCTGCA^GG
3039 CCTGCAGG
SfoI 779 GGCGCC GGC^GCC
2166 GGCGCC
Sse8387I 1469 CCTGCAGG CCTGCA^GG
3039 CCTGCAGG
StuI 587 AGGCCT AGG^CCT
596 AGGCCT
TaqI 22 TCGA T^CGA
301 TCGA
TfiI 400 GAATC G^A[AT]TC
1438 GATTC
TthHB8I 22 TCGA T^CGA
301 TCGA
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TseI Tsp45I TspRI
Van91I XhoII