Restriction map for
pF1KA0160
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I AlwNI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HgaI HincII
HindII HpaI KpnI MfeI MluI
MroI MunI NaeI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SalI SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI TspRI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AcyI 3 GGCGCC G[GA]^CG[CT]C
AfaI 1358 GTAC GT^AC
AflIII 350 ACATGT A^C[GA][CT]GT
AgeI 207 ACCGGT A^CCGGT
AocI 1507 CCTCAGG CC^T[ACGT]AGG
ApaI 43 GGGCCC GGGCC^C
AseI 796 ATTAAT AT^TAAT
AsnI 796 ATTAAT AT^TAAT
Asp700I 832 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 229 GAGCAC G[AT]GC[AT]^C
AvaI 39 CTCGGG C^[CT]CG[GA]G
BalI 1677 TGGCCA TGG^CCA
BamHI 1761 GGATCC G^GATCC
BanII 43 GGGCCC G[GA]GC[CT]^C
BbeI 3 GGCGCC GGCGC^C
BciVI 1248 GGATAC GTATCC(6/5)
BsaBI 1513 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsaI 1090 GAGACC GGTCTC(1/5)
BsaWI 207 ACCGGT [AT]^CCGG[AT]
BsgI 1590 GTGCAG GTGCAG(16/14)
BsiHKAI 229 GAGCAC G[AT]GC[AT]^C
BsoBI 39 CTCGGG C^[CT]CG[GA]G
BspHI 1862 TCATGA T^CATGA
BspLU11I 350 ACATGT A^CATGT
BsrDI 1345 CATTGC GCAATG(2/0)
BsrFI 207 ACCGGT [GA]^CCGG[CT]
BssSI 247 CACGAG CACGAG(-5/-1)
Bsu36I 1507 CCTCAGG CC^T[ACGT]AGG
Cfr10I 207 ACCGGT [GA]^CCGG[CT]
DraI 394 TTTAAA TTT^AAA
DsaI 176 CCGCGG C^C[GA][CT]GG
EaeI 1677 TGGCCA [CT]^GGCC[GA]
Eco81I 1507 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1351 CCTTGG C^C[AT][AT]GG
EcoT22I 1987 ATGCAT ATGCA^T
Hin1I 3 GGCGCC G[GA]^CG[CT]C
HindIII 2159 AAGCTT A^AGCTT
KasI 3 GGCGCC G^GCGCC
KspI 176 CCGCGG CCGC^GG
MamI 1513 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1677 TGGCCA TGG^CCA
MscI 1677 TGGCCA TGG^CCA
NarI 3 GGCGCC GG^CGCC
NdeI 727 CATATG CA^TATG
NsiI 1987 ATGCAT ATGCA^T
PinAI 207 ACCGGT A^CCGGT
PpuMI 1053 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 796 ATTAAT AT^TAAT
RcaI 1862 TCATGA T^CATGA
RsaI 1358 GTAC GT^AC
SacII 176 CCGCGG CCGC^GG
SapI 832 GAAGAGC GCTCTTC(1/4)
SfaNI 1395 GCATC GCATC(5/9)
SfoI 3 GGCGCC GGC^GCC
SmlI 1390 CTCAAG C^T[CT][GA]AG
SphI 1985 GCATGC GCATG^C
StuI 263 AGGCCT AGG^CCT
StyI 1351 CCTTGG C^C[AT][AT]GG
Tsp45I 760 GTGAC ^GT[GC]AC
XcmI 953 CCATGCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 832 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 51 CGCG CG^CG
177 CGCG
AlwI 1762 GATCC GGATC(4/5)
1761 GGATC
BanI 3 GGCGCC G^G[CT][GA]CC
636 GGTGCC
BcnI 59 CCGGG CC^[GC]GG
670 CCCGG
BglII 284 AGATCT A^GATCT
1224 AGATCT
BmyI 43 GGGCCC G[AGT]GC[ACT]^C
229 GAGCAC
BpmI 259 CTCCAG CTGGAG(16/14)
366 CTCCAG
Bsp1286I 43 GGGCCC G[AGT]GC[ACT]^C
229 GAGCAC
BstBI 299 TTCGAA TT^CGAA
1036 TTCGAA
BstUI 51 CGCG CG^CG
177 CGCG
DraII 42 GGGGCCC [GA]G^G[ACGT]CC[CT]
1053 GGGACCT
EcoO109I 42 GGGGCCC [GA]G^G[ACGT]CC[CT]
1053 GGGACCT
HaeII 3 GGCGCC [GA]GCGC^[CT]
917 GGCGCT
MslI 16 CACGGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
496 CACAAAAATG
MspA1I 176 CCGCGG C[AC]G^C[GT]G
185 CAGCGG
MvnI 51 CGCG CG^CG
177 CGCG
NciI 59 CCGGG CC^[GC]GG
670 CCCGG
NspV 299 TTCGAA TT^CGAA
1036 TTCGAA
PleI 762 GACTC GAGTC(4/5)
1149 GAGTC
SfcI 288 CTATAG C^T[GA][CT]AG
1220 CTACAG
SfuI 299 TTCGAA TT^CGAA
1036 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BfaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI Sau3AI Sau96I
ScrFI SspI TaqI TfiI Tru9I
TseI Tsp509I TthHB8I XhoII