Restriction map for
pF1KF0229
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BbrPI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BsrBI
BssSI Bst1107I BstAPI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FokI FseI FspI
KpnI KspI MfeI MluI MroI
MspA1I MunI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI Tsp45I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1663 GTAGAC GT^[AC][GT]AC
AflIII 712 ACATGT A^C[GA][CT]GT
AlwNI 917 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 109 GGCGCGCC GG^CGCGCC
AspI 1666 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1468 GGACC G^G[AT]CC
BalI 1093 TGGCCA TGG^CCA
BbsI 360 GTCTTC GAAGAC(2/6)
BcgI 1698 GCAGGCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 524 TGATCA T^GATCA
BpuAI 360 GTCTTC GAAGAC(2/6)
BsaBI 628 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1115 GAGACC GGTCTC(1/5)
BseRI 369 GAGGAG GAGGAG(10/8)
BsmI 252 GCATTC GAATGC(1/-1)
Bsp1407I 1266 TGTACA T^GTACA
BspDI 635 ATCGAT AT^CGAT
BspLU11I 712 ACATGT A^CATGT
BsrDI 1778 CATTGC GCAATG(2/0)
BsrGI 1266 TGTACA T^GTACA
BssHII 110 GCGCGC G^CGCGC
BstBI 1106 TTCGAA TT^CGAA
ClaI 635 ATCGAT AT^CGAT
DraI 1224 TTTAAA TTT^AAA
EcoRI 1003 GAATTC G^AATTC
FbaI 524 TGATCA T^GATCA
HgaI 1310 GACGC GACGC(5/10)
HindIII 1368 AAGCTT A^AGCTT
MaeII 1278 ACGT A^CGT
MamI 628 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1093 TGGCCA TGG^CCA
MscI 1093 TGGCCA TGG^CCA
NaeI 87 GCCGGC GCC^GGC
NcoI 1399 CCATGG C^CATGG
NgoMIV 87 GCCGGC G^CCGGC
NspI 712 ACATGT [GA]CATG^[CT]
NspV 1106 TTCGAA TT^CGAA
PflFI 1666 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1467 AGGACCT [GA]G^G[AT]CC[CT]
ScaI 959 AGTACT AGT^ACT
SfcI 1127 CTACAG C^T[GA][CT]AG
SfuI 1106 TTCGAA TT^CGAA
SmlI 1456 CTTGAG C^T[CT][GA]AG
SspBI 1266 TGTACA T^GTACA
SspI 283 AATATT AAT^ATT
SwaI 1223 ATTTAAAT ATTT^AAAT
Tth111I 1666 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 79 CGCG CG^CG
111 CGCG
AcyI 84 GGCGCC G[GA]^CG[CT]C
894 GGCGCC
ApaI 12 GGGCCC GGGCC^C
28 GGGCCC
AspHI 562 GTGCTC G[AT]GC[AT]^C
1711 GAGCAC
BamHI 435 GGATCC G^GATCC
1634 GGATCC
BanI 84 GGCGCC G^G[CT][GA]CC
894 GGCGCC
BbeI 84 GGCGCC GGCGC^C
894 GGCGCC
BbvCI 247 CCTCAGC CCTCAGC(-5/-2)
1471 CCTCAGC
BfaI 1484 CTAG C^TAG
1600 CTAG
BglI 14 GCCCTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
91 GCCCTTGTGGC
BsaHI 84 GGCGCC G[GA]^CG[CT]C
894 GGCGCC
BsgI 184 CTGCAC GTGCAG(16/14)
745 GTGCAG
BsiHKAI 562 GTGCTC G[AT]GC[AT]^C
1711 GAGCAC
BsmFI 164 GGGAC GGGAC(10/14)
730 GGGAC
BsrFI 87 GCCGGC [GA]^CCGG[CT]
1514 ACCGGC
BstUI 79 CGCG CG^CG
111 CGCG
BstYI 435 GGATCC [GA]^GATC[CT]
1634 GGATCC
Cfr10I 87 GCCGGC [GA]^CCGG[CT]
1514 ACCGGC
DraIII 187 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
556 CACACTGTG
EcoT14I 515 CCTTGG C^C[AT][AT]GG
1399 CCATGG
FauI 19 GCGGG CCCGC(4/6)
103 GCGGG
HaeII 84 GGCGCC [GA]GCGC^[CT]
894 GGCGCC
Hin1I 84 GGCGCC G[GA]^CG[CT]C
894 GGCGCC
HincII 750 GTTAAC GT[CT]^[GA]AC
937 GTTAAC
HindII 750 GTTAAC GT[CT]^[GA]AC
937 GTTAAC
HpaI 750 GTTAAC GTT^AAC
937 GTTAAC
HphI 1132 GGTGA GGTGA(8/7)
901 TCACC
KasI 84 GGCGCC G^GCGCC
894 GGCGCC
MaeI 1484 CTAG C^TAG
1600 CTAG
MaeIII 1057 GTAAC ^GT[ACGT]AC
1746 GTTAC
MflI 435 GGATCC [GA]^GATC[CT]
1634 GGATCC
MvnI 79 CGCG CG^CG
111 CGCG
NarI 84 GGCGCC GG^CGCC
894 GGCGCC
PflMI 225 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1578 CCAAAAATTGG
PleI 1179 GACTC GAGTC(4/5)
1407 GAGTC
SfoI 84 GGCGCC GGC^GCC
894 GGCGCC
StyI 515 CCTTGG C^C[AT][AT]GG
1399 CCATGG
Van91I 225 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1578 CCAAAAATTGG
XbaI 1483 TCTAGA T^CTAGA
1599 TCTAGA
XhoII 435 GGATCC [GA]^GATC[CT]
1634 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
Bsp1286I BspHI BspMI BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I