Restriction map for
pF1KSDB0050
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
Aor51HI ApaLI AseI AsnI AspEI
AspI AviII BamHI BcgI BsiWI
BsmI BspDI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 2003 GGTACC G^GTACC
AccI 2872 GTCTAC GT^[AC][GT]AC
AccIII 470 TCCGGA T^CCGGA
AflII 1363 CTTAAG C^TTAAG
AscI 43 GGCGCGCC GG^CGCGCC
Asp718I 2003 GGTACC G^GTACC
AvrII 2601 CCTAGG C^CTAGG
BalI 3418 TGGCCA TGG^CCA
BbrPI 180 CACGTG CAC^GTG
BclI 3617 TGATCA T^GATCA
BfrI 1363 CTTAAG C^TTAAG
BglI 1201 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2601 CCTAGG C^CTAGG
BseAI 470 TCCGGA T^CCGGA
BsmBI 166 GAGACG CGTCTC(1/5)
Bsp1407I 662 TGTACA T^GTACA
BspEI 470 TCCGGA T^CCGGA
BspMI 1920 GCAGGT ACCTGC(4/8)
BsrGI 662 TGTACA T^GTACA
BssHII 44 GCGCGC G^CGCGC
BstEII 3652 GGTGACC G^GT[ACGT]ACC
BstPI 3652 GGTGACC G^GT[ACGT]ACC
DrdI 1873 GACAACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3652 GGTGACC G^GT[ACGT]ACC
FbaI 3617 TGATCA T^GATCA
KpnI 2003 GGTACC GGTAC^C
KspI 3246 CCGCGG CCGC^GG
MluNI 3418 TGGCCA TGG^CCA
MroI 470 TCCGGA T^CCGGA
MscI 3418 TGGCCA TGG^CCA
NdeI 2102 CATATG CA^TATG
PaeR7I 85 CTCGAG C^TCGAG
PmaCI 180 CACGTG CAC^GTG
PmlI 180 CACGTG CAC^GTG
SacI 2854 GAGCTC GAGCT^C
SacII 3246 CCGCGG CCGC^GG
SapI 2679 GCTCTTC GCTCTTC(1/4)
SmaI 3731 CCCGGG CCC^GGG
SphI 1197 GCATGC GCATG^C
SspBI 662 TGTACA T^GTACA
XcmI 3711 CCAGCTGTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 85 CTCGAG C^TCGAG
XmaCI 3731 CCCGGG C^CCGGG
XmaI 3731 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 2659 CCTGAGG CC^T[ACGT]AGG
3704 CCTCAGG
Asp700I 144 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1644 GAAGTGGTTC
BbeI 7 GGCGCC GGCGC^C
193 GGCGCC
BbvCI 1399 GCTGAGG CCTCAGC(-5/-2)
2122 GCTGAGG
BglII 1328 AGATCT A^GATCT
1547 AGATCT
BsaWI 470 TCCGGA [AT]^CCGG[AT]
1726 ACCGGA
BsiEI 298 CGGCCG CG[GA][CT]^CG
727 CGGCCG
BspHI 101 TCATGA T^CATGA
3161 TCATGA
BstXI 1316 CCAAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2693 CCATCAGCATGG
Bsu36I 2659 CCTGAGG CC^T[ACGT]AGG
3704 CCTCAGG
EagI 298 CGGCCG C^GGCCG
727 CGGCCG
EclXI 298 CGGCCG C^GGCCG
727 CGGCCG
Eco52I 298 CGGCCG C^GGCCG
727 CGGCCG
Eco81I 2659 CCTGAGG CC^T[ACGT]AGG
3704 CCTCAGG
EcoNI 2319 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3516 CCTGGCTCAGG
KasI 7 GGCGCC G^GCGCC
193 GGCGCC
NaeI 2634 GCCGGC GCC^GGC
2879 GCCGGC
NarI 7 GGCGCC GG^CGCC
193 GGCGCC
NcoI 1137 CCATGG C^CATGG
1653 CCATGG
NgoMIV 2634 GCCGGC G^CCGGC
2879 GCCGGC
RcaI 101 TCATGA T^CATGA
3161 TCATGA
ScaI 706 AGTACT AGT^ACT
1712 AGTACT
SfoI 7 GGCGCC GGC^GCC
193 GGCGCC
SmlI 85 CTCGAG C^T[CT][GA]AG
1363 CTTAAG
StuI 3215 AGGCCT AGG^CCT
3543 AGGCCT
XmnI 144 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1644 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII