Restriction map for
pF1KSDB0014
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# NO CUT ENZYMES ( 58 enzymes) #
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Acc65I AccI AccIII AclI AgeI
AocI AscI AseI AsnI Asp718I
AvrII BciVI BlnI BsaWI BseAI
BspDI BspEI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI DraI DraIII
Eco81I EcoRV EcoT22I FseI HindIII
HpaI KpnI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI SalI
ScaI SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 2341 GACGTC GACGT^C
AflII 5368 CTTAAG C^TTAAG
Alw44I 513 GTGCAC G^TGCAC
ApaLI 513 GTGCAC G^TGCAC
Asp700I 4014 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3817 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 267 CACGTG CAC^GTG
BfrI 5368 CTTAAG C^TTAAG
BlpI 3972 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3972 GCTGAGC GC^T[ACGT]AGC
BsaBI 19 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 292 CTGCAC GTGCAG(16/14)
BsiWI 575 CGTACG C^GTACG
Bsp1407I 410 TGTACA T^GTACA
BspHI 2225 TCATGA T^CATGA
BspLU11I 950 ACATGT A^CATGT
BsrGI 410 TGTACA T^GTACA
CelII 3972 GCTGAGC GC^T[ACGT]AGC
CpoI 5515 CGGACCG CG^G[AT]CCG
EagI 5717 CGGCCG C^GGCCG
EclXI 5717 CGGCCG C^GGCCG
Eco52I 5717 CGGCCG C^GGCCG
EcoRI 4014 GAATTC G^AATTC
HincII 4678 GTCAAC GT[CT]^[GA]AC
HindII 4678 GTCAAC GT[CT]^[GA]AC
KspI 5072 CCGCGG CCGC^GG
MamI 19 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 5369 TTAA T^TAA
PflFI 3817 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 267 CACGTG CAC^GTG
PmlI 267 CACGTG CAC^GTG
RcaI 2225 TCATGA T^CATGA
RsrII 5515 CGGACCG CG^G[AT]CCG
SacII 5072 CCGCGG CCGC^GG
SexAI 1865 ACCAGGT A^CC[AT]GGT
SphI 4949 GCATGC GCATG^C
SspBI 410 TGTACA T^GTACA
Tru9I 5369 TTAA T^TAA
Tth111I 3817 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4014 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 709 AAATTC [GA]^AATT[CT]
4014 GAATTC
AflIII 918 ACACGT A^C[GA][CT]GT
950 ACATGT
Aor51HI 2786 AGCGCT AGC^GCT
4811 AGCGCT
ApoI 709 AAATTC [GA]^AATT[CT]
4014 GAATTC
AviII 1292 TGCGCA TGC^GCA
3800 TGCGCA
BamHI 1346 GGATCC G^GATCC
1767 GGATCC
BbeI 1330 GGCGCC GGCGC^C
5812 GGCGCC
BcgI 72 CGACTGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2679 CGAGCTGTGTGC
BclI 3083 TGATCA T^GATCA
5240 TGATCA
BsaAI 267 CACGTG [CT]AC^GT[GA]
577 TACGTG
BsmBI 2614 GAGACG CGTCTC(1/5)
5713 GAGACG
BsmI 5331 GAATGC GAATGC(1/-1)
1568 GCATTC
BsrDI 1356 CATTGC GCAATG(2/0)
3079 GCAATG
BstEII 2898 GGTGACC G^GT[ACGT]ACC
3822 GGTCACC
BstPI 2898 GGTGACC G^GT[ACGT]ACC
3822 GGTCACC
DrdI 1813 GACAAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2616 GACGCTGCAGTC
Eco47III 2786 AGCGCT AGC^GCT
4811 AGCGCT
EcoO65I 2898 GGTGACC G^GT[ACGT]ACC
3822 GGTCACC
FbaI 3083 TGATCA T^GATCA
5240 TGATCA
FspI 1292 TGCGCA TGC^GCA
3800 TGCGCA
KasI 1330 GGCGCC G^GCGCC
5812 GGCGCC
NarI 1330 GGCGCC GG^CGCC
5812 GGCGCC
NcoI 965 CCATGG C^CATGG
4463 CCATGG
SfoI 1330 GGCGCC GGC^GCC
5812 GGCGCC
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# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SfiI SmaI SmlI
Sse8387I StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI