Restriction map for
pF1KSDA1819
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# NO CUT ENZYMES ( 87 enzymes) #
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Acc65I AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BbeI BbrPI
BcgI BfrI BglII BsaAI BsaBI
BsiEI BsiWI BspDI BspHI BsrBI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoT22I FspI KasI KpnI
Ksp632I KspI MamI MluI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 2928 GACGTC GACGT^C
AccI 2538 GTCTAC GT^[AC][GT]AC
AccII 2331 CGCG CG^CG
AccIII 1252 TCCGGA T^CCGGA
AflIII 1538 ACATGT A^C[GA][CT]GT
Alw44I 94 GTGCAC G^TGCAC
ApaLI 94 GTGCAC G^TGCAC
AvaI 87 CCCGAG C^[CT]CG[GA]G
AvrII 1057 CCTAGG C^CTAGG
BamHI 906 GGATCC G^GATCC
BanI 1571 GGCACC G^G[CT][GA]CC
BbsI 3390 GAAGAC GAAGAC(2/6)
BlnI 1057 CCTAGG C^CTAGG
BpuAI 3390 GAAGAC GAAGAC(2/6)
BsaWI 1252 TCCGGA [AT]^CCGG[AT]
BseAI 1252 TCCGGA T^CCGGA
BsgI 2876 CTGCAC GTGCAG(16/14)
BsoBI 87 CCCGAG C^[CT]CG[GA]G
BspEI 1252 TCCGGA T^CCGGA
BspLU11I 1538 ACATGT A^CATGT
BsrFI 1549 GCCGGC [GA]^CCGG[CT]
BstEII 2033 GGTCACC G^GT[ACGT]ACC
BstPI 2033 GGTCACC G^GT[ACGT]ACC
BstUI 2331 CGCG CG^CG
Cfr10I 1549 GCCGGC [GA]^CCGG[CT]
DraIII 2137 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2033 GGTCACC G^GT[ACGT]ACC
EcoRI 614 GAATTC G^AATTC
EcoRV 604 GATATC GAT^ATC
FseI 1548 GGCCGGCC GGCCGG^CC
HindIII 2463 AAGCTT A^AGCTT
MroI 1252 TCCGGA T^CCGGA
MvnI 2331 CGCG CG^CG
NaeI 1549 GCCGGC GCC^GGC
NgoMIV 1549 GCCGGC G^CCGGC
SexAI 1413 ACCAGGT A^CC[AT]GGT
SmlI 2894 CTCAAG C^T[CT][GA]AG
SspI 859 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcsI 614 GAATTC [GA]^AATT[CT]
2805 AAATTT
AcyI 2928 GACGTC G[GA]^CG[CT]C
3237 GACGCC
ApaI 1122 GGGCCC GGGCC^C
3345 GGGCCC
ApoI 614 GAATTC [GA]^AATT[CT]
2805 AAATTT
AspI 671 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
813 GACTCTGTC
BciVI 2101 GGATAC GTATCC(6/5)
1288 GTATCC
BlpI 1234 GCTCAGC GC^T[ACGT]AGC
3150 GCTAAGC
Bpu1102I 1234 GCTCAGC GC^T[ACGT]AGC
3150 GCTAAGC
BsaHI 2928 GACGTC G[GA]^CG[CT]C
3237 GACGCC
BsaI 2799 GAGACC GGTCTC(1/5)
3069 GAGACC
BsmBI 1202 CGTCTC CGTCTC(1/5)
881 GAGACG
BsmI 2558 GAATGC GAATGC(1/-1)
1168 GCATTC
Bsp1407I 237 TGTACA T^GTACA
1541 TGTACA
BspMI 410 ACCTGC ACCTGC(4/8)
2961 ACCTGC
BsrDI 2088 CATTGC GCAATG(2/0)
761 GCAATG
BsrGI 237 TGTACA T^GTACA
1541 TGTACA
BstAPI 405 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2313 GCAGCAGATGC
CelII 1234 GCTCAGC GC^T[ACGT]AGC
3150 GCTAAGC
EcoNI 1240 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2725 CCTACCTTAGG
HaeII 12 AGCGCC [GA]GCGC^[CT]
113 AGCGCC
HgaI 2329 GACGC GACGC(5/10)
3237 GACGC
Hin1I 2928 GACGTC G[GA]^CG[CT]C
3237 GACGCC
HincII 544 GTTAAC GT[CT]^[GA]AC
3048 GTTAAC
HindII 544 GTTAAC GT[CT]^[GA]AC
3048 GTTAAC
HpaI 544 GTTAAC GTT^AAC
3048 GTTAAC
MaeII 2852 ACGT A^CGT
2929 ACGT
PflFI 671 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
813 GACTCTGTC
PpuMI 691 GGGACCT [GA]G^G[AT]CC[CT]
3114 GGGTCCC
PshAI 1027 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2366 GACATTTGTC
PvuII 1141 CAGCTG CAG^CTG
1157 CAGCTG
SphI 1335 GCATGC GCATG^C
2828 GCATGC
SspBI 237 TGTACA T^GTACA
1541 TGTACA
Tth111I 671 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
813 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaII BalI BanII
BbvCI BbvI BclI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII