Restriction map for
pF1KB5674
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI AlwNI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DsaI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FauI FbaI FseI
HgaI HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 142 GACGTC GACGT^C
AcyI 142 GACGTC G[GA]^CG[CT]C
AfaI 135 GTAC GT^AC
AflIII 1235 ACATGT A^C[GA][CT]GT
Alw44I 637 GTGCAC G^TGCAC
ApaLI 637 GTGCAC G^TGCAC
Asp700I 1218 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 361 TGCGCA TGC^GCA
BglII 509 AGATCT A^GATCT
BsaHI 142 GACGTC G[GA]^CG[CT]C
BsaI 1009 GAGACC GGTCTC(1/5)
BsiEI 1259 CGGCCG CG[GA][CT]^CG
BsmBI 140 GAGACG CGTCTC(1/5)
BspLU11I 1235 ACATGT A^CATGT
BsrFI 1261 GCCGGC [GA]^CCGG[CT]
BssSI 886 CACGAG CACGAG(-5/-1)
BstF5I 770 GGATG GGATG(2/0)
BstYI 509 AGATCT [GA]^GATC[CT]
Cfr10I 1261 GCCGGC [GA]^CCGG[CT]
DrdI 541 GACTTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1259 CGGCCG C^GGCCG
EclXI 1259 CGGCCG C^GGCCG
Eco52I 1259 CGGCCG C^GGCCG
FokI 770 GGATG GGATG(9/13)
FspI 361 TGCGCA TGC^GCA
HaeII 1264 GGCGCT [GA]GCGC^[CT]
Hin1I 142 GACGTC G[GA]^CG[CT]C
HincII 1231 GTCAAC GT[CT]^[GA]AC
HindII 1231 GTCAAC GT[CT]^[GA]AC
HindIII 106 AAGCTT A^AGCTT
MaeII 143 ACGT A^CGT
MflI 509 AGATCT [GA]^GATC[CT]
MspA1I 796 CAGCTG C[AC]G^C[GT]G
NaeI 1261 GCCGGC GCC^GGC
NgoMIV 1261 GCCGGC G^CCGGC
PaeR7I 503 CTCGAG C^TCGAG
PflMI 955 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 890 AGGACCT [GA]G^G[AT]CC[CT]
PstI 642 CTGCAG CTGCA^G
PvuII 796 CAGCTG CAG^CTG
RsaI 135 GTAC GT^AC
SmlI 503 CTCGAG C^T[CT][GA]AG
Van91I 955 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 503 CTCGAG C^TCGAG
XhoII 509 AGATCT [GA]^GATC[CT]
XmnI 1218 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 414 AAATTT [GA]^AATT[CT]
835 AAATTC
AlwI 780 GATCC GGATC(4/5)
864 GGATC
ApaI 255 GGGCCC GGGCC^C
625 GGGCCC
ApoI 414 AAATTT [GA]^AATT[CT]
835 AAATTC
AspHI 619 GAGCAC G[AT]GC[AT]^C
637 GTGCAC
BalI 101 TGGCCA TGG^CCA
373 TGGCCA
BanI 829 GGTGCC G^G[CT][GA]CC
1141 GGTGCC
BbsI 406 GAAGAC GAAGAC(2/6)
1133 GTCTTC
BbvI 798 GCTGC GCAGC(8/12)
1150 GCTGC
BcgI 1194 CGAGGACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
311 GCAGGGGTGTCG
BfaI 206 CTAG C^TAG
472 CTAG
BpmI 895 CTGGAG CTGGAG(16/14)
949 CTGGAG
BpuAI 406 GAAGAC GAAGAC(2/6)
1133 GTCTTC
BsgI 647 GTGCAG GTGCAG(16/14)
859 GTGCAG
BsiHKAI 619 GAGCAC G[AT]GC[AT]^C
637 GTGCAC
BsmAI 140 GAGAC GTCTC(1/5)
1009 GAGAC
BspMI 688 ACCTGC ACCTGC(4/8)
659 GCAGGT
Cac8I 649 GCAGGC GC[ACGT]^[ACGT]GC
1261 GCCGGC
EarI 852 CTCTTC CTCTTC(1/4)
1006 GAAGAG
Ksp632I 852 CTCTTC CTCTTC(1/4)
1006 GAAGAG
MaeI 206 CTAG C^TAG
472 CTAG
MluNI 101 TGGCCA TGG^CCA
373 TGGCCA
MscI 101 TGGCCA TGG^CCA
373 TGGCCA
MslI 640 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1236 CATGTGCGTG
NspI 271 ACATGC [GA]CATG^[CT]
1235 ACATGT
PshAI 142 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
157 GACCATAGTC
SfaNI 653 GCATC GCATC(5/9)
1079 GCATC
SfcI 642 CTGCAG C^T[GA][CT]AG
1278 CTACAG
TfiI 556 GAATC G^A[AT]TC
944 GAATC
TseI 798 GCTGC G^C[AT]GC
1150 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AvaI AvaII BanII
BcnI BmyI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstNI CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoRV
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
TaqI Tru9I Tsp45I Tsp509I TspRI
TthHB8I XcmI