Restriction map for
pF1KB5580
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# NO CUT ENZYMES ( 114 enzymes) #
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Acc65I AccII AclI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbrPI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT14I FbaI FseI
FspI HpaI KasI KpnI KspI
MaeI MamI MluI MvnI NarI
NcoI NdeI NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 376 GACGTC GACGT^C
AccI 1107 GTCGAC GT^[AC][GT]AC
AflIII 1152 ACACGT A^C[GA][CT]GT
Alw44I 1204 GTGCAC G^TGCAC
ApaLI 1204 GTGCAC G^TGCAC
Asp700I 630 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 986 CTCGGG C^[CT]CG[GA]G
AvaII 963 GGACC G^G[AT]CC
BbvCI 58 CCTCAGC CCTCAGC(-5/-2)
BcgI 1251 GCACTGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1679 CCCGG CC^[GC]GG
BglI 1273 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 815 TACGTG [CT]AC^GT[GA]
BsgI 616 GTGCAG GTGCAG(16/14)
BsiEI 432 CGGCCG CG[GA][CT]^CG
BsmI 65 GCATTC GAATGC(1/-1)
BsoBI 986 CTCGGG C^[CT]CG[GA]G
BspHI 1367 TCATGA T^CATGA
BsrDI 1760 GCAATG GCAATG(2/0)
BstEII 1441 GGTGACC G^GT[ACGT]ACC
BstPI 1441 GGTGACC G^GT[ACGT]ACC
DraII 1131 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 842 CCACGG C^C[GA][CT]GG
EagI 432 CGGCCG C^GGCCG
EclXI 432 CGGCCG C^GGCCG
Eco52I 432 CGGCCG C^GGCCG
EcoO109I 1131 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1441 GGTGACC G^GT[ACGT]ACC
EcoT22I 1532 ATGCAT ATGCA^T
FauI 1314 GCGGG CCCGC(4/6)
HaeII 274 AGCGCC [GA]GCGC^[CT]
HgaI 181 GACGC GACGC(5/10)
HindIII 1044 AAGCTT A^AGCTT
MfeI 663 CAATTG C^AATTG
MunI 663 CAATTG C^AATTG
NciI 1679 CCCGG CC^[GC]GG
NsiI 1532 ATGCAT ATGCA^T
PshAI 372 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 510 CAGCTG CAG^CTG
RcaI 1367 TCATGA T^CATGA
SalI 1107 GTCGAC G^TCGAC
SapI 1332 GCTCTTC GCTCTTC(1/4)
ScaI 401 AGTACT AGT^ACT
SmlI 1795 CTCAAG C^T[CT][GA]AG
XmnI 630 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 187 TCCGGA T^CCGGA
695 TCCGGA
AcsI 498 AAATTT [GA]^AATT[CT]
1603 AAATTC
AcyI 181 GACGCC G[GA]^CG[CT]C
376 GACGTC
ApoI 498 AAATTT [GA]^AATT[CT]
1603 AAATTC
BalI 839 TGGCCA TGG^CCA
1821 TGGCCA
BsaHI 181 GACGCC G[GA]^CG[CT]C
376 GACGTC
BsaI 1100 GAGACC GGTCTC(1/5)
1750 GGTCTC
BseAI 187 TCCGGA T^CCGGA
695 TCCGGA
BseRI 1032 CTCCTC GAGGAG(10/8)
1073 GAGGAG
BsmFI 313 GTCCC GGGAC(10/14)
713 GTCCC
BspEI 187 TCCGGA T^CCGGA
695 TCCGGA
BspMI 81 ACCTGC ACCTGC(4/8)
1406 ACCTGC
BsrFI 572 GCCGGC [GA]^CCGG[CT]
1698 GCCGGC
BstXI 731 CCACGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1379 CCACCATCCTGG
CfoI 63 GCGC GCG^C
275 GCGC
Cfr10I 572 GCCGGC [GA]^CCGG[CT]
1698 GCCGGC
EarI 1035 CTCTTC CTCTTC(1/4)
1333 CTCTTC
HhaI 63 GCGC GCG^C
275 GCGC
Hin1I 181 GACGCC G[GA]^CG[CT]C
376 GACGTC
HinP1I 63 GCGC G^CGC
275 GCGC
HincII 1107 GTCGAC GT[CT]^[GA]AC
1399 GTCAAC
HindII 1107 GTCGAC GT[CT]^[GA]AC
1399 GTCAAC
Ksp632I 1035 CTCTTC CTCTTC(1/4)
1333 CTCTTC
MluNI 839 TGGCCA TGG^CCA
1821 TGGCCA
MroI 187 TCCGGA T^CCGGA
695 TCCGGA
MscI 839 TGGCCA TGG^CCA
1821 TGGCCA
NaeI 572 GCCGGC GCC^GGC
1698 GCCGGC
NgoMIV 572 GCCGGC G^CCGGC
1698 GCCGGC
PstI 1408 CTGCAG CTGCA^G
1480 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BamHI BanII BbsI BbvI BmyI
BpuAI BsaJI BsaWI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DrdI EaeI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII