Restriction map for
pF1KB5299
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AocI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BanI BbeI BbrPI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluNI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccI 1013 GTAGAC GT^[AC][GT]AC
AccII 790 CGCG CG^CG
AccIII 1103 TCCGGA T^CCGGA
AcsI 41 GAATTT [GA]^AATT[CT]
AcyI 90 GACGCC G[GA]^CG[CT]C
AfaI 1059 GTAC GT^AC
AflIII 789 ACGCGT A^C[GA][CT]GT
Alw44I 505 GTGCAC G^TGCAC
AlwNI 1318 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 117 AGCGCT AGC^GCT
ApaLI 505 GTGCAC G^TGCAC
ApoI 41 GAATTT [GA]^AATT[CT]
Asp700I 343 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 153 TGCGCA TGC^GCA
BamHI 1113 GGATCC G^GATCC
BcgI 140 CGATCCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1309 CTAG C^TAG
BsaHI 90 GACGCC G[GA]^CG[CT]C
BsaI 957 GGTCTC GGTCTC(1/5)
BseAI 1103 TCCGGA T^CCGGA
BseRI 317 CTCCTC GAGGAG(10/8)
BsmI 1351 GAATGC GAATGC(1/-1)
BspDI 311 ATCGAT AT^CGAT
BspEI 1103 TCCGGA T^CCGGA
BspHI 1277 TCATGA T^CATGA
BspMI 158 ACCTGC ACCTGC(4/8)
BsrBI 540 CCGCTC CCGCTC(-3/-3)
BstAPI 981 GCACGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 548 GGTCACC G^GT[ACGT]ACC
BstPI 548 GGTCACC G^GT[ACGT]ACC
BstUI 790 CGCG CG^CG
BstYI 1113 GGATCC [GA]^GATC[CT]
ClaI 311 ATCGAT AT^CGAT
DraIII 847 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1284 TGGCCG [CT]^GGCC[GA]
EarI 976 GAAGAG CTCTTC(1/4)
Eco47III 117 AGCGCT AGC^GCT
EcoNI 319 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 548 GGTCACC G^GT[ACGT]ACC
EcoT14I 716 CCATGG C^C[AT][AT]GG
FauI 969 CCCGC CCCGC(4/6)
FspI 153 TGCGCA TGC^GCA
HaeII 117 AGCGCT [GA]GCGC^[CT]
Hin1I 90 GACGCC G[GA]^CG[CT]C
Ksp632I 976 GAAGAG CTCTTC(1/4)
MaeI 1309 CTAG C^TAG
MfeI 242 CAATTG C^AATTG
MflI 1113 GGATCC [GA]^GATC[CT]
MluI 789 ACGCGT A^CGCGT
MroI 1103 TCCGGA T^CCGGA
MunI 242 CAATTG C^AATTG
MvnI 790 CGCG CG^CG
NcoI 716 CCATGG C^CATGG
PaeR7I 113 CTCGAG C^TCGAG
PvuII 799 CAGCTG CAG^CTG
RcaI 1277 TCATGA T^CATGA
RsaI 1059 GTAC GT^AC
SacI 650 GAGCTC GAGCT^C
SapI 976 GAAGAGC GCTCTTC(1/4)
SfcI 1011 CTGTAG C^T[GA][CT]AG
SspI 1098 AATATT AAT^ATT
StyI 716 CCATGG C^C[AT][AT]GG
Tsp45I 549 GTCAC ^GT[GC]AC
XcmI 485 CCAGGCCATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 113 CTCGAG C^TCGAG
XhoII 1113 GGATCC [GA]^GATC[CT]
XmnI 343 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AvaI 113 CTCGAG C^[CT]CG[GA]G
586 CCCGAG
BbsI 133 GAAGAC GAAGAC(2/6)
928 GAAGAC
BpuAI 133 GAAGAC GAAGAC(2/6)
928 GAAGAC
BsaWI 602 TCCGGT [AT]^CCGG[AT]
1103 TCCGGA
BsoBI 113 CTCGAG C^[CT]CG[GA]G
586 CCCGAG
BsrFI 569 ACCGGC [GA]^CCGG[CT]
1055 GCCGGT
BstXI 172 CCAATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1118 CCAACGGACTGG
Cfr10I 569 ACCGGC [GA]^CCGG[CT]
1055 GCCGGT
DraII 478 AGGACCC [GA]G^G[ACGT]CC[CT]
657 GGGACCC
EcoO109I 478 AGGACCC [GA]G^G[ACGT]CC[CT]
657 GGGACCC
HgaI 90 GACGC GACGC(5/10)
739 GCGTC
MaeII 194 ACGT A^CGT
1031 ACGT
MaeIII 77 GTTAC ^GT[ACGT]AC
549 GTCAC
MseI 29 TTAA T^TAA
235 TTAA
MslI 322 CATTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
786 CACACGCGTG
MspA1I 592 CAGCGG C[AC]G^C[GT]G
799 CAGCTG
PflMI 948 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1317 CCAGGAGCTGG
PpuMI 478 AGGACCC [GA]G^G[AT]CC[CT]
657 GGGACCC
SfaNI 804 GATGC GCATC(5/9)
444 GCATC
SmlI 113 CTCGAG C^T[CT][GA]AG
208 CTCAAG
TfiI 381 GATTC G^A[AT]TC
1090 GAATC
Tru9I 29 TTAA T^TAA
235 TTAA
Van91I 948 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1317 CCAGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TaqI TseI Tsp509I
TspRI TthHB8I