Restriction map for
pF1KB4995
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EcoNI
EcoRI EcoRV EcoT22I FbaI FspI
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MscI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI Van91I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 49 GTCGAC GT^[AC][GT]AC
AccIII 716 TCCGGA T^CCGGA
AgeI 1352 ACCGGT A^CCGGT
Alw44I 148 GTGCAC G^TGCAC
Aor51HI 341 AGCGCT AGC^GCT
ApaI 1390 GGGCCC GGGCC^C
ApaLI 148 GTGCAC G^TGCAC
AspHI 148 GTGCAC G[AT]GC[AT]^C
AspI 700 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1460 GGCACC G^G[CT][GA]CC
BbsI 1834 GAAGAC GAAGAC(2/6)
BglI 1368 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1834 GAAGAC GAAGAC(2/6)
BsaI 438 GAGACC GGTCTC(1/5)
BseAI 716 TCCGGA T^CCGGA
BsgI 358 GTGCAG GTGCAG(16/14)
BsiHKAI 148 GTGCAC G[AT]GC[AT]^C
BspEI 716 TCCGGA T^CCGGA
BssSI 7 CACGAG CACGAG(-5/-1)
BstAPI 1021 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 905 GGTCACC G^GT[ACGT]ACC
BstPI 905 GGTCACC G^GT[ACGT]ACC
Eco47III 341 AGCGCT AGC^GCT
EcoO65I 905 GGTCACC G^GT[ACGT]ACC
FseI 1628 GGCCGGCC GGCCGG^CC
HincII 49 GTCGAC GT[CT]^[GA]AC
HindII 49 GTCGAC GT[CT]^[GA]AC
HindIII 31 AAGCTT A^AGCTT
MroI 716 TCCGGA T^CCGGA
MseI 191 TTAA T^TAA
NcoI 1850 CCATGG C^CATGG
NspI 750 GCATGC [GA]CATG^[CT]
PaeR7I 1158 CTCGAG C^TCGAG
PflFI 700 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1352 ACCGGT A^CCGGT
PvuII 1488 CAGCTG CAG^CTG
SalI 49 GTCGAC G^TCGAC
SapI 1970 GCTCTTC GCTCTTC(1/4)
SbfI 120 CCTGCAGG CCTGCA^GG
SphI 750 GCATGC GCATG^C
Sse8387I 120 CCTGCAGG CCTGCA^GG
Tru9I 191 TTAA T^TAA
Tsp509I 635 AATT ^AATT
TspRI 391 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 700 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1158 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 964 CGCG CG^CG
1635 CGCG
AocI 1455 CCTCAGG CC^T[ACGT]AGG
1720 CCTCAGG
BbvCI 1497 CCTCAGC CCTCAGC(-5/-2)
834 GCTGAGG
BglII 404 AGATCT A^GATCT
657 AGATCT
BsaWI 716 TCCGGA [AT]^CCGG[AT]
1352 ACCGGT
BsmBI 75 GAGACG CGTCTC(1/5)
1343 GAGACG
BstUI 964 CGCG CG^CG
1635 CGCG
Bsu36I 1455 CCTCAGG CC^T[ACGT]AGG
1720 CCTCAGG
Eco81I 1455 CCTCAGG CC^T[ACGT]AGG
1720 CCTCAGG
EcoT14I 755 CCAAGG C^C[AT][AT]GG
1850 CCATGG
MslI 609 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1324 CACTCAGGTG
MvnI 964 CGCG CG^CG
1635 CGCG
PstI 121 CTGCAG CTGCA^G
161 CTGCAG
SmaI 957 CCCGGG CCC^GGG
1098 CCCGGG
StuI 351 AGGCCT AGG^CCT
429 AGGCCT
StyI 755 CCAAGG C^C[AT][AT]GG
1850 CCATGG
TfiI 157 GATTC G^A[AT]TC
236 GATTC
XmaCI 957 CCCGGG C^CCGGG
1098 CCCGGG
XmaI 957 CCCGGG C^CCGGG
1098 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaI AvaII BanII BbvI
BcgI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TseI Tsp45I
TthHB8I XhoII