Restriction map for
pF1KB4982
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvrII
BamHI BbeI BcgI BciVI BclI
BglI BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
FbaI FseI HaeII Hin1I KasI
KpnI KspI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI PstI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 1144 CGCG CG^CG
AclI 586 AACGTT AA^CGTT
AflII 2020 CTTAAG C^TTAAG
ApaI 2260 GGGCCC GGGCC^C
Asp700I 1291 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1469 TGCGCA TGC^GCA
BanI 520 GGTGCC G^G[CT][GA]CC
BbrPI 2074 CACGTG CAC^GTG
BbvCI 963 CCTCAGC CCTCAGC(-5/-2)
BcnI 1485 CCGGG CC^[GC]GG
BfrI 2020 CTTAAG C^TTAAG
BglII 2319 AGATCT A^GATCT
BsaAI 2074 CACGTG [CT]AC^GT[GA]
BsmI 663 GCATTC GAATGC(1/-1)
Bsp1407I 2203 TGTACA T^GTACA
BsrGI 2203 TGTACA T^GTACA
BstUI 1144 CGCG CG^CG
BstXI 951 CCATGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 136 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1417 GAATTC G^AATTC
EcoRV 1549 GATATC GAT^ATC
FauI 2237 CCCGC CCCGC(4/6)
FspI 1469 TGCGCA TGC^GCA
HapII 1485 CCGG C^CGG
HgaI 739 GACGC GACGC(5/10)
HincII 1378 GTTAAC GT[CT]^[GA]AC
HindII 1378 GTTAAC GT[CT]^[GA]AC
HindIII 1790 AAGCTT A^AGCTT
HpaI 1378 GTTAAC GTT^AAC
HpaII 1485 CCGG C^CGG
MfeI 1256 CAATTG C^AATTG
MspI 1485 CCGG C^CGG
MunI 1256 CAATTG C^AATTG
MvnI 1144 CGCG CG^CG
NciI 1485 CCGGG CC^[GC]GG
NspI 836 GCATGT [GA]CATG^[CT]
PmaCI 2074 CACGTG CAC^GTG
PmlI 2074 CACGTG CAC^GTG
Psp1406I 586 AACGTT AA^CGTT
PvuII 49 CAGCTG CAG^CTG
SapI 1489 GAAGAGC GCTCTTC(1/4)
SspBI 2203 TGTACA T^GTACA
StuI 144 AGGCCT AGG^CCT
XmnI 1291 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 1417 GAATTC [GA]^AATT[CT]
1497 GAATTT
AlwNI 737 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
2327 CAGACTCTG
ApoI 1417 GAATTC [GA]^AATT[CT]
1497 GAATTT
BsaBI 442 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1627 GATGTGCATC
BsgI 1536 CTGCAC GTGCAG(16/14)
2393 CTGCAC
BspMI 1895 ACCTGC ACCTGC(4/8)
2251 ACCTGC
BsrDI 816 CATTGC GCAATG(2/0)
71 GCAATG
BstAPI 1649 GCAAGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2070 GCAGCACGTGC
BstEII 251 GGTTACC G^GT[ACGT]ACC
597 GGTTACC
BstPI 251 GGTTACC G^GT[ACGT]ACC
597 GGTTACC
BstYI 441 AGATCC [GA]^GATC[CT]
2319 AGATCT
CfoI 1145 GCGC GCG^C
1470 GCGC
DraIII 515 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
868 CACACTGTG
DsaI 1346 CCATGG C^C[GA][CT]GG
1737 CCATGG
EcoO65I 251 GGTTACC G^GT[ACGT]ACC
597 GGTTACC
EcoT22I 1 ATGCAT ATGCA^T
769 ATGCAT
HhaI 1145 GCGC GCG^C
1470 GCGC
HinP1I 1145 GCGC G^CGC
1470 GCGC
MaeII 587 ACGT A^CGT
2075 ACGT
MamI 442 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1627 GATGTGCATC
MflI 441 AGATCC [GA]^GATC[CT]
2319 AGATCT
MspA1I 49 CAGCTG C[AC]G^C[GT]G
2375 CAGCGG
NcoI 1346 CCATGG C^CATGG
1737 CCATGG
NsiI 1 ATGCAT ATGCA^T
769 ATGCAT
PflMI 549 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1821 CCACTTTATGG
PpuMI 171 GGGTCCC [GA]G^G[AT]CC[CT]
2060 AGGACCT
SacI 183 GAGCTC GAGCT^C
787 GAGCTC
TaqI 234 TCGA T^CGA
613 TCGA
TthHB8I 234 TCGA T^CGA
613 TCGA
Van91I 549 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1821 CCACTTTATGG
XcmI 669 CCAAATCCCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
863 CCATTCACACTGTGG
XhoII 441 AGATCC [GA]^GATC[CT]
2319 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI