Restriction map for
pF1KB4689
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsgI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraII DrdI
DsaI Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HpaI KasI KpnI KspI MamI
MluI MluNI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 667 CGCG CG^CG
AccIII 1027 TCCGGA T^CCGGA
Alw44I 590 GTGCAC G^TGCAC
ApaLI 590 GTGCAC G^TGCAC
AvaI 250 CCCGAG C^[CT]CG[GA]G
BamHI 1213 GGATCC G^GATCC
BbvI 1278 GCTGC GCAGC(8/12)
BglII 103 AGATCT A^GATCT
BpmI 914 CTGGAG CTGGAG(16/14)
BsaWI 1027 TCCGGA [AT]^CCGG[AT]
BseAI 1027 TCCGGA T^CCGGA
BseRI 803 CTCCTC GAGGAG(10/8)
BsiEI 819 CGGCCG CG[GA][CT]^CG
BsoBI 250 CCCGAG C^[CT]CG[GA]G
BspEI 1027 TCCGGA T^CCGGA
BspHI 1170 TCATGA T^CATGA
BstUI 667 CGCG CG^CG
CfoI 255 GCGC GCG^C
DdeI 569 CTGAG C^T[ACGT]AG
DraI 97 TTTAAA TTT^AAA
DraIII 1016 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 819 CGGCCG [CT]^GGCC[GA]
EagI 819 CGGCCG C^GGCCG
EclXI 819 CGGCCG C^GGCCG
Eco52I 819 CGGCCG C^GGCCG
EcoT14I 320 CCTTGG C^C[AT][AT]GG
FauI 693 GCGGG CCCGC(4/6)
HhaI 255 GCGC GCG^C
HinP1I 255 GCGC G^CGC
HindIII 463 AAGCTT A^AGCTT
MaeII 588 ACGT A^CGT
MfeI 287 CAATTG C^AATTG
MroI 1027 TCCGGA T^CCGGA
MslI 989 CATCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1150 CAGCGG C[AC]G^C[GT]G
MunI 287 CAATTG C^AATTG
MvnI 667 CGCG CG^CG
NdeI 1064 CATATG CA^TATG
PflMI 1015 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1170 TCATGA T^CATGA
SacI 836 GAGCTC GAGCT^C
SspI 190 AATATT AAT^ATT
StyI 320 CCTTGG C^C[AT][AT]GG
TseI 1278 GCTGC G^C[AT]GC
Tsp45I 350 GTGAC ^GT[GC]AC
Van91I 1015 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 52 GAATTT [GA]^AATT[CT]
296 AAATTC
AcyI 671 GACGCC G[GA]^CG[CT]C
737 GGCGTC
AfaI 206 GTAC GT^AC
330 GTAC
ApoI 52 GAATTT [GA]^AATT[CT]
296 AAATTC
AspHI 590 GTGCAC G[AT]GC[AT]^C
836 GAGCTC
BcnI 808 CCCGG CC^[GC]GG
822 CCGGG
BsaHI 671 GACGCC G[GA]^CG[CT]C
737 GGCGTC
BsaJI 320 CCTTGG C^C[ACGT][ACGT]GG
822 CCGGGG
BsiHKAI 590 GTGCAC G[AT]GC[AT]^C
836 GAGCTC
BsrI 221 CCAGT ACTGG(1/-1)
676 CCAGT
BstF5I 312 CATCC GGATG(2/0)
552 CATCC
BstXI 988 CCATCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1112 CCAAAGATTTGG
EcoNI 781 CCTCGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1204 CCTTTAGAAGG
Fnu4HI 818 GCGGC GC^[ACGT]GC
1278 GCTGC
FokI 312 CATCC GGATG(9/13)
552 CATCC
HgaI 671 GACGC GACGC(5/10)
738 GCGTC
Hin1I 671 GACGCC G[GA]^CG[CT]C
737 GGCGTC
HphI 49 GGTGA GGTGA(8/7)
1069 GGTGA
ItaI 818 GCGGC GC^[ACGT]GC
1278 GCTGC
MwoI 248 GCCCCGAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
738 GCGTCCCATGC
NciI 808 CCCGG CC^[GC]GG
822 CCGGG
PstI 68 CTGCAG CTGCA^G
1279 CTGCAG
RsaI 206 GTAC GT^AC
330 GTAC
SfcI 68 CTGCAG C^T[GA][CT]AG
1279 CTGCAG
XbaI 106 TCTAGA T^CTAGA
754 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BfaI BmyI BsaI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BssKI
BstNI BstYI Cfr13I DpnII EarI
EcoRII HaeIII HapII HinfI HpaII
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I XhoII