Restriction map for
pF1KB4636
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I AspEI
AvrII BbrPI BcgI BciVI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRV EcoT22I FseI
HincII HindII HpaI KspI MamI
MfeI MluI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI ScaI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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Acc65I 1576 GGTACC G^GTACC
AcyI 1564 GGCGCC G[GA]^CG[CT]C
Asp718I 1576 GGTACC G^GTACC
AspI 303 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2532 TGCGCA TGC^GCA
BalI 1434 TGGCCA TGG^CCA
BbeI 1564 GGCGCC GGCGC^C
BsaHI 1564 GGCGCC G[GA]^CG[CT]C
BsmBI 3129 GAGACG CGTCTC(1/5)
DraIII 247 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1421 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2675 GAATTC G^AATTC
FspI 2532 TGCGCA TGC^GCA
HgaI 3131 GACGC GACGC(5/10)
Hin1I 1564 GGCGCC G[GA]^CG[CT]C
HindIII 211 AAGCTT A^AGCTT
KasI 1564 GGCGCC G^GCGCC
KpnI 1576 GGTACC GGTAC^C
MluNI 1434 TGGCCA TGG^CCA
MscI 1434 TGGCCA TGG^CCA
NarI 1564 GGCGCC GG^CGCC
PflFI 303 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 1831 CCTGCAGG CCTGCA^GG
SexAI 248 ACCTGGT A^CC[AT]GGT
SfiI 1829 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1564 GGCGCC GGC^GCC
Sse8387I 1831 CCTGCAGG CCTGCA^GG
Tth111I 303 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 2500 GAATTT [GA]^AATT[CT]
2675 GAATTC
AocI 2833 CCTGAGG CC^T[ACGT]AGG
2956 CCTGAGG
Aor51HI 866 AGCGCT AGC^GCT
3338 AGCGCT
ApoI 2500 GAATTT [GA]^AATT[CT]
2675 GAATTC
BamHI 334 GGATCC G^GATCC
2185 GGATCC
BlpI 505 GCTCAGC GC^T[ACGT]AGC
2106 GCTCAGC
Bpu1102I 505 GCTCAGC GC^T[ACGT]AGC
2106 GCTCAGC
BspHI 372 TCATGA T^CATGA
1346 TCATGA
BsrBI 1739 CCGCTC CCGCTC(-3/-3)
1605 GAGCGG
BsrFI 600 ACCGGC [GA]^CCGG[CT]
1567 GCCGGT
BssSI 919 CACGAG CACGAG(-5/-1)
2690 CTCGTG
BstYI 334 GGATCC [GA]^GATC[CT]
2185 GGATCC
Bsu36I 2833 CCTGAGG CC^T[ACGT]AGG
2956 CCTGAGG
CelII 505 GCTCAGC GC^T[ACGT]AGC
2106 GCTCAGC
Cfr10I 600 ACCGGC [GA]^CCGG[CT]
1567 GCCGGT
EaeI 1434 TGGCCA [CT]^GGCC[GA]
2670 TGGCCG
Eco47III 866 AGCGCT AGC^GCT
3338 AGCGCT
Eco81I 2833 CCTGAGG CC^T[ACGT]AGG
2956 CCTGAGG
MaeII 2279 ACGT A^CGT
2798 ACGT
MflI 334 GGATCC [GA]^GATC[CT]
2185 GGATCC
MseI 125 TTAA T^TAA
2519 TTAA
RcaI 372 TCATGA T^CATGA
1346 TCATGA
SmlI 627 CTTGAG C^T[CT][GA]AG
2059 CTTGAG
Tru9I 125 TTAA T^TAA
2519 TTAA
Tsp509I 2501 AATT ^AATT
2676 AATT
XhoII 334 GGATCC [GA]^GATC[CT]
2185 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspEI BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MroI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI