Restriction map for
pF1KB4594
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII Aor51HI AscI AseI AsnI
Asp700I Asp718I AvaI AviII AvrII
BamHI BbrPI BbsI BcgI BcnI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsgI BsiWI BsoBI BspDI
BspHI BspLU11I BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI Eco47III EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HgaI
HindIII KpnI KspI MamI MfeI
MluI MunI NciI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 2074 CGCG CG^CG
AcsI 1781 GAATTC [GA]^AATT[CT]
AcyI 2044 GGCGCC G[GA]^CG[CT]C
AgeI 1705 ACCGGT A^CCGGT
AhdI 1270 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1422 GTGCAC G^TGCAC
AlwI 223 GATCC GGATC(4/5)
ApaI 1739 GGGCCC GGGCC^C
ApaLI 1422 GTGCAC G^TGCAC
ApoI 1781 GAATTC [GA]^AATT[CT]
AspEI 1270 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1509 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2044 GGCGCC GGCGC^C
BciVI 23 GTATCC GTATCC(6/5)
BclI 212 TGATCA T^GATCA
BlpI 1192 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1192 GCTGAGC GC^T[ACGT]AGC
BsaHI 2044 GGCGCC G[GA]^CG[CT]C
BsiEI 730 CGGCCG CG[GA][CT]^CG
BsmBI 70 CGTCTC CGTCTC(1/5)
BsrBI 679 GAGCGG CCGCTC(-3/-3)
BsrDI 1559 GCAATG GCAATG(2/0)
BstUI 2074 CGCG CG^CG
BstYI 222 AGATCC [GA]^GATC[CT]
CelII 1192 GCTGAGC GC^T[ACGT]AGC
DraIII 806 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 730 CGGCCG C^GGCCG
Eam1105I 1270 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 730 CGGCCG C^GGCCG
Eco52I 730 CGGCCG C^GGCCG
EcoRI 1781 GAATTC G^AATTC
FbaI 212 TGATCA T^GATCA
Hin1I 2044 GGCGCC G[GA]^CG[CT]C
HincII 1348 GTTAAC GT[CT]^[GA]AC
HindII 1348 GTTAAC GT[CT]^[GA]AC
HpaI 1348 GTTAAC GTT^AAC
KasI 2044 GGCGCC G^GCGCC
MflI 222 AGATCC [GA]^GATC[CT]
MvnI 2074 CGCG CG^CG
NarI 2044 GGCGCC GG^CGCC
NcoI 1250 CCATGG C^CATGG
PflFI 1509 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 709 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1705 ACCGGT A^CCGGT
SacI 2101 GAGCTC GAGCT^C
SapI 1935 GAAGAGC GCTCTTC(1/4)
ScaI 1520 AGTACT AGT^ACT
SfoI 2044 GGCGCC GGC^GCC
SmlI 1597 CTCAAG C^T[CT][GA]AG
SphI 1452 GCATGC GCATG^C
Tth111I 1509 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 709 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 219 TCTAGA T^CTAGA
XhoII 222 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 238 CCTGAGG CC^T[ACGT]AGG
1492 CCTGAGG
AvaII 144 GGTCC G^G[AT]CC
1355 GGTCC
BalI 317 TGGCCA TGG^CCA
659 TGGCCA
BbvCI 857 CCTCAGC CCTCAGC(-5/-2)
832 GCTGAGG
BfaI 220 CTAG C^TAG
1532 CTAG
BglI 4 GCCTCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1219 GCCTACACGGC
BsaI 912 GAGACC GGTCTC(1/5)
1507 GAGACC
BsmI 1053 GAATGC GAATGC(1/-1)
1164 GAATGC
BssSI 1214 CACGAG CACGAG(-5/-1)
416 CTCGTG
BstAPI 1424 GCACGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1605 GCACACGCTGC
Bsu36I 238 CCTGAGG CC^T[ACGT]AGG
1492 CCTGAGG
DsaI 320 CCACGG C^C[GA][CT]GG
1250 CCATGG
Eco81I 238 CCTGAGG CC^T[ACGT]AGG
1492 CCTGAGG
HaeII 511 AGCGCC [GA]GCGC^[CT]
2044 GGCGCC
MaeI 220 CTAG C^TAG
1532 CTAG
MaeII 1426 ACGT A^CGT
1486 ACGT
MluNI 317 TGGCCA TGG^CCA
659 TGGCCA
MscI 317 TGGCCA TGG^CCA
659 TGGCCA
PvuII 18 CAGCTG CAG^CTG
2014 CAGCTG
StuI 1589 AGGCCT AGG^CCT
1655 AGGCCT
Tsp509I 286 AATT ^AATT
1782 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwNI
AspHI BanI BanII BbvI BmyI
BpmI BsaJI BsaWI BseAI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspEI BspMI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MroI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NdeII NgoMIV NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I