Restriction map for
pF1KB4563
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI AspI AvrII BalI
BamHI BbeI BbrPI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BstEII BstPI Bsu36I
ClaI CpoI DraI DrdI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FseI
HincII HindII HpaI KasI Ksp632I
MamI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NdeI
NotI NsiI PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 531 GTATAC GT^[AC][GT]AC
AhdI 38 GACCCGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 522 GGATC GGATC(4/5)
AspEI 38 GACCCGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI 1936 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 392 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 392 GCTTAGC GC^T[ACGT]AGC
BsaAI 2089 TACGTG [CT]AC^GT[GA]
BsaI 2104 GAGACC GGTCTC(1/5)
BsmBI 1615 CGTCTC CGTCTC(1/5)
BssSI 116 CTCGTG CACGAG(-5/-1)
Bst1107I 531 GTATAC GTA^TAC
BstYI 522 GGATCT [GA]^GATC[CT]
BstZ17I 531 GTATAC GTA^TAC
CelII 392 GCTTAGC GC^T[ACGT]AGC
Eam1105I 38 GACCCGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 861 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2458 GATATC GAT^ATC
HindIII 1225 AAGCTT A^AGCTT
KspI 41 CCGCGG CCGC^GG
MflI 522 GGATCT [GA]^GATC[CT]
NheI 2038 GCTAGC G^CTAGC
NruI 760 TCGCGA TCG^CGA
PaeR7I 245 CTCGAG C^TCGAG
SacI 856 GAGCTC GAGCT^C
SacII 41 CCGCGG CCGC^GG
SexAI 650 ACCTGGT A^CC[AT]GGT
XhoI 245 CTCGAG C^TCGAG
XhoII 522 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 3 GGCGTC G[GA]^CG[CT]C
2434 GACGCC
AflIII 1193 ACATGT A^C[GA][CT]GT
2277 ACATGT
ApaI 626 GGGCCC GGGCC^C
1973 GGGCCC
Asp700I 1210 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2604 GAAACAGTTC
AspHI 856 GAGCTC G[AT]GC[AT]^C
1870 GAGCAC
AviII 2141 TGCGCA TGC^GCA
2594 TGCGCA
BbsI 1600 GTCTTC GAAGAC(2/6)
2281 GTCTTC
BcgI 2259 CGAGTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1814 GCAACTTTATCG
BciVI 213 GGATAC GTATCC(6/5)
1527 GGATAC
BclI 1385 TGATCA T^GATCA
2621 TGATCA
BfaI 1375 CTAG C^TAG
2039 CTAG
BpuAI 1600 GTCTTC GAAGAC(2/6)
2281 GTCTTC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
2434 GACGCC
BsiHKAI 856 GAGCTC G[AT]GC[AT]^C
1870 GAGCAC
BspLU11I 1193 ACATGT A^CATGT
2277 ACATGT
BsrBI 18 GAGCGG CCGCTC(-3/-3)
136 GAGCGG
BstBI 257 TTCGAA TT^CGAA
1217 TTCGAA
BstXI 1754 CCAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2321 CCACCCGCCTGG
FbaI 1385 TGATCA T^GATCA
2621 TGATCA
FspI 2141 TGCGCA TGC^GCA
2594 TGCGCA
HaeII 2659 AGCGCC [GA]GCGC^[CT]
2678 GGCGCT
Hin1I 3 GGCGTC G[GA]^CG[CT]C
2434 GACGCC
MaeI 1375 CTAG C^TAG
2039 CTAG
MseI 584 TTAA T^TAA
1460 TTAA
NaeI 1810 GCCGGC GCC^GGC
2503 GCCGGC
NgoMIV 1810 GCCGGC G^CCGGC
2503 GCCGGC
NspV 257 TTCGAA TT^CGAA
1217 TTCGAA
PpuMI 36 GGGACCC [GA]G^G[AT]CC[CT]
2309 AGGTCCT
SfuI 257 TTCGAA TT^CGAA
1217 TTCGAA
SmaI 914 CCCGGG CCC^GGG
1205 CCCGGG
Tru9I 584 TTAA T^TAA
1460 TTAA
XcmI 995 CCACAGCTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2540 CCAAGGGCGATGTGG
XmaCI 914 CCCGGG C^CCGGG
1205 CCCGGG
XmaI 914 CCCGGG C^CCGGG
1205 CCCGGG
XmnI 1210 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2604 GAAACAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwNI ApoI Asp718I AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I