Restriction map for
pF1KB4445
##################################
# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmAI BsmBI BsmI BsoBI Bsp1286I
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cac8I CelII
Cfr10I CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AciI 308 CCGC CCGC(-3/-1)
AfaI 427 GTAC GT^AC
AvaII 185 GGACC G^G[AT]CC
BcnI 198 CCCGG CC^[GC]GG
BsaAI 322 CACGTA [CT]AC^GT[GA]
Bsp1407I 426 TGTACA T^GTACA
BspDI 391 ATCGAT AT^CGAT
BsrGI 426 TGTACA T^GTACA
BstXI 89 CCAAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 305 GCGC GCG^C
Cfr13I 185 GGACC G^G[ACGT]CC
ClaI 391 ATCGAT AT^CGAT
DdeI 17 CTGAG C^T[ACGT]AG
EarI 418 GAAGAG CTCTTC(1/4)
EcoT14I 89 CCAAGG C^C[AT][AT]GG
HaeII 304 AGCGCC [GA]GCGC^[CT]
HapII 199 CCGG C^CGG
HgaI 318 GCGTC GACGC(5/10)
HhaI 305 GCGC GCG^C
HinP1I 305 GCGC G^CGC
HpaII 199 CCGG C^CGG
HphI 83 TCACC GGTGA(8/7)
Ksp632I 418 GAAGAG CTCTTC(1/4)
MaeII 323 ACGT A^CGT
MaeIII 320 GTCAC ^GT[ACGT]AC
MboII 418 GAAGA GAAGA(8/7)
MspA1I 10 CAGCTG C[AC]G^C[GT]G
MspI 199 CCGG C^CGG
MwoI 296 GCTACATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 198 CCCGG CC^[GC]GG
NlaIII 213 CATG CATG^
NlaIV 184 GGGACC GG[ACGT]^[ACGT]CC
PstI 145 CTGCAG CTGCA^G
PvuII 10 CAGCTG CAG^CTG
RsaI 427 GTAC GT^AC
Sau96I 185 GGACC G^G[ACGT]CC
SfaNI 169 GATGC GCATC(5/9)
SfcI 145 CTGCAG C^T[GA][CT]AG
SspBI 426 TGTACA T^GTACA
StuI 44 AGGCCT AGG^CCT
StyI 89 CCAAGG C^C[AT][AT]GG
TaqI 392 TCGA T^CGA
Tsp45I 320 GTCAC ^GT[GC]AC
Tsp509I 412 AATT ^AATT
TthHB8I 392 TCGA T^CGA
XcmI 86 CCACCAAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 113 GATCC GGATC(4/5)
255 GATCC
BalI 218 TGGCCA TGG^CCA
402 TGGCCA
BbvI 144 GCTGC GCAGC(8/12)
315 GCTGC
BclI 155 TGATCA T^GATCA
374 TGATCA
BstYI 112 AGATCC [GA]^GATC[CT]
254 AGATCC
EaeI 218 TGGCCA [CT]^GGCC[GA]
402 TGGCCA
FbaI 155 TGATCA T^GATCA
374 TGATCA
MflI 112 AGATCC [GA]^GATC[CT]
254 AGATCC
MluNI 218 TGGCCA TGG^CCA
402 TGGCCA
MscI 218 TGGCCA TGG^CCA
402 TGGCCA
TseI 144 GCTGC G^C[AT]GC
315 GCTGC
XhoII 112 AGATCC [GA]^GATC[CT]
254 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BsaJI BseRI BsmFI BssKI
BstF5I BstNI DpnII EcoRII Fnu4HI
FokI HaeIII ItaI MboI MnlI
MvaI NdeII Sau3AI ScrFI TspRI